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2002 Fiscal Year Final Research Report Summary

Development of Technology for Regulating Reproductive Efficiency by Controlling Corpus Luteum Function in Cows

Research Project

Project/Area Number 12556046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied animal science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

MIYAMOTO Akio  Obihiro University of Agriculture and Veterinary Medicine, Department of Agricultural and Life Science, Associate Professor, 畜産学部, 助教授 (10192767)

Co-Investigator(Kenkyū-buntansha) OHTANI Masayuki  Obihiro University of Agriculture and Veterinary Medicine, Field Center of Animal Science and Agriculture, Assistant Professor, 畜産学部, 講師 (80250538)
OKUDA Kiyoshi  Okayama University, Faculty of Agriculture, Professor, 大学院・自然科学研究科, 教授 (40177168)
Project Period (FY) 2000 – 2002
KeywordsCorpus luteum / Angiogenesis / Prostaglandin / Vasoactive peptide / Blood flow / Progesterone / hCG / Cow
Research Abstract

The ovarian function is controlled by vascular functions in various local phenomena including ovulation, corpus luteum (CL) development and regression. This study was conducted to develop a basic as well as applicable technology to activate CL angiogenesis and function thereby improving the conception rate by keeping high progesterone level in the cow. At the same time, a local secretion by a microdialysis system together with a local blood flow by a color Doppler ultrasonography observation of ovulatory follicle and CL during development and regression was examined in detail in vivo. From the series of experiments, the following points were clarified for the first time.
1. A LH surge activates a complex of local secretion of PG and vasoactive peptides that induces an acute increase in the local blood flow within the preovulatory follicular wall, but not within the atretic follicles. These changes are essential process to ovulation. Consequently, we proposed the existence of endothelin- … More angiotensin-ANP system in the preovulatory follicles.
2. The active angiogenesis occurring immediately after ovulation directly relates to the formation of CL and its function (progesterone secretion), and this local mechanism involves secretory function of PG, angiotensin and ANP, but not for endothelin. Particularly, a luteolytic factor PGF2α produced in the developing CL play a role as a luteotropic rather than luteolytic factor. This unique mechanism may directly correlate with anti-luteolytic characteristic of developing CL.
3. Once the CL matures, PGF2α can induce luteolysis in which other vasoactive peptides such as endothelin and angiotensin are highly active and luteolytic. This local mechanism induces a serious inhibition of local blood flow within the CL so that a cascade of luteolysis is accelerated further.
4. A single injection of hCG to the cow one day after ovulation effectively activates the CL formation as well as plasma progesterone level, possible by promoting angiogenesis in the CL. To confirm the impact of this treatment on the conception rate after artificial insemination and embryo transfer, the field studies are also on going. Less

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Kobayashi S, Acosta TJ, Ozawa T, Hayashi K, Berisha B, Ohtani M, Schams D, Miyamoto A: "Intraluteal release of angiotensin II and progesterone in vivo during corpora lutea development in the cow : effect of vasoactive peptides"Biology of Reproduction. 66. 174-179 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Acosta TJ, Yoshizawa N, Ohtani M, Miyamoto A: "Local changes in the blood flow within the early and midcycle corpus luteum after prostaglandin F2α injection in the cow"Biology of Reproduction. 66. 651-658 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi K, Tanaka J, Hayashi KG, Hayashi M, Miyamoto A: "The cooperative action of angiotensin II with subluteolytic administration of PGF2α in inducing luteolysis and the estrus in the cow"Reproduction. 124. 311-315 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Berisha B, Schams D, Miyamoto A: "The mRNA expression of angiotensin and endothelin system members in bovine ovarian follicles during final follicular growth"Journal of Reproduction and Development. 48. 553-560 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi S, Acosta TJ, Hayashi K, Berisha B, Ozawa T, Ohtani M, Schams D, Miyamoto A: "Intraluteal release of prostaglandin F2α and E2 during corpora lutea development in the cow"Journal of Reproduction and Development. 48. 583-590 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Berisha B, Schams D, Miyamoto A: "The expression of angiotensin and endothelin system members in bovine corpus luteun during estrous cycle and pregnancy"Endocrine. 19. 305-312 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi K, Miyamoto A, Konari A, Ohtani M, Fukui Y: "Effect of local interaction of reactive oxygen species with prostaglandin F2α on the release of progesterone in ovine corpora lutea in vivo"Theriogenology. 59. 1335-1344 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi K, Acosta TJ, Bersha B, Kobayashi S, Ohtani M, Schams D, Miyamoto A: "Changes in prostaglandin secretion by the regressing bovine corpus luteum"Prostaglandins & Other Lipid Mediators. 70. 339-349 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi K, Tanaka J, Hayashi KG, Hayashi M, Miyamoto A: "The cooperative action of angiotensin II with subluteolytic administration of PGF2α in inducing luteolysis and the estrus in the cow"Reproduction. 124. 311-315 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Acosta TJ, Hayashi KG, Ohtani M, Schams D, Miyamoto A: "Local changes in blood flow within the preovulatory follicle wall and early corpus luteum in cows"Reproduction. 125(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuka M, Yamamoto S, Hayashida N, Hayashi KG, Hayashi M, Acosta TJ, Miyamoto A: "Expression of 11β-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum"Journal of Endocrinology. 148(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Acosta TJ, Miyamoto A: "Animal Frontier Sciences"Hokuto Shobo, Kyoto Japan. 363 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi K: "Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum"Biology of Reproduction. 62. 162-167

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozawa T: "Immunoreactivity for gonadotropin-releasing hormone in microdialyzed perfusates of bovine mature follicles in vitro"Journal of Reproduction and Development. 46. 109-114

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukui Y: "Effect of bovine somatotropin administration on Day 11 after artificial insemination in estrus-induced ewes during the non-breeding season"Journal of Reproduction and Development. 46. 133-138

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Levy N: "Administration of prostaglandin F_<2α> during the early bovine luteal phase does not alter the expression of ET-1 and of its type A receptor: A possible cause for corpus luteum refractoriness"Biology of Reproduction. 63. 377-382

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi S: "Prostaglandin F_<2α> increases lipoprotein utilization for progesterone in bovine early corpora lutea in vitro"Journal of Reproduction and Development. 46. 335-339

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Acosta TJ: "Periovulatory changes in the local release of vasoactive peptides, prostaglandin F_<2α> and steroid hormones from bovine mature follicles in vivo"Biology of Reproduction. 63. 1253-1261

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi S: "Growth hormone, but not luteinizing hormone, acts with luteal peptides on prostaglandin F_<2α> and progesterone secretion by bovine corpora lutea in vitro"Prostaglandins & Other Lipid Mediators. 63. 79-92

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohtani M: "Changes of progesterone and endothelin concentrations in the peripheral plasma of female calves and cycling cows: Effects of PGF_<2α> injection"Journal of Reproduction and Development. 47. 37-43

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Schams D: "Stimulatory and synergistic effects of luteinising hormone and insulin-like growth factor 1 on the secretion of vascular endothelial growth factor and progesterone of cultured bovine granulose cells"Experimental and Clinical Endocrinology & Diabetes. 109. 155-162

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi S: "Production and localization of angiotensin II in the bovine early corpus luteum: a possible interaction with luteal angiogenic factors and prostaglandin F_<2α>"Journal of Endocrinology. 170. 369-380

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuda K: "Estradiol-17β is produced in bovine corpus luteum"Biology of Reproduction. 65. 1634-1639

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi S: "Intraluteal release of angiotensin II and progesterone in vivo during corpora lutea development in the cow: effect of vasoactive peptides"Biology of Reproduction. 66. 174-179

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Acosta TJ: "Local changes in the blood flow within the early and midcycle corpus luteum after prostaglandin F_<2α> injection in the cow"Biology of Reproduction. 66. 651-658

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi K: "The cooperative action of angiotensin II with subluteolytic administration of PGF_<2α> in inducing luteolysis and the estrus in the cow"Reproduction. 124. 311-315

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Berisha B: "The mRNA expression of angiotensin and endothelin system members in bovine ovarian follicles during final follicular growth"Journal of Reproduction and Development. 48. 553-560

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi S: "Intraluteal release of prostaglandin F_<2α> and E_2 during corpora lutea development in the cow"Journal of Reproduction and Development. 48. 583-590

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Berisha B: "The expression of angiotensin and endothelin system members in bovine corpus luteum during estrous cycle and pregnancy"Endocrine. 19. 305-312

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi K: "Effect of local interaction of reactive oxygen species with prostaglandin F_<2α> on the release of progesterone in ovine corpora lutea in vivo"Theriogenology. 59. 1335-1344

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi K: "Changes in prostaglandin secretion by the regressing bovine corpus luteum"Prostaglandins & Other Lipid Mediators. 70. 339-349

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Acosta TJ: "Local changes in blood flow within the preovulatory follicle wall and early corpus luteum in cows"Reproduction. 125 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuka M: "Expression of 11β-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum"Journal of Endocrinology. 148 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Acosta TJ: "Vasoactive peptides in bovine ovulatory follicles"Animal Frontier Sciences. 103-108

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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