2002 Fiscal Year Final Research Report Summary
Enhancement of radiotherapy and hyperthermia by transfection with cancer suppressor gene
| Project/Area Number |
12470189
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Nara Medical University |
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Principal Investigator |
OHNISHI Takeo Nara Medical University, Department of Biology, Professor, 医学部, 教授 (60094554)
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| Project Period (FY) |
2000 – 2002
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| Keywords | gene therapy / p53 / radiation / gyperthermia / anti-cancer agent / apoptosis / gene transfection / DNA ladder |
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| Research Abstract |
We examined p53 gene therapy combined with radiation or hyperthermia using several human cancer cell lines transfected with wild-type p53 (wtp53) or mutated p53 gene (mp53). Transfection with wtp53 to p53-null lung cancer cells (H1299) increased the radiosensitivity and enhanced radiation-induced apoptosis. Human anaplastic thyroid carcinoma cells (8305c bearing mp53) transfected with temperature sensitive mp53 showed an enhanced radiation or heat sensitivity and apoptosis under 32℃ culture condition of expression of wtp53 phenotype. Human squamous cell carcinoma (SAS bearing wtp53 phenotype) cells transfected with rsp53 were resistant to X-ray compared with the control cells having normal function of p53 (SAS/neo). SAS/neo tumor-transplanted nude mice showed a delayed rumor growth compared with SAS/mp53 tumor-transplanted nude mice after treatment with X-ray or hyperthermia. From these results, p53 gene therapy combined with radiation and hyperthermia therapies might improve the outcome of cancer therapies.
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[Publications] Takahashi, A, K. Ohnishi, X. Wang, M. Kobayashi, H, Matsumoto, T. Tamamoto, H. Aoki, Y. Furusawa, O. Yukawa & T. Ohnishi: "The dependence of p53 on the radiation-enhancement of thermosensitivity at different LET"Int. J. Radiat. Oncol. Biol. Phys.. 47. 489-494 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takahashi, A.., K. Ohnishi, K. Matsumoto, H. Aoki, X. Wang, T. Tamamoto, O. Yukawa, Y. Furusawa, Y. Ejima, A. Tachibana & T. Ohnishi: "WAF1 accumulation by carbon-ion beam and a-particle irradiation in human glioblastoma cultured cells"Int J. Radiat Biol.. 76. 335-341 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takahashi, A.., I. Ota, T. Tamamoto, I. Asakawa, Y. Nagata, H. Nakagawa, N. Kondo, K. Ohnishi, Y. Furusawa, H. Matsumoto & T. Ohnishi: "p53-Dependent hyperthermic ; enhancement of tumor growth inhibition by X-ray or Carbon-ion beam irradiation,"Int J. Hyperthermia. 19. 145-153 (2003)
Description
「研究成果報告書概要(欧文)」より
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