2003 Fiscal Year Final Research Report Summary
PROPAGATION AND FIELD TRIAL OF TRANSGENIC PERSIMMON ROOTSTOCKS WITH THE GENE FOR COMPATIBLE SOLUTE BIOSYNTHESIS
| Project/Area Number |
12556005
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
園芸・造園学
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
TAO Ryutaro KYOTO UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学研究科, 助教授 (10211997)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANAYAMA Yoshinori TOHOKU UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURAL SCIENCE, ASSOCIATE PROFESSOR, 農学研究科, 助教授 (10233868)
TETSUMURA Takuya MIYAZAHI UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (00227498)
HABU Tsuyoshi KYOTO UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, INSTRUCTOR, 農学研究科, 助手 (60335304)
|
|---|
| Project Period (FY) |
2000 – 2003
|
| Keywords | GLYCINE BETAINE / SORBITOL / CUTTINGS / AGROBACTERIUM |
|---|
| Research Abstract |
Many organisms, including certain plants, accumulate water-soluble organic compounds of low molecular weight that have no inhibitory effects on metabolism. These compounds are referred to as compatible solutes and allow them to tolerate certain types of environmental stress not only by adjusting osmotic pressure in cells but also by stabilizing the quaternary structures of complex proteins. Recent studies have shown that enhancement of tolerance to environmental stresses in plants can be achieved by genetic engineering of the biosynthesis of compatible solutes. All these studies, however, deal with herbaceous plant species. We selected Japanese persimmon (Diospyros kaki), which is one of the major fruit crops in Japan and susceptible to damage from salt and drought stresses, as a candidate for the first woody plant species to be genetically engineered for tolerance to environmental stress by transforming the genes involved in compatible solutes. Chimeric genes constructed from the choline oxidase gene of Arhrobacter globiformis and the cDNA for sorbitol-6-phosphate dehydrogenase of apple (Malus x domestica) were integrated into the persimmon genome to confer the ability to produce glycinebetaine and sorbitol, respectively. Tolerance to salt stress of transformed persimmon plants were determined by measuring the ratio of the variable (Fv) to the maximum (Fm) fluorescence of chlorophyll in leaves under NaCl stress. The rate of decline in Fv/Fm under NaCl stress was lower in transgenic persimmon plants producing glycinebetaine or sorbitol than control transformed and non-transformed lines. The possibility of using these transgenic persimmon as a rootstock was tested.
|
