2001 Fiscal Year Final Research Report Summary
Evaluation methods for hygienic safety of activated sludge from sewage plants
| Project/Area Number |
12460146
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源科学
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| Research Institution | Nihon University |
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Principal Investigator |
NAKAJIMA Matsuyasu Nihon University, College of Bioresauce Sciences, professor, 生物資源科学部, 教授 (10059660)
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| Co-Investigator(Kenkyū-buntansha) |
SUNAIRI Michio Nihon University, College of Bioresauce Sciences, associate professor, 生物資源科学部, 助教授 (80196906)
UWATOKO Kazuhiro Nihon University, College of Bioresauce Sciences, lecturer, 生物資源科学部, 講師 (70223625)
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| Project Period (FY) |
2000 – 2001
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| Keywords | activated sludge / poliovirus / non-specific adhesion / extracellular polysaccharides / membrane protein |
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| Research Abstract |
To recycle activated sludge generated at sewer plants it is important to protect workers who handle the activated sludge from potential risk of pathogenic microorganisms in the sludge as well as to provide recycled products safe to human health and environments. Although the understanding how these pathogenic microorganisms adhere to activated sludge is essential for that purpose the knowledge is still limited.
In this study, we conducted experiments as described below for that purpose
We characterized physicochemical natures of cell surface of the gastric-ulcer related pathogen, Helicobacter pylori, and revealed that coccoid-form cells possessed higher hydrophilic cell surface than helical-shaped cells of mid-exponential phase.
We enumerated enteric viruses in activated sludge samples taken from sewer plants located at Tokyo and its suburbs. The majorities of virus adhered to activated sludge, however, small numbers of infectious virus particles were also detected from discharge water. The viral numbers seemed to change seasonally.
Hydrophobic interaction is one of the important factors of microbial adhesion. We had already reported that the extracellular polysaccharides (EPS) inhibit the adhesion of Rhodococcus rhodochrous to activated sludge. We determined the chemical composition of the rhodococcal EPS. A simple method to analize outer membrane proteins of Rhodococci was also established.
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