2002 Fiscal Year Final Research Report Summary
Differential diagnosis and vaccine development of tick-borne encephalitis
| Project/Area Number |
12556051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TAKASHIMA Ikuo Hokkaido Univ., Grad. School of Vet. Med., Prof, 大学院・獣医学研究科, 教授 (30002083)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kenichi Hokkaido Inst. Of Pub. Health., Sect. of Med Zool., Head, 疫学部, 科長
MIZUTANI Tetsuya Hokkaido Univ., Grad. School of Vet. Med., Instr, 大学院・獣医学研究科, 助手 (70281681)
KARIWA Hiroaki Hokkaido Univ., Grad. School of Vet. Med., Assoc. Prof, 大学院・獣医学研究科, 助教授 (70224714)
KURATA Takeshi National Inst of Infectious Diseases, Deputy Director-General, 副所長 (50012779)
NEJIME Tetsuya Serotec Research Institute Deputy Director, 所長代理
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| Project Period (FY) |
2000 – 2002
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| Keywords | Viral encephalitis / Tick-borne encephalitis / Differential diagnosis / Vaccine |
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| Research Abstract |
We conducted epidemiological study to specify the endemic area of tick-borne encephalitis (TBE), evaluated the pathogenicity of TBE virus Hokkaido strain and tested the efficacy of European TBE vaccine to Hokkaido TBE virus strain. We also performed phylogenetic study of TBE virus Hokkaido strainsto estimate the origin.
The results showed that TBE virus distribute in southern part of Hokkaido from the positive antibody results in dog and horse sera. Reservoir animals of TBE virus in Hokkaido were found to be Clethrionomys rufocanus and Apodemus speciosus from virus isolation results. TBE virus Hokkaido strains possessed common pathogenicity shared among TBE virus strains. TBE virus Hokkaido strain diverged from ancestor virus in Far East several hundred years ago. Another subtype of TBE virus, Siberian subtype was found to distribute in Siberian area of Russia. European type TBE virus vaccine was found to be efficacious to TBE virus Hokkaido, Far Eastern and Siberian strains. Monochlonal antibodies against TBE virus Hokkaido strain were produced and found to be useful for diagnosis.
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[Publications] Hayasaka, D., Ivanov, L., Leonova, G. N., Goto, A., Yoshii, K., Mizutani, T., Kariwa, H., Takashima, I.: "Distribution and characterization of tick-borne encephalitis viruses from Siberia and Far-eastern Asia"J. Gen. Virol.. 82. 1319-1328 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Hayasaka, D., Goto, A,. Yoshii, K., Mizutani, T., Kariwa, H., Takashima, I.: "Evaluation of European tick-borne encephalitis virus vaccine against recent Siberian and Far-eastern subtype strains"Vaccine. 19. 4774-4779 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Goto, A., Hayasaka, D., Yoshii, K., Mizutani, T,. Kariwa, H., Takashima, I.: "Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia"Jpn. J. Vet. Res.. 49. 297-307 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Goto, A., Hayasaka, D., Yoshii, K., Mizutani, T., Kariwa, H., Takashima, I.: "A BHK-21 cell culture-adapted tick-borne encephalitis virus mutant is attenuated for neuroinvasiveness"Vaccine. (in press). (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshii, K,. Hayasaka, D., Goto, A., Obara, M., Araki, K., Yoshimatsu, K., Arikawa, J., Ivanov, L. I., Mizutani, T., Kariwa, H., Takashima, I.: "Enzyme-linked immunosorbent assay using recombinant antigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis"J. Virol. Methods. 108(2). 171-179 (2003)
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「研究成果報告書概要(欧文)」より
