2001 Fiscal Year Final Research Report Summary
Molecular mecbanism for determination ofdynamics and positioning in nucleus of vertebrates
Project/Area Number |
12440212
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
遺伝
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Research Institution | Natiomal Institute of Genetics |
Principal Investigator |
IKEMURA Toshimichi Natiomal Inst.of Genetics, Dept.of Popul.Genet, Professor, 集団遺伝研究系, 教授 (50025475)
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Co-Investigator(Kenkyū-buntansha) |
FUKAGAWA Tatsuo Natiomal Inst.of Genetics,Dept.of Popul.Genet,Assis.Prof, 集団遺伝研究系, 助手 (60321600)
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Project Period (FY) |
2000 – 2001
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Keywords | Triplex / Polypurine / CENP / FISH / Nuclear organization / chromosome band boundary / Replication timing / DNA複製タイミング |
Research Abstract |
The single-strand formation and transmolecular triplex formation are thought to enable sequences spaced distantly along the genome to associate with each other and organize nuclear DNA into ordered configurations. Triplex-forming DNAs in the human interphase nucleus were analyzed by combining immunodetection by antitriplex antibodies and fluorescence in situ "nondenaturing " hybridization employing polypurine/polypyrimidine (PuPy) -tract probes. The "nondenaturing" hybridization technique, which has been used to detect RNA, could detect single-stranded DNAs in nondenatured nuclei. Triplexes visualized differentially with distinct PuPy-tract probes were associated spatially with centromere sequences in the interphase nucleus in a sequence-specific manner. Therefore, we were interested in centromore formation and function. To determine the precise functions of centromere proteins, we examined their roles by generating a conditional loss-of-function mutant in the chicken DT40 cell line. We concluded that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere. We also performed living cell analysis of cells expressing GFP-or YFP- tagged centromere proteins to observe the dynamics of chromosomes.
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Research Products
(24 results)
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[Publications] Watanabe, Y, Fujiyama, A, Ichiba, Y7 Hattori, M., Yada, T., Sakaki, Y and Ikemura, T.: "Chromosome-wide assessment of replication timing for human chromosomes llq and 21q : disease-related genes in timing-switch regions."Human Molecular Genetics. 11. 13-21 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sonoda, E., Matsusaka, X, Morrison, C, Vagnarelli, P., Hoshi, O., Ushiki, T., Nojima, K., Fukagawa, T., Waizenegger, I.C Peters, J-M., Earnshaw, W.C. and Shunichi Tafceda, S: "Socl/Rad21/MCpl is required for sister chrotnatid cohesion and kinetoehore function in vertebrate cells."Dev. Cell. 1. 759-770 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kanaya, S., Kinouchi M., Abe, T., Kudo, Y, Yamada, Y, Nislii, T., Mori, Mori, H. and Ikemura, T: "Analysis of codon usage diversity of bacterial gertes with a self-organizing map (SOM) : characterjzation of horizpiitally transferred genes with emphasis on the E. call O157 genome"Gene. 276. 89-99 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fukagawa, T., Mikami, Y, Nishihashi, A, Regnier, V., Haraguehi, T, Hiraoica, Y, Sugata, N., Todokoro, K., Brown, W., and Ikemura, T: "CENP-H, a constitutive centromere component, is required for centromere targeting of GENP-C in vertebrate cells."The BMBO Journal. 20. 4603-4617 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shina, T., Ando, A, Suto, Y, Kasai, F., Shigenari, A, Takishima, N., Kikkawa, E., Iwata, K., Kuwano, Y, Kitamura, Y, Watsuzawa, Y, Sano, K., Nogami, M., Kawata, H., Li, S., Eukuzumi, Y, Yamazaki, M., Tashiro, H., tamiya, G, Kohda, A, Okumura, K., Ikemura, T., Soeda, E., Mizuki, N., Kimura, M., Bahram, S., and Inoko, H.: "Genoinic anatomy of apremier major histocompatibihty complex paralogous region on chromosome Iq21-q22"Genome Research. 11. 7879-802 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nogami, M., Nogami, O., Kagotani, K., Taguchi, H., Ikemura, T. and Okumura, K: "Intranuclear arrangement of human chromosome 12 correlates to large-scale replication domains."Chrotnosoma. 108. 514-522 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nogami, M., Kohda, A, Taguchi, H., Nakao, M., Ikemuraj T., Okumura, K: "Relative locations of the centromere and imprinted SNKPN gene'within chromosome 15 territories during the cell cycle in HL60 cells."Journal of Cell Scienc. 113. 2157-2165 (2000)
Description
「研究成果報告書概要(欧文)」より