Induction and maintenance mechanism of late phase of cerebellar synaptic plasticity

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12480238
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: HIRANO Tomoo Graduate School of Science, Department of Biophysics, Professor, 大学院・理学研究科, 教授 (50181178)
• Project Period (FY): 2000 – 2002
• Keywords: Cerebellum / Purkinje cell / Synaptic plasticity / Glutamate / GABA / miniature postsynaptic current / Transcription / Calcineurin

Research Abstract

The synaptic plasticity in the cerebellum has been considered as a cellular basis of motor learning. Long-term depression (LTD) at excitatory synapses between a granule neuron and a Purkinje neuron and rebound potentiation (RP) at inhibitory synapses between an interneuron and a Purkinje neuron, has been reported. The synaptic plasticity is composed of transcription-independent early phase and transcription-dependent late phase. In this study, we have studied the expression and maintenance mechanism of late phase of synaptic plasticity utilizing measurement of miniature excitatory or inhibitory postsynaptic current (mEPSC, mIPSC) in the rat cerebellar culture. We found that inhibition of calcineurin (a calcium and calmodulin dependent protein phosphatase) induced the late phase of LTD. Our results suggest that down regulation of calcineurin by superoxide triggers the induction of late phase of LTD in a Purkinje neuron. We also succeeded to demonstrate whole time course of RP. RP also consisted of early phase and transcription-dependent late phase lasting for more than a day.

Research Products

• [Publications] Fujii, H., Hirano, T.: "Calcineurin regulates induction of late phase of cerebellar long-term depression in rat cultured Purkinje neurons"European Journal of Neuroscience. 16. 1777-1788 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Minami, I. et al.: "Long-term potentiation of mEluRl activity by depolarization-induced homer1a in cerebellar Purkinje neurons"European Journal of Neuroscience. (印刷中). (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawaguchi, S. Hirano, T.: "Signaling cascade regulating long-term potentiation of GABA_A receptor responsiveness in cerebellar Purkinje neurons"Journal of Neuroscience. 22. 3969-3976 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hirano, T. et al.: "Roles of inhibitory interneurons in the cerebellar cortex"Annals of New York Academy of Science. 978. 405-412 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Eiraku, M. et al.: "Delta/Notch-like epidermal growth factor(EGF)-related receptor, a novel EGF-like repeat-containing protein targeted dendries of developing and adult central nervous system neurons"Journal of Biological Chemistry. 277. 25400-25407 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawaguchi, S., Hirano, T.: "Suppression of inhibitory synaptic potentiation by presynaptic activity through postsynaptic GABA_B receptors in a Purkinje neuron"Neuron. 27. 339-347 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fujii, H., Hirano, T.: "Calcineurin regulates induction of late phase of cerebellar long-term depression in rat cultured Purkinje neurons"Eur. J. Neurosci.. 16. 1777-1788 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kawaguchi, S., Hirano, T.: "Signaling cascade regulating long-term potentiation of GABA_A receptor responsiveness in cerebellar Purkinje neurons"J. Neurosci.. 22. 3969-3976 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Minami, L., Kengaku, M., Sillevis Smitt, P., Shigemoto, R., Hirano, T.: "Long-term potentiation of mGluRl activity by depolarization-induced Homerla in cerebellar Purkinje neurons"Eur. J. Neurosci.. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Eiraku, M., Hirata, Y., Takeshima, H., Hirano, T., Kengaku, M.: "Delta/Notch-like epidermal growth factor (EGF)-related receptor, a novel EGF-like repeat-containing protein targeted to dendrites of developing and adult central nervous system neurons"J. Biol. Chem.. 277. 25400-25407 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hirano, T., Watanabe, D., Kawaguchi, S., Pastan, I., Nakanishi, S.: "Roles of inhibitory interneurons in the cerebellar cortex"Annals of New York Academy of Science. 978. 405-412 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kawaguchi, S., Hirano, T.: "Suppression of inhibitory synaptic potentiation by presynaptic activity through postsynaptic GABA_B receptors in a Purkinje neuron"Neuron. 27. 339-347 (2000)
  • Description: 「研究成果報告書概要(欧文)」より