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2001 Fiscal Year Final Research Report Summary

Expression mechanism of insecticide-insensitive acetylcholinesterase which has no amino asid replacement

Research Project

Project/Area Number 12460020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionUniversity of Tsukuba

Principal Investigator

KONO Yoshiaki  Univ. of Tsukuba, Inst. Of Agriculture and Forestry, Professor, 農林学系, 教授 (10225386)

Co-Investigator(Kenkyū-buntansha) TOMITA Takashi  Nad. Inst. Of Infectious Diseases, Dep. Of MedEntomol, Head, 昆虫医科学部, 室長 (20180169)
Project Period (FY) 2000 – 2001
Keywordsorganophosphorus insecticide / insecticide resistance / acetylchilinesterase / Insensitiviry / molecular mechanism / amino asid replacement
Research Abstract

The AChE of the toyama strain of Culex tritaenlorhynchus showed extremely low sensitivity to various kinds of organophosphates (OP) and carbamates compared to a susceptible strain, Taiwan. An open reading frame encoding 633 residues of AChE precursor was determined from cDNA sequence. The deduced protein sequences of AChE were identical among Toyama and Taiwan. A cloned AChE cDNA was introduced to BmNPV-BmN4 expression system and AChE sensitive to OP was produced regardless of cDNA sources; susceptible or resistant strains.
To the contrary, AChE of OP resistant strains of the house fly has 2 amino acids replacement in the structure. Expression experiments of AChE gene of resistant strains confirmed mat the insensitivity to the insecticides was caused by the replacement of the amino acids.
Therefore, there are two different molecular mechanism to produce insensitive AChE. The one is merely expression of the structural gene which maps insensitive AChE in it. This type of insensitive AChE was observed in D. melanogaster and M. domestica. The other is a production of two different structured AChEs from the same DNA map. The post translational modification of the protein is inevitable by other factors. According to our results, this type of insensitive AChE seems to distribute widely in insect species, C. tritaeniorhynchua, Nephotettix cincticepus, Qulema oryzae, Myzus persicae, Aphis gossipii, Tetranychtis ulticae, Plutella xyllostella. Linkage analysis of AChE atructure gene, the gene relating insensitivity of AChE and other molecular marker genes was conducted in C. tritaeniorhynchus, and indicated that the gene concerning the insensitivity located on chromosome 2 which was not the same chromosome loaded with AChE structure, gene. This result suports the existance of the second mechanism.
Purification of sensitive and insensitive AChE were tried to provide the materials for furthere studies on the mechanism of insensitive AChE production in insects.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Tomita, T., O.Hidoh, Y.Kono: "Absence of protein polymorphism attributable to insectiside-insensitibity of acetylcholinesterase in the green rice leafhopper, Nephotettix cincticeps"InsectBiochom. Mol. Biol.. 30. 325-333 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kozaki, T., T.Tomita, T.Shono, Y.Kono: "Fenitrothion insensitive acetylchol-inesterases of the housefly, Musca domestica associated with point mutation"Insect Biochem. Mol. Biol.. 31. 991-997 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kozaki, T., T.Tomita, T.Shono, Y.Kono: "Polymorphism in the acetylcholinesterase gene of the housefly, Musca domestica L."Appl. Entomol. Zool.. 36. 377-380 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori.A., T.Tomita., O.Hidoh., Y.Kono, D.W.Severson: "Comparative linkage map development and identification of an autosomal locus for insensitive acetylcholinesterase-mediated insecticide resistance in ('ulex tritaenyorhynchus"Insect Mol. Biol.. 20. 197-203 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kozaki, T., T.Hono, T.Tomita, D.Taylor, Y.Kono: "Linkage analysis of an acetylcholinesterase gene in the housefly, Musca domestica L."J. Econ. Entomol.. 95(印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野義明: "アセチルコリンエステラーゼの感受性低下による殺虫剤抵抗性"植物防疫. 55. 316-320 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomita, T., O. Hidoh, Y. Kono: "Absence of protein polymorphism attributable to insectiside-insensitibity of acelylcholinesterase in the green rice leafliopper, Nephoteltix cincticeps"Insect Biochem.Mol. Biol. 30. 325-333 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kozaki, T., T. Tomita, T.Shono, Y.Kono: "Fenitrothion insensitive acetylchol-inesterases of the housefly, Musca domestics associated with point mutation"Insect Biochem.Mol. Biol. 31. 991-997 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kozaki, T., T. Tomita, T. Shono, Y. Kono: "Polymorphism in the acetylcholinesterase gene of the housefly, Musca domexlica L"Appl.Entomol.Zool. 36. 377-380 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, A., T. Tomita, O. Hidoh, Y. Kono, D. W. Severson: "Comparative linkage map development and identification of an autosomal locus for insensitive acelylcholinesterase-mediated insecticide resistance in Culex trilaenyarhynchus"Insect Mol. Biol. 20. 197-203 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kozaki, T., T. Shono, T. Tomita, D. Taylor, Y. Kono: "Linkage analysis of an acetylcholinesterase gene in the housefly, Musca domestica L"J.Econ.Entomol. 95 (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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