2001 Fiscal Year Final Research Report Summary
CD14-dependent and independent pathways in B cells activation after stimulation with lipopolysaccharides from periodontopathic bacteria
| Project/Area Number |
12470387
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
KIMURA Shigenobu Iwate Medical University School of Dentistry, Department of Oral Microbiology, Professor, 歯学部, 教授 (10177917)
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| Co-Investigator(Kenkyū-buntansha) |
TAJIKA Shihoko Iwate Medical University School of Dentistry, Department of Oral Microbiology, Instructor, 歯学部, 助手 (20048531)
NEMOTO Yuko (OHARA Yuko) Iwate Medical University School of Dentistry, Department of Oral Microbiology, Assistant Professor, 歯学部, 講師 (10164667)
SASAKI Minoru Iwate Medical University School of Dentistry, Department of Oral Microbiology, Associate Professor, 歯学部, 助教授 (40187133)
KOGA Toshiya Iwate Medical University School of Dentistry, Department of Oral Microbiology, Instructor, 歯学部, 助手 (10279309)
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| Project Period (FY) |
2000 – 2001
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| Keywords | periodontopathic bacteria / LPS / CD14 / B lymphocytes / signal transdution |
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| Research Abstract |
In this study we assessed the possible role of CD14 in B cells activation after stimulation with lipopolusaccharides from periodontopathic bacteria. We then examined the LPS-induced signal transduction mechanism that mediates later cellular responses of B cells. The results indicated that CD14 not restricted to myeloid dells, and was involved in some cellular activations and the isotype differentation to IgA-producing B cell to the enhanced expression of CDK TGF-β- production and the isotype different to IgA-producing B cells elicited by LPS. However, CD14-independent pathway could be also exist in the LPS-induced activation of B cells that eventually leads to proliferation, IL-6 production and the enhancement of IgM (but not IgA) secretion. It was also indicated that a trigger signal by the LPS from porphyromonas gingivalis as well as other periodontopathic bacteria, prevotella intermedia, Actinobacillus actinomycetemcomitans and Eikenella corrodens, could be transduced through both tyrosine and threonine phosphorylation pathways, and induced the activation of B cells that eventually leads to proliferation. Taken together, the present findings suggest to the LPSs from periodontopathic bacteria have a potent property to induce the stimulate of B cells through the CD14-dependent and independent activation pathways, in which increased tyrosine and threonine phosphorylation could be important signaling events that might lead to cellular responses.
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