2002 Fiscal Year Final Research Report Summary
MOLECULAR BIOLOGY ON CYANOBACTERIAL CO_2 PUMPS
| Project/Area Number |
12440228
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
OGAWA Teruo NAGOYA UNIVERSITY, BIOSCIENCE CENTER, PROFESSOR, 生物分子応答研究センター, 教授 (80087593)
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| Co-Investigator(Kenkyū-buntansha) |
OMATA Tatsuo NAGOYA UNIVERSITY GRADUATE COURSE OF BIOAGRICULTURAL SCIENCES, PROFESSOR, 生命農学研究科, 教授 (50175270)
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| Project Period (FY) |
2000 – 2002
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| Keywords | CO_2-concentrating mechanism / CO_2 pump / NADPH dehydrogenase / Bicarbonate transporter / SbtA-dependent type / Cmp-dependent type / NdhD3-dependent type / NdhD4-dependent type |
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| Research Abstract |
We have identified two CO_2-uptake systems, one constitutive dependent on NdhD3/NdhF3/CupA(Sll 1734) and one low-CO_2-inducible dependent on NdhD4/NdhF4/CupB(Slr 1302), and two types of HCO_3^- transporters, an ABC-type transporter encoded by cmp operon and a novel sodium-dependent transporter encoded by slr 1512 (sbtA) that plays a central role in HCO_3^- uptake in the cyanobacterium Synechocystis sp, strain PCC 6803. Mutants impaired for one of these 4 inorganic carbon-acquisition systems did not show mutant phenotype. Mutants inactivated for both CO_2-uptake systems were unable to grow at pH 7.0 in air although they grew normally at pH 9.0 in air. Additional inactivation of the SbtA-type HCO_3^- transporter abolished the growth at pH 9.0 in air. Using the antibody raised against SbtA, we have shown that SbtA is localized in the cytoplasmic membrane. Immunoprecipitation using this antibody revealed that a protein of about 10 kDa was coprecipitated with SbtA. Amino-acid sequencing revealed that this protein the product of slr 1513 (named as sbtB) cotranscribed with sbtA.
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[Publications] Tchernov, D., Helman, Y, Keren, N., Luz, B., Ohad, V, Reinhold, L., Ogmwa, T., Kaplan, A.: "Passive entry of CO_2 and its intracellular conversion to HCO_3^-in cyanobacteria are driven by a photosystem I-generated ΔμH^+"J. Biol Chem.. 276. 23450-23455 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Shibata, M., Ohkawa, H., Kaneko, T., Fukuzawa, H., Tabata, S., Kaplan, A., Ogawa, T.: "Distinct constitutive and low CO_2-induced CO_2 uptake systems in cyanobacteria : Genes involved and their phylogenetic relationship with homologous genes in other organisms"Proc. Natl. Acad. Sci. USA. 98. 11789-11794 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Shibata, M., Katoh, H., Sonoda, M., Ohkawa, H, Shimoyama.M., Fukuzawa, H., Kaplan, A., Ogawa, T.: "Genes essential to sodium-dependent bicarbonate transport in cyanobacteria : Function and phylogenetic analysis"J. Biol. Chem.. 277. 18658-18664 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tchernov, D., Helman, Y., Keren, N., Luz, B., Ohad, I., Reinhold, L., Ogawa, T.and Kaplan, A.: "Passive entry of CO_2 and its intracellular conversion to HCO_3^- in cyanobacteria are driven by a photosystem I-generated ΔμH^+"J.Biol.Chem.. 276. 23450-23455 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shibata, M., Ohkawa, H., Kaneko, T., Fukuzawa, H., Tabata, S., Kaplan, A.and Ogawa, T.: "Distinct constitutive and low CO_2-induced CO_2 uptake systems in cyanobacteria : Genes involved and their phylogenetic relationship with homologous genes in other organisms"Proc.Natl.Acad.Sci.USA. 98. 11789-11794 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ohkawa, H., Sonoda, M., Hagino, N., Shibata,M., Pakrasi, H.B.and Ogawa, T.: "Functionaly distinct NAD(P)H dehydrogenases and their membrane localization in Synechocystis sp.PCC 6803"Funct.Plant Biol.. 29. 195-200 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shibata, M., Katoh, H., Sonoda, M., Ohkawa, H., Shimoyama, M., Fukuzawa, H., Kaplan, A.and Ogawa, T.: "Genes essential to sodium-dependent bicarbonate transport in cyanobacteria : Function and phylogenetic analysis"J.Biol.Chem.. 277. 18658-18664 (2002)
Description
「研究成果報告書概要(欧文)」より
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