2002 Fiscal Year Annual Research Report

培養不能細菌の分子メカニズム

Research Project

Project/Area Number	12490009
Research Institution	The University of Tokyo
Principal Investigator	木暮一啓東京大学, 海洋研究所, 教授 (10161895)
Co-Investigator(Kenkyū-buntansha)	山本啓之聖マリアンナ医科大学, 医学部, 助教授 (30182645) 水之江義充九州大学, 大学院・医学系研究科, 助教授 (20157514) 千浦博国際基督教大学, 教養学部, 準教授 (00103698) 石浜明国立遺伝学研究所, 教授 (80019869)
Keywords	培養不能状態 / 細菌 / 酸素ストレス / ウイルス様粒子 / 化学合成生態系 / 密度勾配 / シグマファクター / 増殖期
Research Abstract	多くの細菌は、低栄養、低温などのストレス条件下におかれると、異なる生理状態に移行する。そうした状態のひとつとして、培養できないが、生理活性を維持した状態がある。本研究は、培養不能状態にある細菌の生理状態を、分子レベルで解明することを目的としている。その研究実績のいくつかを述べる。第一に、熱水や冷湧水が供給される深海底また温泉などの化学合成生態系は地球上での細菌の進化を考慮する上で鍵となる生態系である。これらの環境中の細菌群集の培養能を調べるとともに、その種組成を培養によらない方法を適用して調べた。第二に、海洋には細菌の10-100倍のウイルス様粒子が存在し、海洋細菌に感染することで、その死滅や培養能の喪失を招く。これらのウイルス様粒子を天然海水から濃縮、分離し、その機能、遺伝子伝播に与える影響について基礎的知見を得た。第三に、培養不能状態への移行あるいはそこからの復帰には活性酸素によるストレスが大きく関与していると予想されてきた。培養不能状態の菌に対してこうしたストレス状態が異なる条件を与え、実際にそれが培養能を左右していることを明らかにした。第四に、培養不能状態の菌は培養可能な菌や死滅した菌と共存していることにより、その解析が困難とされてきた。Percoll密度勾配法により、異なる培養能、遺伝子発現の状況下にある集団を分離、解析する新たな手法を開発した。第五に、大腸菌が対数増殖期から定常期に移行する際に、どのようなシグマファクターがどのような遺伝子群の転写に関与しているかについての基礎知見を得た。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] Makinoshima H, Aizawa SI, Hayashi H, Miki T, Nishimura A, Ishihama A: "Growth phase-coupled alterations in cell structure and function of Escherichia coli"Journal of Bacteriology. 185:(4). 1338-1345 (2003)
[Publications] Yamamoto K, Yata K, Fujita N, Ishihama A: "Novel mode of transcription regulation by SdiA, an Escherichia coli homologue of the quorum-sensing regulator"Molecular Microbiology. 41:(5). 1187-1198 (2001)
[Publications] Ghosh P, Ishihama A, Chatterji D: "Escherichia coli RNA polymerase subunit omega and its N-terminal domain bind full-length beta ' to facilitate incorporation into the alpha(2)beta subassembly"European Journal of Biochemistry. 268:(17). 4621-4627 (2001)
[Publications] Raj VS, Tomitori H, Yoshida M, Apirakaramwong A, Kashiwagi K, Takio K, Ishihama A, Igarashi K: "Properties of a revertant of Escherichia coli viable in the presence of spermidine accumulation : Increase in L-glycerol 3-phosphate"Journal of Bacteriology. 183:(15). 4493-4498 (2001)
[Publications] Makinoshima H, Nishimura A, Ishihama A: "Fractionation of Escherichia coli cell populations at different stages during growth transition to stationary phase"Molecular Microbiology. 43:(2). 269-279 (2002)
[Publications] Ishihama A: "Functional modulation of Escherichia coli RNA polymerase"Annual Revioew of Microbiology. 54. 499-518 (2000)
[Publications] Yoshida M, Kashiwagi K, Kawai G, Ishihama A, Igarashi K: "Polyamines enhance synthesis of the RNA polymerase sigma(38) subunit by suppression of an amber termination codon in the open reading frame"Journal of Biological Chemistry 277 : (40) 37139-37146 2002. 277:(40). 37139-37146 (2002)
[Publications] Culham DE, Lu A, Jishage M, Krogfelt KA, Ishihama A, Wood JM: "The osmotic stress response and virulence in pyelonephritis isolates of Escherichia coil : contributions of RpoS, Prop, ProU and other systems"Microbiology. 147. 1657-1670 (2001)
