Analysis of Genome Operating Systems for Immune Network by Polycomb Group Genes.

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12490025
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 広領域
• Research Institution: HIROSHIMA UNIVERSITY
• Principal Investigator: KANNO Masamoto Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (40161393)
• Co-Investigator(Kenkyū-buntansha): INOUE Hiroko Hiroshima University, Faculty of Medicine, Teaching Associate, 医学部, 教務員 (90136060) ; NINOMIYA Yuichi Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (70334175)
• Project Period (FY): 2000 – 2002
• Keywords: Thymus / T-lymphocyte / immature diffarentitation / Folycomb group gene / Protein complex / Chromatin

Research Abstract

The Polycomb group (PcG) genes products form protein complexes in mammalian cell nuclei that contribute to maintain chromatin silencing of target genes. Among mammalian PcG homologues, mel-18 has been elucidated to regulate cell cycle progression, cell death, or senescence through analyses of knockout/Tg mice. Its product participatesin Polycomb protein complexes, whose existences in cell nuclei are displayed speckled distributions, overlapped with heterochromatin areas in immunohistochemical examinations.
We describe that the defect in thymic T-lymphocyte development of mel-18 deficient mice implies the involvement of mel-18 in the maintenance of immature lymphocyte by regulating their survival via controlling the expression of some commitment and survival factors including subgroup of bcl-2 family. Therefore we would like to propose our results as the regulation mechanisms to control the balance between proliferation and cell death in lymphocyte development, especially ETP cell of DN1 fraction and small cell (E cell) of DN3 fraction just before the beta-selection of immature thymocyte by destabilization of chromatin silencing Polycomb protein complex.

Research Products

• [Publications] Muto A.: "Activation of Maf/AP-1 Repressor Bach2 by Oxidative Stress Promotes Apoptosis and Its Interaction with Promyelocytic Leukemia Nuclear Bodies"J Biol Chem. 277. 20724-20733 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyazaki, K.: "Chemokine-mediated thymopoiesis is regulated by a mammalian Polycomb group gene, mel-18"Immunol Lett.. 80. 139-143 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fujisaki S: "Dimerization of the Polycomb-group protein Mel-18 is regulated by PKC phosphorylation"BBRC. 300. 135-140 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakai R: "TCDD treatment eliminates the long-term reconstitution activity of hematopoietic stem cells"Toxicol.Sci.. 72. 84-91 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyazaki , K., Inoue , H., Onai , N., Ishihara , H., and Kanno, M.: "Chemokine-mediated thymopoiesis is regulated by a mammalian Polycomb group gene, mel-18."Immunol Lett.. 80. 139-143 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Muto A, Tashiro S, Tsuchiya H, Kume A, Kanno M, Ito E, Yamamoto M, Igarashi K.: "Activation of Maf/AP-1 Represser Bach2 by Oxidative Stress Promotes Apoptosis and Its Interaction with Promyelocytic Leukemia Nuclear Bodies."J Biol Chem.. 277. 20724-20733 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fujisaki S, Ninomiya Y, Ishihara H, Miyazaki M, Kanno R, Asahara T, and Kanno M.: "Dimerization of the Polycomb-group protein Mel-18 is regulated by PKC phosphoryjation."BBRC. 300. 135-140 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sakai R, Kajiume T, Inoue H, Kanno R, Miyazaki M, Ninomiya Y, and Kanno M.: "TCDD treatment eliminates the long-term reconstitution activity of hematopoietic stem cells."Toxicol. Sci.. 72. 84-91 (2003)
  • Description: 「研究成果報告書概要(欧文)」より