2001 Fiscal Year Final Research Report Summary
Regulation of meiotic chromosome segregation by cohesin Rec8
Project/Area Number |
12480207
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
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Research Institution | The University of Tokyo |
Principal Investigator |
WATANABE Yoshinori Univ. of Tokyo, Grad. Sch. of Science, Associate Professor, 大学院・理学系研究科, 助教授 (20212326)
|
Project Period (FY) |
2000 – 2001
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Keywords | chromosome cohesion factor / centromere / heterochromatin / HP1 / Swi6 / equational division |
Research Abstract |
Fission yeast centromeres, like that of higher eukaryotes, are comprised of repeated DNA structures and associated heterochromatin protein complexes, that play a critical role in faithful segregation of chromosomes during cell division. Cohesin protein complexes, which are essential for sister chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats. We have identified functional and physical link, between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore, function. Cohesin is also enriched at the mating type heterochromatic region in a Swi6 dependent manner and is required to preserve genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homolog HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting proper segregation of chromosomes.
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Research Products
(12 results)
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[Publications] Kitamura, K, Katayama, S., Dhut, S., Sato, M., Watanabe, Y., Yamamoto, M., Toda, T.: "Phosphorylation of Mei2 and Ste11 by Pat1 Kinase Inhibits Sexual Differentiation via Ubiquitin Proteolysis and 14-3-3 Protein in Fission Yeast"Dev. Cell. 1. 389-399 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Mizuno Ki, K., Hasemi, T., Ubukata, T., Yamada., T., Lehmann, E., Kohli, J., Watanabe., Y., Iino, Y., Yamamoto, M., Fox, M.E., Smith, G.R., Murofushi, H., Shibata, T., Ohta, K.: "Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators"Genetics. 159. 1467-1478 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nonaka, N., Kitajima, T, Yokobayashi, S., Xiao, G., Yamamoto, M., Grewal, S., Watanabe, Y.: "Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast"Nature Cell Biol.. 4. 89-93 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kitamura, K., Katayama, S., Dhut, S., Sato, M., Watanabe, Y., Yamamoto, M., and Toda, T.: "Phosphorylation of Mei2 and Ste11 by Pat1 Kinase Inhibits Sexual Differentiation via Ubiquitin Proteolysis and 14-3-3 Protein in Fission Yeast"Dev. Cell. 1. 389-399 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mizuno Ki, K., Hasemi, T., Ubukata, T., Yamada, T., Lehmann, E., Kohli, J., Watanabe, Y., Iino, Y., Yamamoto, M., Fox, M. E., Smith, G. R., Murofushi, H., Shibata, T., and Ohta, K.: "Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators"Genetics. 159. 1467-1468 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nonaka, N., Kitajima, T., Yokobayashi, S., Xiao, G., Yamamoto, M., Grewal S., and Watanabe, Y.: "Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast"Nature Cell Biol.. 4. 89-93 (2002)
Description
「研究成果報告書概要(欧文)」より