2002 Fiscal Year Final Research Report Summary
Study on host-recognition mechanism of root parasitic weeds
| Project/Area Number |
12460049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | UTSUNOMIYA UNIVERSITY |
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Principal Investigator |
YONEYAMA Koichi Utsunomiya University, Center for Research on Wild Plants, Professor, 野生植物科学研究センター, 教授 (00114174)
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| Co-Investigator(Kenkyū-buntansha) |
NATSUAKI Tomohide Utsunomiya University, Faculty of Agriculture, Professor, 農学部, 教授 (10134264)
OGASAWARA Masaru Utsunomiya University, Center for Research on Wild Plants, Associate Professor, 野生植物科学研究センター, 助教授 (40194419)
TAKEUCHI Yasutomo Utsunomiya University, Center for Research on Wild Plants, Professor, 野生植物科学研究センター, 教授 (90008003)
SASAKI Izumi Oyama National College of Technology, Materials Chemistry and Bioengineering, 物質工学科, 助手 (70270220)
YOKOTA Takao Teikyo University, Faculty of Science and Technolgy, Professor, 理工学部, 教授 (40011986)
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| Project Period (FY) |
2000 – 2002
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| Keywords | root parasitic weeds / germination stimulants / haustorium inducing compounds / host recognition mechanism / cell wall degrading enzymes / ethylene biosynthesis / genetic analysis / LC / MS |
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| Research Abstract |
Germination stimulants produced by various hosts of root parasitic weeds have been examined. For example, soybean and Arabidopsis were found to produce orobanchol. In addition, production of orobanchol by red clover roots has been quantified by a newly developed LC/MS/MS method. Some hosts, including tomato, pea, and sorghum were found to produce novel strigolactones. ACC synthase and ACC oxidase genes were cloned from Orobanche minor, Although Orobanche ACC oxidase gene had a high homology with genes from other plants, Orobanche ACC synthase gene contained several stop codons in its N-terminal region and thus may not be functional. Among cell wall degrading enzymes of O. Minor, beta-glucosidase was most abundant. This enzyme may be involved in penetration into host roots by degrading cell walls and also by inhibiting host defense system.
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