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2002 Fiscal Year Final Research Report Summary

The possibility of nitrogen fixation by genetic improving the rice chioroplast

Research Project

Project/Area Number 12460001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

TSUTSUMI Nobuhiro  THE UNIVERSITY OF TOKYO, The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor (00202185)

Co-Investigator(Kenkyū-buntansha) YOSHIBA Yoshu  株式会社日立製作所, Hitachi Ltd., Life Sci.Res.Center., Project Leader (researcher)
Project Period (FY) 2000 – 2002
Keywordsstromule / ribosomal protein / GFP / Kaede / protein transport / mitochondria
Research Abstract

In order to visualize the shapes of plastids in a living cell, a fusion gene of GFP with the transit peptide of rice chloroplast ribosomal protein L12-1 (cpTP-GFP) was introduced into rice epidermal cells by the particle bombardment method. Confocal laser scanning microscopy showed that the GFP fluorescence was localized in the plastids.
The cpTP-GFP signals were matched specially with the signals of chlorophyll autofluorescence. In addition, there were some cells that had plastids with various lengths of protuberances without chlorophyll, i.e., "stromules". In a few cells, stromules connected almost all the plastids in a cell, appearing as a pan-cytoplasmic network. One plausible function of stromule connections is to exchange plastid components such as proteins. This would unify or coordinate all of the plastids in a cell and, by lessening the demands on the nucleus, contribute to the productivity of the cell.
To determine whether fusion of plastids mediated by stromules occurs, we labeled plastids in onion epidermal cells with a plastid-targeted fluorescent protein (Kaede) and then altered the fluorescence of some of the plastids within a cell from green to red. No transition to a uniform color (yellow) indicating the fusion of plastids was observed, even two days after the photo-conversion.

  • Research Products

    (13 results)

All 2003 2002 2001 2000 Other

All Journal Article (13 results)

  • [Journal Article] A unified nomenclature for Arabidopsis dynamin-related large GTPases based on homology and possible functions.2003

    • Author(s)
      Hong Z.
    • Journal Title

      Plant Mol. Biol. 53

      Pages: 261-265

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A unified nomenclature for Arabidopsis dynamin-related large GTPases based on homology and possible functions.2003

    • Author(s)
      Hong, Z., Bednarek, S.Y., Blumwald, E., Hwang, I., Jurgens, G., Menzel, D., Osteryoung, K.W., Raikhel, N.Y., Shinozaki, K., Tsutsumi, N., Verma, D.P.S.
    • Journal Title

      Plant Mol.Biol. 53

      Pages: 261-265

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A dynamin-like protein (ADL2b), rather than FtsZ, is involved in Arabidopsis mitochondrial division.2002

    • Author(s)
      Arimura S.
    • Journal Title

      Proc. Nat1. Acad. Sci. USA 99

      Pages: 5727-5731

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A dynamin-like protein (ADL2b), rather than FtsZ, is involved in Arabidopsis mitochondrial division.2002

    • Author(s)
      Arimura, S., Tsutsumi, N.
    • Journal Title

      Proc.Natl.Acad.Aci.USA 99

      Pages: 5727-5731

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Numerous and highly developed tubular projections from plastids observed in Tobacco epidermal cells.2001

    • Author(s)
      Arimura S.
    • Journal Title

      Plant Sci. 160

      Pages: 449-454

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of four extension genes in Arabidopsis thaliana by differential gene expression under stress and non-stress conditions.2001

    • Author(s)
      Yosbiba Y
    • Journal Title

      DNA Res. 8

      Pages: 115-122

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Numerous and highly developed tubular projections from plastids observed in Tobacco epidermal cells.2001

    • Author(s)
      Arimura, S., Hirai, A., Tsutsumi, N.
    • Journal Title

      Plant Sci. 160

      Pages: 449-454

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of four extension genes in Arabidopsis thaliana by differential gene expression under stress and non-stress conditions.2001

    • Author(s)
      Yoshiba, Y., Aoki, C., Iuchi, S., Nanjo, T., Seki, M., Sekiguchi, F., Yamaguchi-Shinozaki, K., Shinozaki, K.
    • Journal Title

      DNA Res. 8

      Pages: 115-122

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the alternative oxidase 1a gene.2000

    • Author(s)
      Tsuji H.
    • Journal Title

      FEBS Lett. 471

      Pages: 201-204

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions.2000

    • Author(s)
      Nakazono M.
    • Journal Title

      Plant Physiol. 124

      Pages: 587-598

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: evidence for involvement of calcium in expression of the alternative oxidase la gene.2000

    • Author(s)
      Tsuji, H., Nakazono, M., Saisho, D., Tsutsumi N., Hirai, A.
    • Journal Title

      FEBS Lett 471

      Pages: 201-204

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions.2000

    • Author(s)
      Nakazono, M., Tsuji, H., Li, Y., Saisho, D., Arimura, S., Tsutsumi, N., Hirai, A.
    • Journal Title

      Plant Physiol 124

      Pages: 587-598

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arabidopsis dynamin-like protein 2a (ADL2a), like ADL2b, is involved in plant mitochondrial division.

    • Author(s)
      Arimura, S., Aida, G.P., Fujimoto, M., Nakazono, M., Tsutsumi, N.
    • Journal Title

      Plant and Cell Physiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2011-06-18  

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