2002 Fiscal Year Final Research Report Summary
HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE
| Project/Area Number |
12470217
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Meiji University |
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Principal Investigator |
KATO Yukio MEIJI UNIVERSITY, SCHOOL OF AGLICULTURE, PROFESSOR, 農学部, 教授 (30114177)
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| Project Period (FY) |
2000 – 2002
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| Keywords | PITUITARY / RECOMBINANT DNA / GONADOTROPIN / CLONING / TRANSCRIPTION FACTOR / GENE / HOMEOBOX / YEST ONE HYBRID |
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| Research Abstract |
(1) FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.
(2) THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.
(3) PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSH β GENE
(4) EXPRESSION VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHβ GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.
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[Publications] Chen L, Maniyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y and Inoue K: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neural nitric oxide synthetase expression and nitric oxide secretrion."Endocrinology. 141. 3603-3310 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tomizawa K, Ogawa S, Kato T, Watanabe M, Sato I, Sakai T, Hattori M-A, Inoue K, Ueda S, Kikuchi M, Mori J and Kato Y.: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev. 48. 131-136 (2002)
Description
「研究成果報告書概要(欧文)」より
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