2001 Fiscal Year Annual Research Report

脳腫瘍における遺伝子発現機序に基づいた抗癌剤治療効果増強法の関発と臨床応用

Research Project

Project/Area Number	12470296
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	峯浦一喜京都府立医科大学, 医学部, 教授 (70134103)
Co-Investigator(Kenkyū-buntansha)	大和田敬京都府立医科大学, 医学部, 助手 (80332948) 橋本直哉京都府立医科大学, 医学部, 助手 (90315945) 笹島浩泰京都府立医科大学, 医学部, 講師 (80196188)
Keywords	脳腫瘍 / 化学療法 / アルオル化剤 / アルオル転移酵素 / 免疫染色 / 染色異常
Research Abstract	ヒト脳腫瘍の手術による摘出材料や生検材料について,O^6-methylguanine-DNA methyltransferase(MGMT)の多寡によるMer+/-診断法を検討した.脳腫瘍摘出標本において抗MGMT抗体による免疫染色法の定量標準化を試みた.42例のグリオーマを対象とし,MGMT陽性細胞を算定した.MGMT陽性細胞は1例を除いて陽性細胞が検出され,最高陽性率は66.7%で,平均は19.7%であった.陽性率が10%未満のものは10例(24%)に認められ,Mer-腫瘍に相当するものと考えられた. グリオーマのうちでオリゴデンドログリオーマの化学療法において,1番染色体短腕(1p)と19番染色体長腕(19q)の欠失例ではPCV(PCZ, CCNU(ACNU),VCR)併用化学療法が効果的である.オリゴデンドログリオーマ14例について,loss of heterozygosity(LOH)をFISH法で検討したところ,13例(93%)に1pの欠失,7例中6例(86%)に19qの欠失,1pと19qの両者の欠失は6例(86%)であり,オリゴデンドログリオーマでは1pおよび19p欠失が高率であることが判明した.

Research Products
(9 results)

All Other

All Publications (9 results)

[Publications] Fujino H, Imahori Y, Mineura K. et al.: "Non-invasive imaging of human Polomevase activity. Targe ting enzyme in BNCT."Proc of 9th International Symposium on Neuron Capture therapy. 327-328 (2000)
[Publications] Imahori Y, Mineura K, ChoK. et al.: "Boron Neutron Capture therapy using horono phenylalanine for high-grade glioma in Japan"Proc of 9th International Symposium on Neuron Capture therapy. 167-168 (2000)
[Publications] Takegami T, Ebisu T, Bito Y. et al.: "Mismatch between lactate and the apparent diffusion coefficient of water in progressive focal ischemia"NMR Biomed. 14. 5-11 (2001)
[Publications] Yamanaka T, Hashimoto N. et al.: "A case of diastemmyelia associated with myelesilnsis in a lwmicrod"Pecliatr Neurosurg. 35. 253-256 (2001)
[Publications] Sasajima H, Mineura K. et al.: "Surgery of deep-seated brain tumors"Proc of 2nd International Mt.Bandai Symposium. 126-130 (2001)
[Publications] Hashimoto N, Murakami M.dt al.: "Diagnosts molecular genetics and its application to clinical devision in brain tumor"Proc of 2nd International Mr.Bandai Symposium. 150-154 (2001)
[Publications] Hirata Masazumi: "Drug resistanc modification using pulaing electromagnetic field stimulation for multidrug resistant mouse osteosarcoma cell line"Anticancer Res. 21. 317-320 (2001)
[Publications] Kusuzaki katsuyuki: "Acridine orange induced binucleation in chondrocytes"Osteoarthritis Cartilage. 9. 147-151 (2001)
[Publications] Hashiguchi Shin: "Acridin orange excited by low-dose radiation has a strong cytocidal effect on mouse osteosarcoma"Oncology. 62. 85-93 (2002)

2001 Fiscal Year Annual Research Report

脳腫瘍における遺伝子発現機序に基づいた抗癌剤治療効果増強法の関発と臨床応用

Principal Investigator

峯浦 一喜 京都府立医科大学, 医学部, 教授 (70134103)

Research Products

[Publications] Fujino H, Imahori Y, Mineura K. et al.: "Non-invasive imaging of human Polomevase activity. Targe ting enzyme in BNCT."Proc of 9th International Symposium on Neuron Capture therapy. 327-328 (2000)

[Publications] Imahori Y, Mineura K, ChoK. et al.: "Boron Neutron Capture therapy using horono phenylalanine for high-grade glioma in Japan"Proc of 9th International Symposium on Neuron Capture therapy. 167-168 (2000)

[Publications] Takegami T, Ebisu T, Bito Y. et al.: "Mismatch between lactate and the apparent diffusion coefficient of water in progressive focal ischemia"NMR Biomed. 14. 5-11 (2001)

[Publications] Yamanaka T, Hashimoto N. et al.: "A case of diastemmyelia associated with myelesilnsis in a lwmicrod"Pecliatr Neurosurg. 35. 253-256 (2001)

[Publications] Sasajima H, Mineura K. et al.: "Surgery of deep-seated brain tumors"Proc of 2nd International Mt.Bandai Symposium. 126-130 (2001)

[Publications] Hashimoto N, Murakami M.dt al.: "Diagnosts molecular genetics and its application to clinical devision in brain tumor"Proc of 2nd International Mr.Bandai Symposium. 150-154 (2001)

[Publications] Hirata Masazumi: "Drug resistanc modification using pulaing electromagnetic field stimulation for multidrug resistant mouse osteosarcoma cell line"Anticancer Res. 21. 317-320 (2001)

[Publications] Kusuzaki katsuyuki: "Acridine orange induced binucleation in chondrocytes"Osteoarthritis Cartilage. 9. 147-151 (2001)

[Publications] Hashiguchi Shin: "Acridin orange excited by low-dose radiation has a strong cytocidal effect on mouse osteosarcoma"Oncology. 62. 85-93 (2002)

峯浦一喜京都府立医科大学, 医学部, 教授 (70134103)