2001 Fiscal Year Final Research Report Summary
Molecular biological Research on mechanisms of refinoic acid for dermal wound healing and treatment of hyper pigmentation
| Project/Area Number |
12470374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YOSHIMURA Kotaro U of Tokyo, Plastic Surgery, Assistant Professor, 医学部・附属病院, 講師 (60210762)
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| Co-Investigator(Kenkyū-buntansha) |
AOYAMA Takao U of Tokyo, Pharmacy, Associate, 医学部・附属病院, 助手 (60262028)
OKAZAKI Mutsumi U of Tokyo, Plastic Surgery, Associate, 医学部・附属病院, 助手 (50311618)
KITANO Yukie U of Tokyo, Plastic Surgery, Associate, 医学部・附属病院, 助手
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| Project Period (FY) |
2000 – 2001
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| Keywords | retinoic acid / HB-EGF / melanin / melanocyte / hydroguinone / retinol / retinal |
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| Research Abstract |
The results suggested that RA neither have direct inhibitory effects on melanogenesis of melanocytes, nor influence the cell-cell interactions between melanocytes, keratinocytes and fibroblasts, such as paracrine actions with regard to melanin productions. The role of RA in bleaching treatments appears to be other specific actions, such as promotion of keratinocytes proliferation and acceleration of epidermal turnover. The results also revealed that HB-EGF mRNA expression was elevated dose-dependently with atRA, most at 12 hours. This elevation was more prominent in keratinocytes of confluent state than those of subconfluent state, suggesting differentiated keratinocytes are more subject to be stimulated by atRA with regard to HB-EGF expression than proliferating keratinocytes. Ral and Rol was found to upregulate HB-EGF when used at 100 μM to 1mM, to a similar extent of atRA at 1 to 10 μM. The capacity of retinoids to upregulate HB-EGF may be an important index to develop and seek an ideal synthetic retinoid, which has maximum benefits and minimal side effects.
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