[Publications] Sujatha S, Ishihama A, Chatterji D: "Functional complementation between mutations at two distant positions in Escherichia coli RNA polymerase as revealed by second-site suppression"Sujatha S, Ishihama A, Chatterji D Molecular and General Genetics. 264:(5). 531-538 (2001)
[Publications] Azam TA, Hiraga S, Ishihama A: "Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid"Genes to Cells. 5:(8). 613-626 (2002)
[Publications] Fujita N, Endo S, Ishihama A: "Structural requirements for the interdomain linker of alpha subunit of Escherichia coli RNA polymerase"Biochemistry. 39:(20). 6243-6249 (2002)
[Publications] Kimura M, Ishihama A: "Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II"Nucleic Acid Research 28. 28:(4). 952-959 (2000)
[Publications] Ozoline ON, Fujita N, Ishihama A: "Transcription activation mediated by the carboxyl-terminal domain of the RNA polymerase alpha-subunit -Multipoint monitoring using a fluorescent probe"Journal of Biological Chemistry. 245:61-67 2002. 61-67 (2002)
[Publications] Hiroyuki Yamamoto, Katsunori Fujikura, Akira Hiraishi, Kenji Kato, Yonosuke Maki: "Phylogenetic characterization and biomass estimation of bacterial endosymbionts associated with invertebrates dwelling in chemosynthetic communites of hydrothermal vent and cold seep fields"Marine Ecology Progress Series. 245. 61-67 (2002)
[Publications] Nishino, T., B.B.Nayak, K.Kogure: "Density dependent sorting of physiologically different cells of Vibrio parahaemolyticus"Applied and Environmental Microbiology. (in press).
[Publications] Nagata, Erina, Kazuhiro Kogure, Kumiko Kita-Tsukamoto, Minoru Wada, Mitsuru Eguchi: "Na+ dependent respiratory chain in Vibrio anguillarum, a fish pathogen, isolated from a freshwater area"FEMS Microbial Ecology. (in press).
[Publications] Ishikawa T, Mizunoe Y, Kawabata S, Takade A, Harada M, Wai SN, Yoshida S: "The iron-binding protein Dps confers hydrogen peroxide stress resistance to Campylobacter jejuni"JOURNAL OF BACTERIOLOGY. 185:(3). 1010-1017 (2003)
[Publications] Saito M, Ohga S, Endoh M, Nakayama H, Mizunoe Y, Hara T, Yoshida S: "H2O2-nonproducing Streptococcus pyogenes strains : survival in stationary phase and virulence in chronic granulomatous disease"MICROBIOLOGY-SGM. 147. 2469-2477 (2001)
[Publications] Wai SN, Mizunoe Y, Takade A, Yoshida S: "A comparison of solid and liquid media for resuscitation of starvation-and low-temperature-induced nonculturable cells of Aeromonas hydrophila"ARCHIVES OF MICROBIOLOGY. 173:(4). 307-310 (2000)
[Publications] Mizunoe Y, Wai SN, Ishikawa T, Takade A, Yoshida S: "Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation"FEMS MICROBIOLOGY LETTERS. 186:(1). 115-120 (2000)
[Publications] Hiroshi Xavier Chiura: "Broad Host Range Xenotrophic Gene Transfer by Virus-Like Particles from a Hot Spring"Microbes and Environments. 17(2002). 53-58 (2002)
[Publications] Hiroshi Xavier Chiura, Hiroyuki Yamamoto, Daisuke Koketsu, Hiroki Naito, Kenji Kato: "Virus-Like Particle derived from a Bacterium belonging to the Oldest Lineage of the Domain Bacteria"Microbes and Environments. 17. 48-52 (2002)
[Publications] Satoru Suzuki, Shin-ichi Kitamura, Hiroshi X.Chiura: "Aquabirnavirus Widely Distributes in the Ocean, Providing a Dissolved RNA Pool"Microbes and Environments. 16. 191-196 (2001)

