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2002 Fiscal Year Final Research Report Summary

Molecular and Pathological Factors involved in Sustainable Wood Quality

Research Project

Project/Area Number 12460078
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KURODA Hiroyuki  KYOTO UNIVERSITY WOOD RESEARCH INSTITUTE INSTRUCTOR, 木質科学研究所, 助手 (00115841)

Co-Investigator(Kenkyū-buntansha) KURODA Keiko  FORESTRY AND FORESTPRODUCT INSTITUTE, KANSAI CENTER GROUP LEADER, 関西支所, グループ長 (20353675)
HARA Masakazu  SHIZUOKA UNIVERSITY, ASSISTANT FACARITY OF AGRICULTURE ASSISTANT PROFESSOR, 農学部, 助手 (10293614)
KUBOI Toru  SHIZUOKA UNIVERSITY, FACULTY OF AGRICULTURE PROFESSOR, 農学部, 教授 (20132847)
Project Period (FY) 2000 – 2002
KeywordsSECONDARY METABOLITES / STILBENOID BIOSYNTHESIS / COLD TOLERANCE / DEHYDRIN / CHATETIN / Mn STRESS / XYLEM DYSFUNCTION / MASS MORTALITY
Research Abstract

Generally speaking, a tree produces sustainable wood under physiologically normal condition. When a tree is physiologically apart far from the normal condition, it may produce disordered wood. In other words, biotic and abiotic stresses disturb "sustainable" wood quality. Various genetic factors are involved in the process, or normal "sustainable" wood formation. Among the stress factors, we focused on anti-fungal and cold tolerant genes. Against the biotic stress, stilbene synthases have characterized by using heterologous expression after the cDNA cloning. Against the abiotic stress, dehydrin has cloned, being characterized by physiological, transgenic, and molecular studies. We have presented a reliable molecular basis on the "sustainable" wood quality or wood formation, in relation to anti-fungal and cold torelant factors.
In vitro culture system makes complex abiotic analyses rather simple. Here, the effect of abiotic stress, especially temperature and a heavy metal, had examined. The culture system responded to produce secondary metabolites, especially catechins, in order to keep physiologically normal condition as far as possible. Furthermore, the secondary metabolites played an important role for scavenging active oxygen and detoxification of Mn ions.
The field surveys on the health of conifer plantations were made covering wide area from the subarctic to temperate zones. In addition to microscopic analysis of wood specimens, physiological aspects were checked in diseased trees, for instance with acoustic emission technique (AE). The results indicated that the water conductivity of sapwood is most important to keep trees healthy.
Altogether, the studies have revealed molecular and pathological bases in forest health and healthiness in the wood quality, or sustainable wood quality.

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Hara, M., Terashima, S., Fukaya, T., Kuboi, T.: "Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco"Planta (Published online : 15 February 2003). (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kodan, A., Kuroda, H., Sakai F.: "A stilbene synthase from Japanese red pine (Pinus densiflora) : Implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis"Proc.Natl.Acad.Sci.USA.. 99(5). 3335-3339 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiko Kuroda, Y.Kanbara, T.Inoue, A.Ogawa: "Analysis of NMR-CT images to detect the xylem dysfunction and lesions in tree trunks"IAWA Journal. 23. 469-470 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroda, K.: "The mechanism of tracheid cavitation in trees infected with wilt diseases"Proceedings of the IUFRO Working party 7.02.02 Shoot and foliage diseases, Meeting at Hyytiala, Finland. 43. 17-23 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kodan, A., Kuroda, H., Sakai, F.: "Simultaneous expression of stilbene synthase genens in Japanese red pine (Pinus densiflora) seedlings"J.Wood Science. 47(1). 58-62 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara, M., Terashima, S., Kuboi, T.: "Characterization and cryoprotective activity of cold-responsive dehydrin from Citrus unshiu"J.Plant Physiol.. 158. 1333-1339 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroda, K.: "Responses of Quercus sapwood to infection with the pathogenic fungus of a new wilt disease vectored by the barkbeetle Platypus quercivorus"J.Wood Science. 47. 425-429 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kodan, A., Kuroda, H., Sakai, F: "Stilbene synthase from Japanese red pine, its role in stilbenoid biosynthesis"Proceedings of the 3^<rd> international wood Science Symposium. 258-263 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 黒田宏之(分担執筆): "木の誕生;夢の培養木材を目指して"ブルーバックス 木材なんでも小事典(木質科学研究所木悠会編)(講談社). 16-23-309-312 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 黒田慶子(分担執筆): "スネにきず....過去は隠せない?"森に学ぶ101のヒント(日本林業技術協会編)(東京書籍). 106-107 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimomdra. F., Sakai. F. and Kuroda, H.: "O-methyltransferase (OMT) cDNA clones from Pinus densiflora seedlings"Wood Research. 89. 11-12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kodan, A., Kuroda, H. and Sakai, F.: "A stilbene synthase from Japanese red pine (Pinusdensflora): Implications for phytoalexin acctunulation and down-regulation of flavonoid biosynthesis"Proc Natl Acad Sci U S A. 99(5). 3335-3339 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kodan, A., Kuroda, H., Sakai, F.: "Simultaneous expression of stilbene synthase genens in Japanese red pine (Pinus densflora) seedlings"J. Wood Sci.. 47(1). 58-62 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Etoh, K., A. Kodan, F. Sakai and H. Kuroda: "An O-methyltransferase (GMT) cDNA clone in Japanese red pine (Pinus densflora) seedlings"Wood Research. 87. 8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kodan, A., Kuroda, H. and Sakai, F: "Stilbene synthase from Japanese red pine, its role in stilbenoid biosynthesis"Proceedings of the 3^<rd> international wood Science Symposium. 258-263 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara, M. Terashima, S. Ftaya. T., and Kuboi, T.: "Enhancement of cold tolerance and inhibibon of lipid peroxidation by cihus dehydrin in transgenic tobacco"Planta. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara, M., Terashima, S., and Kuboi, T.: "Characterization and cryoprotective activity of cold-responsive dehydrin from Citrus unshiu"J. Plant Physiol.. 158. 1333-1339 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuroda, K. Y. Kanbara, T. Inoue. and A. Ogawa: "Analysis of NMR-CT images to detect the xylem dysfunction and lesions in tree bunks"IAWA Journal. 23. 469-470 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuroda, K.: "Responses of Quercus sapwood to infection with the pathogenic fungus of a new wilt disease vectored by the barkbeetle Platypus quercivorus"J. Wood Science. 47. 425-429 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuroda, K.: "The mechanism of tracheid cavitation in trees infected with wilt diseases"Proceedings of te IUFRO Working party 7. 02. 02 Shoot and foliage diseases, Meeting at Hyytialaj Finland. 17-23 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuroda, K.: "Anatomical assessment of age of infection with resinous stem canker in hinoki cypress (Chamaecyparis obtusa) and the factors promoting resinosis"Mokuzai Gakkaishi. 46. 503-509 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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