2001 Fiscal Year Final Research Report Summary
Prognostic Score of Gastric Cancer Determined by cDNA Microarray
| Project/Area Number |
12470241
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
INOUE Hiroshi Medical Institute of Bioregulation, Kyushu Univ., Associate Professor, 生体防御医学研究所, 助教授 (90203249)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Masaki Medical Institute of Bioregulation, Kyushu Univ., Professor, 生体防御医学研究所, 教授 (70190999)
SADANAGA Noriaki Medical Institute of Bioregulation, Kyushu Univ., Research Associate, 生体防御医学研究所, 助手 (20304826)
SHIBUTA Kenji Medical Institute of Bioregulation, Kyushu Univ., Research Associate, 生体防御医学研究所, 助手 (70253531)
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| Project Period (FY) |
2000 – 2001
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| Keywords | DNA microarray / gene expression / Gastric cancer / prognostic score / tumor behavior |
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| Research Abstract |
To estimate gastric cancer patients' prognosis more comprehensively, we tried to develop a prognostic scoring system using cDNA microarray. RNA was extracted from tumor/normal paired samples of 43 patients with gastric cancer, and cDNA microarray hybridization was performed. Consequently, we selected 78 genes that were differentially expressed between aggressive and non-aggressive groups with respect to five conventional pathologic factors. Next, we determined a coefficient for each gene. Thereafter a prognostic score was calculated by summing-up the value for each gene. It ranged from -47 to 201 with a median of 114. There were two peaks in its distribution. Ten of 11 patients who were alive with no evidence of recurrence more than 5 years after the operation showed a score of less than 100 point, while all 19 patients who died of disease showed more than 100 point. In 13 patients who were alive but the follow-up time was less than 5 years, two of the 3 patients with more than 100 point revealed recurrent disease during the follow up. These findings demonstrate that such a system with cDNA microarray can contribute to the comprehensive analysis of malignant behavior of the tumor and may provide accurate information on prognosis.
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Research Products
(14 results)
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[Publications] Etoh, T., Inoue, H., Tanaka, S., Barnard, G. F., Kitano, S., and Mori, M.: "Angiopoietin-2 is related to tumor angiogenesis in gastric carcinoma : possible in vivo regulation via induction of proteases"Cancer Res.. 61. 2145-53 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuyama, A., Inoue, H., Shibuta, K., Tanaka, Y., Barnard, G. F., Sugimachi, K., and Mori, M.: "Hepatoma-derived growth factor is associated with reduced sensitivity to irradiation in esophageal cancer"Cancer Res. 61. 5714-7 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sadanaga, N., Nagashima, H., Mashino, K., Tahara, K., Yamaguchi, H., Ohta, M., Fujie, T., Tanaka, F., Inoue, H. Takesako, K., Akiyoshi, T., and Mori, M.: "Dendritic cell vaccination with MAGE peptide is a novel therapeutic approach for gastrointestinal carcinomas"Clin Cancer Res. 7. 2277-84 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mashino, K., Sadanaga, N., Tanaka, F., Yamaguchi, H., Nagashima, H., Inoue, H., Sugimachi, K., and Mori, M.: "Expression of multiple cancer-testis antigen genes in gastrointestinal and breast carcinomas"Br J Cancer. 85. 713-20 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mori, M., Mimori, K., Masuda, T., Yoshinaga, K., Yamashita, K., Matsuyama, A., and Inoue, H.: "Absence of Msh2 protein expression is associated with alteration in the FHIT locus and Fhit protein expression in colorectal carcinoma"Cancer Res. 61. 7379-82 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nagashima, H., Sadanaga, N., Mashino, K., Yamashita, K., Inoue, H., Mori, M., and Sugimachi, K.: "Expression of mage-b genes in esophageal squamous cell carcinoma"Jpn J Cancer Res. 92. 167-73 (2001)
Description
「研究成果報告書概要(欧文)」より
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