G protein diseases : analysis and application

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12470218
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: The University of Tokyo
• Principal Investigator: IIRI Taroh Tokyo Univ., Dept. of Medicine Assist. Prof., 医学部・附属病院, 助手 (90313022)
• Co-Investigator(Kenkyū-buntansha): OHNISHI Hirohide Tokyo Univ., Dept. of Medicine Prof., 医学部・附属病院, 助手 (00313023) ; MOTOKURA Tohru Tokyo Univ., Dept. of Medicine Prof., 医学部・附属病院, 講師 (00192823) ; FUJITA Toshiro Tokyo Univ., Dept. of Medicine Prof., 医学部・附属病院, 教授 (10114125)
• Project Period (FY): 2000 – 2001
• Keywords: G protein / Receptor / Molecular Medianism / G protein disease / Gene therapy

Research Abstract

G protein diseases have taught us a great deal about how G proteins work. By taking advantage of the notion and model we proposed (Nature, 394 : 35-39, 1998), we demonstrated the followings.
(1) Clarification of the mechanism of a new PHP-Ia mutant, αs-AVDT,
(2) We extended the model of receptor-dependent motivation of G proteins.
(3) Design of dominant positive/negative G protein α submits.
(4) We made simple method of tranfection using DEAE adenovirus.

Research Products

• [Publications] Vitalis EA et al.: "Role of the cAMP signaling pathway in the regulation of gonadotropin-releasing hormone secretion in GT1 cells"Proc. Natl. Acad. Sci. USA. 97. 1861-1860 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shimosawa T et al.: "Adrenomedullin Amidation Enzyme Activities in Hypertensive Patients"Hypertens Res.. 23. 167-173 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hansen JL et al.: "Functional reconstitution of the angiotensin II type 2 receptor and G(i) activation"Circ. Res.. 87. 753-759 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Suzuki J. et al.: "Dynamin is involved in human epithelial cell vacuolation caused by the Helicobactre pylori-produced cytotosin VacA"J. Clin. Invest. 107. 363-370 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Rondard, P et al.: "Mutant G protein a subunit activated by Gb g : a model for receptor activation?"Proc. Natl. Acad. Sci. USA. 98. 6150-6155 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] E A Vitalis, J L Costantin, P-S Tsai, H Sakakibara, S Paruthiyil, T liri, J-F Martini, M Taga, A L H Choi, A C Charles and R I Weiner: "Role of the cAMP signaling pathway in the regulation of gonadotropin-releasing hormone secretion in GT1 cells"Proc. Natl. Acad. Sci. U.S.A.. 97. 1861-1866 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T Shimosawa, K Kanosawa, R Nagasawa, T Mitarai, K Isoda, K Takahashi, K Ando, Y Tozawa, M Nagase, N Sasaki, M Fujita, K Takano, T liri and T Fujita: "Adrenomedullin amidation enzyme activities in hypertensive patients"Hypertens. Res.. 23. 167-173 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] J L Hansen, G Servant, T J Baranski, T Fujita, T Irii, S Haunso and S P Sheikh: "Functional reconstitution of the Angiotensin II type 2 receptor and Gi activation"Circ. Res.. 87. 753-759 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] J Suzuki, H Ohnishi, H Shibata, A Wada, T Hirayama, T liri, N Ueda, C Kanamaru, T Tsuchida, H Mashima, H Yasuda and T Fujita: "Dynamin is involved in the vacuolation induced by Helicobacter Pylori vacuolating cytotoxin (VacA) in human epithelial cells"J. Clin. Invest.. 107. 363-370 (2001)
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• [Publications] P Rondard, T liri, S Srinivasan, E Meng, T Fujita and H R Bourne: "A G_<sα> mutant activated by Gβγ suggests a molecular mechanism for activation of trimeric G-proteins"Proc. Natl. Acad. Sci. U.S.A.. 98. 6150-6155 (2001)
  • Description: 「研究成果報告書概要(欧文)」より