• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

Development of an efficient system for cryopreservation of oocytes and embryos by use of ultrarapid vitrification

Research Project

Project/Area Number 12460126
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKochi University

Principal Investigator

KASAI Magosaburo  Kochi University, Faculty of Agriculture, Professor, 農学部, 教授 (60152617)

Co-Investigator(Kenkyū-buntansha) SUGIMOTO Miki  Kyoto University, Faculty of Agriculture, Assistant, 大学院・農学研究科, 助手 (20243074)
EDASHIGE Keisuke  Kochi University, Faculty of Agriculture, Associate professor, 農学部, 助教授 (30175228)
Project Period (FY) 2000 – 2002
Keywordsembryo / oocyte / cryopreservation / vitrification / ovary / channel / cryoprotectant / 耐凍剤
Research Abstract

The present study aimed to examine the efficacy of ultrarapid vitrification and to develop new strategies for the cryopreservation of mammalian oocytes and embryos, based on the understanding of fundamental cryobiology.
Ultrarapid vitrification using the cryoloop was effective for human blastocysts, in which conventional vitrification does not yield consistent results. For mouse oocytes, the post-warming survival after vitrification using cryoloops was not different from that after vitrification using conventional straws. For rat embryos, in which the efficacy of vitrification has not reached a level for practical use, conventional straw vitrification using EFS solution was quite effective, on the condition that embryos at suitable stages of development are used. As a tool to identify the mechanism of injury, we specified morphological characteristics of embryos that is typical to specific types of injuries, such as intracellular ice formation and cryoprotectant toxicity. As a potential … More strategy for cryopreservation, we tried to increase the permeability of the cell membrane artificially. We injected cRNA of a certain type of aquaporins, a water channel that can transport not only water but also cryoprotectants, into mouse oocytes. In oocytes injected with cRNA of aquaporin 3, both water permeability and glycerol permeability increased significantly. These oocytes survived after vitrification in a solution based on glycerol, which scarcely permeates into intact oocytes, whereas none of oocytes injected with water survived after vitrification in the solution. The oocytes in which the plasma membrane was temporarily 'altered' retained the ability to be fertilized and to develop to term.
The results obtained in this study would be valuable for the cryopreservation of embryos and oocytes in domestic animals, laboratory animals and humans. In addition, a new strategy found in this study would have potential advantage for the cryopreservation of various genetic resources that need to be preserved. Less

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Shaw, Jillian M.: "Embryo cryopreservation for transgenic mouse lines"Methods in Molecular Biology. 158. 397-419 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mukaida, Tetsunori: "Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique"Fertility and Sterility. 76・3. 618-620 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kasai, Magosaburo: "Advances in the cryopreservation of mammalian oocytes and embryos : Development of ultrarapid vitrification"Reproductive Medicine and Biology. 1・1. 18-26 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kasai, Magosaburo: "Morphological appearance of the cryopreserved mouse blastocyst as a tool to identify the type of cryoinjury"Human Reproduction. 17・7. 1863-1874 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Edashige, Keisuke: "Artificial expression of aquaporin-3 improves the survival of mouse oocytes after cryopreservation"Biology of Reproduction. 68・1. 87-94 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mukaida, Tetsunori: "Vitrification of human blastocysts using cryoloops : clinical outcome of 223 cycles"Human Reproduction. 18・2. 384-391 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Han, Myung-Sook: "Vitrification of rat embryos at various developmental stages"Theriogenology. 59・8. 1851-1863 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木秋悦, 佐藤英明編: "「卵子研究法」卵子の凍結保存法,受精卵・胚,マウス"養賢堂. 327-336 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jill Shaw, and Magosaburo Kasai.: "Embryo cryopreservation for transgenic mouse lines"Methods in Molecular Biology. 158. 397-419 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsunori Mukaida, Sanae Nakamura, Tatsuhiro Tomiyama, Shigeko Wada, Magosaburo Kasai, and Katsuhiko Takahashi: "Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique"Fertility and Sterility. 76. 618-620 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Magosaburo Kasai.: "Advances in the cryopreservation of mammalian oocytes and embryos : development of ultrarapid vitrification"Reproductive Medicine and Biology. 1. 1-9 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Magosaburo Kasai, Kaori Ito, and Keisuke Edashige.: "Morphological appearance of the cryopreserved mouse blastocyst as a tool to identify the type of cryoinjury"Human Reproduction. 17. 1863-1874 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keisuke Edashige, Yohei Yamaji, F. W. Kleinhans, and Magosaburo Kasai.: "Artificial expression of aquaporin-3 improves the survival of mouse oocytes after cryopreservation"Biology of Reproduction. 68. 87-94 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsunori Mukaida, Sanae Nakamura, Tatsuhiro Tomiyama, Shigeko Wada, Chikahiro Oka, Magosaburo Kasai, and Katsuhiko Takahashi.: "Vitrification of human blastocysts using cryoloops : clinical outcome of 223 cycles"Human Reproduction. 18. 384-391 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Myung-Sook Han, Koji Niwa, and Magosaburo Kasai.: "Vitrification of rat embryos at various developmental stages"Theriogenology. 59. 1851-1863 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2004-04-14  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi