2002 Fiscal Year Final Research Report Summary
Development of hyper-spectral imaging system for diagnosis of growth and photosynthetic reactions
| Project/Area Number |
12556041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物環境
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
OMASA Kenji Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (70109908)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Hidenori Ehime University, Faculty of Science, Professor, 理学部, 教授 (60124682)
OKI Kazuo Grad. School of Agricultural and Life Sciences, Lecturer, 大学院・農学生命科学研究科, 講師 (50292628)
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| Project Period (FY) |
2000 – 2002
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| Keywords | plant growth / fluorescence spectrophotometer / chlorophyll content / NPQ / PSII vield / herbicide / water stress / 水ストレス |
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| Research Abstract |
In this research, hyper-spectral imaging systems for diagnosis of growth and photosynthesis were developed. Growth and chlorophyll contents of wheat plants growing in field areas with different fertilizer application were estimated by the band ratio of 550 nm and 900 nm images measured using this system. Meanwhile, a fluorescence spectrometry is used to non-destructively and remotely obtain information on pigments, cell structure, physiological functions of plant leaves. Excitation-emission matrices of water-stressed spinach leaves in the dark were measured by a 3-D fluorescence spectrophotometer, and changes with time of these matrices were analyzed. F450/F680 and F450/F725 excited by short-wavelength ultraviolet rays (for example, 330nm) were suitable to detect changes of relative water content in spinach leaves. Using a developed chlorophyll (Chl) fluorescence imaging system, the photosynthetic effects of a commercially available soil-application type urea herbicide on attached Cucumis sativus L. leaves were analyzed. The inhibitions of primary electron transport in injured areas were detected from Chl fluorescence induction (CFI) images (Changes in intensity of Chl fluorescence during dark-light transition) taken by the CFI method. NPQ (non-photochemical quenching) and Yield (photosystem II photochemical efficiency) images were calculated from fluorescence images taken by the saturation pulse method. NPQ was reduced greatly in injured areas and NPQ images were useful to quantify the photosynthetic injuries. However, Yield was not reduced as much as the decrease in assimilation rate. This showed that Yield images were not suitable for diagnosis of the photosynthetic injuries of attached plant leaves caused by the herbicide.
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