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2001 Fiscal Year Final Research Report Summary

Design of Artificial Cell Membrane Interface and Development of Evaluation System for Biorecognition Function

Research Project

Project/Area Number 12554029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 分離・精製・検出法
Research InstitutionTohoku University

Principal Investigator

HAYASHITA Takashi  Tohoku University, Dept. of Chem., Associate Professor, 大学院・理学研究科, 助教授 (70183564)

Co-Investigator(Kenkyū-buntansha) NISHIZAWA Seiichi  Tohoku University, Dept. of Chem., Research Associate, 大学院・理学研究科, 助手 (40281969)
UCHIDA Tatsuya  Tohoku University, Dept. of Chem., Research Associate, 大学院・理学研究科, 助手 (30261548)
Project Period (FY) 2000 – 2001
KeywordsArtificial cell / Micelle / Vesicle / Thiourea probe / Crown ether probe / Anion recognition / Alkali metal ion recognition / Second harmonic generation
Research Abstract

To develop an anion sensing system in water based on a chromoionophore/artificial cell membrane conjugate, novel thiourea-based chromoionophores having various lengths of alkyl chains (Cn-TU) were designed and their anion recognition function in aqueous vesicle solutions were evaluated. This study revealed that the positioning of chromophore binding sites inside the didodecyldimethylammonium bromide (DDAB) vesicle was successfully controlled by the alkyl chain length of Cn-TU. The cationic vesicle interface was found to exhibit a filter function for the less hydrophobic anions, and highly selective anion recognition via hydrogen bonding was achieved by C1-TU located deep inside the DDAB vesicle. Function of crown ether chromoionophores for alkali metal ion recognition in micellar system were also examined. The surface charge of the micelle was found to strongly influence the ion selectivity and sensitivity, and selective Na^+ recognition was only performed by anionic micellar system. To evaluate ion recognition at these membrane interfaces, a resonant second harmonic generation (SHG) analysis system was developed by using 15-crown-5 azophenol as a model probe. Selective sorption of alkali metal complexes with the azoprobe at heptane/water interface was confirmed by SHG spectra, and their conformation changes upon metal recognition at the interface were successfully evaluated by SHG analysis.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] T.Hayashita, T.Onodera, R.Kato, N.Teramae et al.: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Vesicle Solution"Chem.Commun.. 755-756 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山内晶世, 早下隆士: "光情報変換に基づく金属イオン認識プローブの設計と機能"Bunseki Kagaku. 49(2). 75-89 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Kato, S.Nishizawa, T.Hayashita, N.Teramae: "A Thiourea-Based Chromoionophore for Selective Binding and Sensing of Acetate"Tetrahedron Letter. 42. 5053-5056 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 早下隆士, 加藤 亮, 小野寺恒信, 西沢精一, 寺前紀夫: "チオ尿素型比色プローブ/ベシクル複合体センサーの設計と機能評価"東北大学大学院理学研究科附属化学 機器分析センター年報. 1. 5-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Hayashita, T. Onodera, R. Kato, S. Nishizawa, N. Teramae: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Vesicle Solution"Chem. Commun.. 755-756 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Yamauchi, T. Hayashita: "Design and Function of Metal-ion Probes Based on Photosignal Transduction"Bunseki Kagaku. 49 (2). 75-89 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Kato, S. Nishizawa, T. Hayashita, N. Teramae: "A Thiourea-Based Chromoionophore for Selective Binding and Sensing of Acetate"Tetrahedron Letter. 42. 5053-5056 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Hayashita, R. Kato, T. Onodera, S. Nishizawa, N. Teramae: "Design and Function of Thiourea Chromoionophore/Vesicle Complex Sensor"Annual Report for Instrumental Analysis Center for Chemistry, Graduate School of Science, Tohoku University. 1. 5-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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