2002 Fiscal Year Final Research Report Summary
Development of MrPDB, a full automatic software system of molecular replacement for protein
| Project/Area Number |
12480181
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
KUSUNOKI Masami Osaka University, Institute for Protein Research, Associate Professor, たんぱく質研究所, 助教授 (90135749)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Nobuo Tokyo Institute of Technology, Graduate School of Bioscience and Bitechnology, professor, 大学院・生命理工学研究科, 教授 (50032024)
KURISU Genji Osaka University, Institute for Protein Research, Research Associate, たんぱく質研究所, 助手 (90294131)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Molecular replacement / Protein Data Bank / Protein / crystal / X-ray / software |
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| Research Abstract |
Crystal structure of a protein can be determined by X-ray crystallography. Solved crystal structures of proteins have been deposited in the Protein Data Bank. If a crystal structure of a homologous protein with sequence identity greater than 30 % is already in the Protein Data Bank, then the crystal structure of the protein can be determined by the molecular replacement method, in which only native structure factor data of crystals are needed and hence the process of structure determination is straightforward.
We have developed Mr-PDB, a WEB-based automatic software system of molecular replacement for protein structure determination. The user input crystal data, amino-acid sequence in the WEB page of MrPDB and upload the file of crystal structure factors to the WEB server of MrPDB. Then MrMDP determines the crystalstructure of the protein automatically.
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Research Products
(8 results)
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[Publications] Kurisu, G., Kusunoki, M., Katoh, E., Yamazaki, T., Teshima, K., Onda, Y., Kimata-Ariga, Y., and Hase, T.: "Structure of the Electron Transfer Complex between Ferredoxin and Ferredoxin-NADP^+ Reductase"Nature Structural Biology. Vol.8, No.2. 117-121 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, K., Kurisu, G., Kusunoki, M., Tabata, S., Urabe, I., and S. Osaki, S.: "Crystal Structure of Glucose Dehydrogenase from Bacillus megaterium IWG3 at 1.7 A Resolution"Journal of Biochemistry. Vol.129, No.2. 303-312 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Otagiri, M., Kurisu, G., Ui, S., Takusagawa, Y., Ohkuma, M., Kudo, T. and Kusunoki, M.: "Crystal Structure of meso-2, 3-Butanediol Dehydrogenase in Complex with Inhibitor Mercaptoethanol at 1.7 A Resolution for Undertanding of Chiral Recognition Mechanismsfroms"Journal of Biochemistry. Vol.129, No.2. 205-208 (2001)
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「研究成果報告書概要(欧文)」より
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