2001 Fiscal Year Final Research Report Summary
Neurogenesis in the ischemic adult rat brain
| Project/Area Number |
12470140
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUMOTO Masayasu Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助教授 (20192346)
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUKI Toshiho Osaka University Hospital, Medical Staff, 医学部・附属病院, 医員
MATSUSHITA Kohji Osaka University Hospital, Medical Staff, 医学部・附属病院, 医員
KITAGAWA Kazuo Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (70301257)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Neurogenesis / Cerebral Ischemia / Neural Stem Cells / Hippocampus / Musashi 1 / rat / Aging / Bromodeoxyuridine |
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| Research Abstract |
Recently, there has been great interest in adult neurogenesis. We investigated whether transient forebrain ischemia could influence the proliferation of neuronal progenitor cells in the subgranular zone (SGZ) of the rat hippocampus after transient forebrain ischemia and permanent focal ischemia. Male Wistar rat were subjected to 4-vessel occlusion or middle cerebral artery occlusion. We used a bromodeoxyuridine (BrdU) labeling method to identify the postproliferation cells and double-immunostaining with confocal microscopy to determine the cell phenotype. The number of BrdU-positive cells in the SGZ incased several fold 8 days after both forebrain and focal ischemia. BrdU-positive cells formed clusters and expressed Musashi1 (Msi1), a marker of neural stem/progenitor cells, but only 10-20 % of BrdU-positive cells expressed glial fibrillary acidic protein (GFAP), a marker of astrocytes. In young animals, transient forebrain ischemia resulted in increased number of BrdU-positive cells 4 weeks after BrdU injection. About 70-80 % of all BrdU-positive cells in the dentate gyrus pressed NeuN, a marker of matured neuron. Although proliferation of progenitor cells was stimulated in older animals, and in both ischemic and nonischemic hippocampus, survival of newborn cells after ischemic stress markedly reduced in older animals and in nonischemic hippocampus. These results showed that ischemia stimulated the proliferation of neuronal progenitor cells, but did not support survival of newborn cells in older animals and in the contralateral hippocampus.
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[Publications] Takasawa K, Kitagawa K, Yagita Y, Sasaki T, Tanaka S, Matsushita K, Ohtsuki T, Miyata T, Okano H, Hori M, Matsumoto M: Journal of Cerebral Blood Flow and Metabolism. (in press). (2002)
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