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2001 Fiscal Year Final Research Report Summary

Molecular breeding for submergence tolerance in crops

Research Project

Project/Area Number 12556002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

HIRAI Atsushi  Agric. Life Sci., THE UNIVERSITY OF TOKYO, Professor, 大学院・農学生命科学研究科, 教授 (60023470)

Co-Investigator(Kenkyū-buntansha) YOSHIBA Yoshu  Hitachi, Ltd. Central Res. Lab. Senior Res. Sct., 中央研究所, 主任研究員
NAKAZONO Mikio  Agric. Life Sci., THE UNIVERSITY OF TOKYO, Assit. Professor, 大学院・農学生命科学研究科, 助手 (70282697)
Project Period (FY) 2000 – 2001
KeywordsAcetaldehyde / Aldehyde dehydrogenase / Submergence / Rice
Research Abstract

It is known that alcoholic fermentation is important for survival of plants under anaerobic conditions. Acetaldehyde, one of the intermediates of alcoholic fermentation, is not only reduced by alcohol dehydrogenase (ADH) but also can be oxidized by aldehyde dehydrogenase (ALDH). To determine whether ALDH plays a role in anaerobic metabolism in rice (Oryza sativa L.), we characterized a cDNA clone encoding mitochondrial ALDH from rice (Aldh2a). Analysis of subcellular localization of ALDH2a protein and an in vitro ALDH assay indicated that ALDH2a functions in the oxidation of acetaldehyde in mitochondria. A Southern blot analysis indicated that mitochondrial ALDH is encoded by at least two genes in rice. We found that the Aldh2a mRNA was present at high levels in leaves of dark-grown seedlings, mature leaf sheaths and panicles. Interestingly, expression of the rice Aldh2a gene, unlike the expression of the tobacco Aldh2a gene, was induced in rice seedlings by submergence. A possible involvement of ALDH2a in the submergence tolerance of rice was studied.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Nakazono, M. et al.: "Characterization of two cDNA clones encoding rice FiFo-ATPase inhibitor proteins, both of which are targeted to mitochondria"Planta. 210. 188-194 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji, H. et al.: "Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the alternative oxidase 1a gene"FEBS Lett.. 471. 201-204 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li, Y. et al.: "Molecular and cellular characterizations of a cDNA clone encoding a novel isozyme of aldehyde dehydrogenase from rice"Gene. 249. 67-74 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazono, M. et al.: "Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions"Plant Physiol.. 124. 587-598 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohtsu, K. et al.: "Characterization and expression of the genes for cytochrome c oxidase subunit VIb (COX6b) from rice and Arabidopsis thaliana"Gene. 264. 233-239 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe, A., A.Hirai: "Two uncoupling protein genes of rice (Oryza sativa L.) : molecular study reveals the defects in the pre-mRNA processing for the heat-generating proteins of the subtropical cereal"Planta. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazono, M., T. Imamura, N. Tsutsumi, T. Sasaki and A. Hirai: "Characterization of two cDNA clones encoding rice F_1F_0-ATPase inhibitor proteins, both of which are targeted to mitochondria"Planta. 210. 188-194 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuji, H., M. Nakazono, D. Saisho, N. Tsutsumi and A. Hirai: "Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation : evidence for involvement of calcium in expression of the alternative oxidase 1a gene"FEBS Lett.. 471. 201-204 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li, Y., M. Nakazono, N. Tsutsumi and A. Hirai: "Molecular and cellular characterizations of a cDNA clone encoding a novel isozyme of aldehyde dehydrogenase from rice"Gene. 249. 67-74 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakazono, M., H. Tsuji, Y. Li, D. Saisho, S. Arimura, N. Tsutsumi and A. Hirai: "Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions"Plant Physiol.. 124. 587-598 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohtsu, K., M. Nakazono, N. Tsutsumi and A. Hirai: "Characterization and expression of the genes for cytochrome c oxidase subunit VIb (COX6b) from rice and Arabidopsis thaliana"Gene. 264. 233-239 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, A, and A. Hirai: "Two uncoupling protein genes of rice (Oryza sativa L.) : molecular study reveals the defects in the pre-mRNA processing for the heat-generating proteins of the subtropical cereal"Planta. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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