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2002 Fiscal Year Final Research Report Summary

Studies on morphogenesis of infection structure and signal network in plant pathogenic fungi - regulatory mechanisms of environmental response and gene expression

Research Project

Project/Area Number 12460024
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKYOTO PREFECTURAL UNIVERSITY

Principal Investigator

KUDO Yasuyuki  Laboratory of Plant Pathology, Faculty of Agriculture, Professor, 農学研究科, 教授 (80183797)

Project Period (FY) 2000 – 2002
KeywordsColletotrichum / Infection structure / Appressoria / Morphogenesis / Signal transduction / Gene expression / Response to environment / Plant pathogenic fungus
Research Abstract

1) Isolation and characterization of genes involved in morphogenesis of infection structure and signal network
The C. lagenarium GMK1 gene, a homologue of the Saccharomyces cerevisiae FUS3/KSS1 MAP kinase genes, was shown to regulate conidial germination, appressorium formation, and invasive growth. In S. cerevisiae, Ste12p is known to be a transcriptional factor downstream of Fus3p/Kss1p MAP kinases. In this research, to evaluate the CMK1 MAP kinase pathway, the Ste12 homologue CST1 gene from C. lagenarium was isolated and characterized. cst1Δ strains were nonpathogenic on intact host leaves, but could form lesions when inoculated on wounded leaves. Conidia of the cst1Δ strains could germinate and form melanized appressoria on both host leaf surface and artificial cellulose membrane, but could not produce infectious hyphae from appressoria, suggesting that CST1 is essential for appressorium penetration in C. lagenarium. In addition, matured appressoria of the cst1Δ strains contained ex … More tremely low level of lipid droplets, compared with that of the wild type strain. Lipid droplets were abundant in conidia of the cst1Δ strains, but rapidly disappeared during appressorium formation. This misscheduled lipid degradation might be related to the failure of appressorium penetration in the cst1Δ strain.
2) Establishment of Agrobacterium tumefaciens-mediated transformation as an experimental tool for random insertional mutagenesis in Colletotrichum lagenarium
In this research, Agrobacterium tumefociens-mediated transformation (AtMT) was applied to Colletotrichum lagenarium for random insertional mutagenesis. Optimal co-cultivation of C. lagenarium wild-type 104-T with pBIG2RHPH2, a binary vector carrying hygromycin-resistant gene cassette, introduced A. tumefaciens C58C1 led to good production of hygromycin-resistant transformants. The fungal genomic DNA segments flanking T-DNA could be identified from several pathogenicity or morphogenesis deficient mutants by thermal asymmetric interlaced-polymerase chain reaction (TAIL-PCR). Sequence from one of the mutants was identical with melanin biosynthesis gene PKS1 of C. lagenarium that we have previously characterized. These results indicated that AtMT could be an effective tool for tagging genes relevant to pathogenicity and morphogenesis in the plant pathogenic fungus C. lagenarium. Less

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Tsuji G.: "Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and pig1p of Magnaporthe grisea, contain Cys2His2 zinc finger and Zn (II)2Cys6 binuclear cluster"Molecular Microbiology. 38. 940-955 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujii I.: "Enzymatic synthesis of 1,3,6,8-tetrahydroxynaphthalene solely from malonyl coenzyme A by a fungal interactive typeI polyketide synthase PKS1"Biochemistry. 39. 8853-8858 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto S.: "Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum lagenarium"Microbiology. 147. 2623-2628 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji G.: "Expression of melanin biosynthesis enzymes during infectious morphogenesis of Colletotrichum lagenarium"Journal of General Plant Pathology. 69(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji G.: "The Colletotrichum lagenarium Ste12-like gene CST1 is essential for appressorium penetration"Molecular Plant-Microbe Interaction. 16(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji G.: "Agrobacterium tumefaciens-mediated transformation for random insertional mutagenesis in Colletotrichum lagenarium"Journal of General Plant Pathology. 69(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubo Y.: "Host Specificity and Pathogenicity and Host Pathogen Interaction of Colletotrichum"APS Press, St.Pual Minnesota. 14 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gento Tsuji: "Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and Pig1p of Magnaporthe grisea, contain Cys2His2 zinc finger and Zn(II)2Cys6 binuclear cluster DMA binding motifs, and regulate transcription of development specific manner"melanin biosynthesis genes in a Molecular Microbiology. 38. 340-955 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isao Fujii: "Enzymatic synthesis of 1,3,6,8-tetrahydroxynaphthalene solely from malonyl coenzyme A by a fungal iterative type I polyketide synthase PKS1"Biochemistry. 39. 8853-8858 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gento Tsuji: "Laccase gene LAC1 of Colletotrichum lagenorium is not essential for melanin biosynthesis and pathogenicity"Journal of General Plant Pathology. 67. 182-190 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Susumu Okamoto: "Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum lagenarium"Microbiology. 147. 2623-2628 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gento Tsuji: "Expression of melanin biosynthesis enzymes during infectious morphogenesis of Colletotrichum lagenarium"Journal of General Plant Pathology. 69:in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gento Tsuji: "Agrobacterium tumefaciens-mediated transformation for randam insertional mutagenesis in Colletotrichum lagenarium"Journal of General Plant Pathology. 69:in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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