2002 Fiscal Year Final Research Report Summary
Induction mechanisms of mucosal immune response in intramucosal parasitic protozoan infection
| Project/Area Number |
12460136
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE |
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Principal Investigator |
OMATA Yoshitaka Obihiro University of Agriculture and Veterinary Medicine, Department of Veterinary Physiology, Associated Professor, 畜産学部, 助教授 (10132987)
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| Co-Investigator(Kenkyū-buntansha) |
UDUKA Yuji Obihiro University of Agriculture and Veterinary Medicine, Department of Veterinary Physiology, Associated Professor, 畜産学部, 助教授 (30151913)
MAKINO Souichi Obihiro University of Agriculture and Veterinary Medicine, Department of Veterinary Physiology, Associated Professor, 畜産学部, 助教授 (30181621)
SAITO Atsushi Obihiro University of Agriculture and Veterinary Medicine, Department of Veterinary Physiology, Professor, 畜産学部, 教授 (10002263)
FURUOKA Hidefumi Obihiro University of Agriculture and Veterinary Medicine, Department of Veterinary Physiology, Associated Professor, 畜産学部, 助教授 (60238665)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Infection / Mucosal immunity / Defense mechanism / Immune response / Cryptosporidium parvum / Eimeria stiedai / Toxoplasma gondii / Neospora caninum |
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| Research Abstract |
This study was aimed to clarify induction mechanism of mucosal immune responses in tissues invaded by parasitic protozoa and relationship between systemic immune responses and cell mediated immune responses. In addition, specific antigen molecules derived from the parasites were also attempted to analyze the immunologic and biological characteristics.
Analysis of characteristics of Cryptosporidium parvum soluble antigen
Soluble antigen was detected in the supernatant of feces of calves infected with C. parvum. The infected calves produced IgA antibody against the copro-antigen during convalescent phase of the infection.
Analysis of feline enteroepithelial stages antigens in Toxoplasma gondii
From enteroepithelia stage parasites of T. gondii, cDNA library was constructed and 14 clones were isolated by immunoscreening. Sequence analysis and function assay showed similarity to 14-3-3 protein.
Characteristics of Eimeria stiedai soluble antigen and immune responses in rabbits either infected or immunized with the antigen
Soluble antigen was detected in the bile of infected rabbits. Rabbits infected with E. stiedai produced antibody to the antigen. Rabbits immunized with the soluble antigen showed resistant to the infection.
Pathogenicity of Neospora caninum isolated from sheep and immune responses to the infection in mice
Neospora caninum was isolated from sheep and the pathogenicity was examined by experimental infection in mice. Immunodeficient mice showed clinical signs to die. Immunocompetent mice showed without any clinical signs and produce antibody against the parasites.
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