2002 Fiscal Year Annual Research Report

培養不能細菌の分子メカニズム

Principal Investigator

木暮 一啓 東京大学, 海洋研究所, 教授 (10161895)

Research Products

[Publications] Makinoshima H, Aizawa SI, Hayashi H, Miki T, Nishimura A, Ishihama A: "Growth phase-coupled alterations in cell structure and function of Escherichia coli"Journal of Bacteriology. 185:(4). 1338-1345 (2003)

[Publications] Yamamoto K, Yata K, Fujita N, Ishihama A: "Novel mode of transcription regulation by SdiA, an Escherichia coli homologue of the quorum-sensing regulator"Molecular Microbiology. 41:(5). 1187-1198 (2001)

[Publications] Ghosh P, Ishihama A, Chatterji D: "Escherichia coli RNA polymerase subunit omega and its N-terminal domain bind full-length beta ' to facilitate incorporation into the alpha(2)beta subassembly"European Journal of Biochemistry. 268:(17). 4621-4627 (2001)

[Publications] Raj VS, Tomitori H, Yoshida M, Apirakaramwong A, Kashiwagi K, Takio K, Ishihama A, Igarashi K: "Properties of a revertant of Escherichia coli viable in the presence of spermidine accumulation : Increase in L-glycerol 3-phosphate"Journal of Bacteriology. 183:(15). 4493-4498 (2001)

[Publications] Makinoshima H, Nishimura A, Ishihama A: "Fractionation of Escherichia coli cell populations at different stages during growth transition to stationary phase"Molecular Microbiology. 43:(2). 269-279 (2002)

[Publications] Ishihama A: "Functional modulation of Escherichia coli RNA polymerase"Annual Revioew of Microbiology. 54. 499-518 (2000)

[Publications] Culham DE, Lu A, Jishage M, Krogfelt KA, Ishihama A, Wood JM: "The osmotic stress response and virulence in pyelonephritis isolates of Escherichia coil : contributions of RpoS, Prop, ProU and other systems"Microbiology. 147. 1657-1670 (2001)

[Publications] Sujatha S, Ishihama A, Chatterji D: "Functional complementation between mutations at two distant positions in Escherichia coli RNA polymerase as revealed by second-site suppression"Sujatha S, Ishihama A, Chatterji D Molecular and General Genetics. 264:(5). 531-538 (2001)

[Publications] Azam TA, Hiraga S, Ishihama A: "Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid"Genes to Cells. 5:(8). 613-626 (2002)

[Publications] Fujita N, Endo S, Ishihama A: "Structural requirements for the interdomain linker of alpha subunit of Escherichia coli RNA polymerase"Biochemistry. 39:(20). 6243-6249 (2002)

[Publications] Kimura M, Ishihama A: "Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II"Nucleic Acid Research 28. 28:(4). 952-959 (2000)

[Publications] Ozoline ON, Fujita N, Ishihama A: "Transcription activation mediated by the carboxyl-terminal domain of the RNA polymerase alpha-subunit -Multipoint monitoring using a fluorescent probe"Journal of Biological Chemistry. 245:61-67 2002. 61-67 (2002)

[Publications] Nishino, T., B.B.Nayak, K.Kogure: "Density dependent sorting of physiologically different cells of Vibrio parahaemolyticus"Applied and Environmental Microbiology. (in press).

[Publications] Nagata, Erina, Kazuhiro Kogure, Kumiko Kita-Tsukamoto, Minoru Wada, Mitsuru Eguchi: "Na+ dependent respiratory chain in Vibrio anguillarum, a fish pathogen, isolated from a freshwater area"FEMS Microbial Ecology. (in press).

[Publications] Ishikawa T, Mizunoe Y, Kawabata S, Takade A, Harada M, Wai SN, Yoshida S: "The iron-binding protein Dps confers hydrogen peroxide stress resistance to Campylobacter jejuni"JOURNAL OF BACTERIOLOGY. 185:(3). 1010-1017 (2003)

[Publications] Saito M, Ohga S, Endoh M, Nakayama H, Mizunoe Y, Hara T, Yoshida S: "H2O2-nonproducing Streptococcus pyogenes strains : survival in stationary phase and virulence in chronic granulomatous disease"MICROBIOLOGY-SGM. 147. 2469-2477 (2001)

[Publications] Wai SN, Mizunoe Y, Takade A, Yoshida S: "A comparison of solid and liquid media for resuscitation of starvation-and low-temperature-induced nonculturable cells of Aeromonas hydrophila"ARCHIVES OF MICROBIOLOGY. 173:(4). 307-310 (2000)

[Publications] Mizunoe Y, Wai SN, Ishikawa T, Takade A, Yoshida S: "Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation"FEMS MICROBIOLOGY LETTERS. 186:(1). 115-120 (2000)

[Publications] Hiroshi Xavier Chiura: "Broad Host Range Xenotrophic Gene Transfer by Virus-Like Particles from a Hot Spring"Microbes and Environments. 17(2002). 53-58 (2002)

[Publications] Hiroshi Xavier Chiura, Hiroyuki Yamamoto, Daisuke Koketsu, Hiroki Naito, Kenji Kato: "Virus-Like Particle derived from a Bacterium belonging to the Oldest Lineage of the Domain Bacteria"Microbes and Environments. 17. 48-52 (2002)

[Publications] Satoru Suzuki, Shin-ichi Kitamura, Hiroshi X.Chiura: "Aquabirnavirus Widely Distributes in the Ocean, Providing a Dissolved RNA Pool"Microbes and Environments. 16. 191-196 (2001)

木暮一啓東京大学, 海洋研究所, 教授 (10161895)