2002 Fiscal Year Final Research Report Summary
Studies on signal transduction pathways of jasmonic acid in suspension-cultured rice cells
| Project/Area Number |
12460051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
YAMANE Hisakazu THE UNIVERSITY OF TOKYO, The University of Tokyo, Biotechnology Research Center, Professor (80090520)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Makoto The University of Tokyo, Biotechnology Research Center, Associate Professor (00208240)
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| Project Period (FY) |
2000 – 2002
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| Keywords | suspension-cultured rice cell / defense response gene / signal transduction / jasmonic acid-responsive gene / 12-oxophytodienoic acid reductase / transcription factor / phytoalexin / diterpene cyclase |
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| Research Abstract |
In this project, we have tried to isolate and characterize jasmonic acid (JA)-responsive genes to elucidate the signal transduction pathways of JA in suspension-cultured rice cells. In addition, molecular probes for JA receptors were also synthesized.
The JA-responsive genes RRJ1 and RRJ2 were found to encode a putative rice cystathionine γ-lyase and a pyruvate decarboxylase, respectively. RRJ1 is possibly involved in biosynthesis and/or metabolism of sulfur-containing amino acids and related biochemical processes, and RRJ2, in energy metabolism for elicitor-induced defense reactions in suspension-cultured rice cells.
We succeeded in cloning of the JA-responsive gene OsOPR1 encoding 12-oxophytodienoic acid (OPDA) reductase (OPR) that is one of key biosynthetic enzymes of JA. This is the first cloning of an OPR gene from a monocot plant. Since OsOPR1 catalyze reduction of (-)-cis-OPDA preferentially over (+)-cis-OPDA, a natural precursor of JA, OsOPR1 might contribute to other physiologic
… More
al phenomena than JA biosynthesis.
RERJ1 was isolated as an early JA-responsive gene which encodes a transcriptional regulator possessing a basic region/helix-loop-helix motif. Based on analysis of phenotypes of sense- or antisense-RERJ1 mRNA overexpressing transgenic rice plants, RERJ1 is suggested to be involved in JA-induced inhibition of shoot growth in rice. Involvement of RERJ1 in other physiological phenomena including defense response is also quite possible. Futher analysis of biological functions of RERJ1 is in progress.
JA functions as a key signal transducer in a chitin elicitor-induced diterpenoid phytoalexin production in suspension-cultured rice cells. We isolated a cDNA encoding a diterpene cyclase from the chitin-elicited suspension-cultured rice cells, and demonstrated that the gene product functioned as ent-cassa-12, 15-diene synthase. This is the first report of isolation of a cDNA encoding a diterpene cyclase involved in biosynthesis of a diterpene hydrocarbon which is a putative precursor of diterpenoid phytoalexins in rice. ent-Cassa-12, 15-diene synthase cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of biosynthesis of the rice diterpenoid phytoalexins (-)-phytocassanes during plant development and by change of environmental conditions.
We synthesized several types of JA derivatives including JA-amino acid conjugates, JA-biotin conjugate, JA-dexamethasone heterodimer, and JA-fluoresceine conjugate as candidates for molecular probes for JA-binding proteins. Since these synthesized compounds except JA-fluoresceine conjugate exhibited significant JA-like activities in rice seedling assay, soybean PAL-inducing assay, and/or rice momilactone A-inducing assay, they might be useful probes to isolate and characterize JA-binding proteins. Less
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[Journal Article] Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor.2004
Author(s)
E.-M.Cho, A.Okada, H.Kenmoku, T.Toyomasu, T.Sassa, A.Yajimal, G.Yabutai, K.Mori, H.Oikawa, H.Toshima, N.Shibuya, T.Omori, M.Nishiyama, H.Yamane
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Journal Title
The Plant Journal 37
Pages: 1-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells.2003
Author(s)
H.Sobajima, M.Takeda, M.Sugimori, N.Kobashi, K.Kiribuchi, E.-U.Cho, C.Akimoto, T.Yamaguchi, E.Minami, N.Shibuya, F.Schaller, E.W.Weiler, T.Yoshihara, H.Nishida, H.Nojiri, T, Omori, M.Nishiyama, H.Yamane
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Journal Title
Planta 216
Pages: 692-698
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cloning and characterization of cDNAs for the jasmonic acid-responsive genes RRJ1 and RRJ2 in suspension-cultured rice cells.2002
Author(s)
M.Sugimori, K.Kiribuchi, C.Akimoto, T.Yamaguchi, E.Minami, N.Shibuya, E.-U.Cho, N.Kobashi, H.Nojiri, T, Omori, M.Nishiyama, H.Yamane
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Journal Title
Bioscience, Biotechnolnology, and Biochemistry 66
Pages: 1140-1142
Description
「研究成果報告書概要(欧文)」より
