2001 Fiscal Year Final Research Report Summary
Development of high sensitive electrochemical DNA microarray
| Project/Area Number |
12557012
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General medical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
UCHIDA Kazuhiro University of Tsukuba, Inst. Basic Med. Sci., Associate Prof., 基礎医学系, 助教授 (90211078)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENAKA Shigeori University of Tsukuba, Inst. Technology, Associate Prof., 大学院・工学研究院, 助教授 (60188208)
ICHIKAWA Yoshihito University of Tsukuba, Inst. Clinical Medicine, Assistanr Prof., 臨床医学系, 講師 (30302425)
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| Project Period (FY) |
2000 – 2001
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| Keywords | human genome / cDNA library / DNA chin / Micriarray |
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| Research Abstract |
It is very important to develop a new DNA chip, which overcomes the problem in the present DNA microarray. In this project, we aim at developing a microelectrode, which immobilizes a synthetic oligonucleotide concerned with vanous genetic diseases and establishing a simple and quick electrochemical detection system for the hybrid with genomic DNA taken from patients. Once realized, this system will allow us to diagnose cancers and infectious diseases efficiently. We have investigated the followings: 1) Study of the preparation technology of microelectrode arrays, 2) Study of the probes of oligonucleotide on the electrode, 3) Study of a technology to analyze electrocheinical data, 4) Establishment of cDNA database for electrochemical array, 5) Study of preparation method of blood samples. We also analyzed genomic alterations in the bile duct cancers and thyroid cancers to identify the valuable genes for genomic diagnosis using electrochernical array. The knowledge thus obtained is useful to develop a potable gene diagnosis instrument to analyze genetic information.
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[Publications] Uchida, M., Hanai, S., Uematsu, N., Sawamoto, K., Okano, H., Miwa, M., Uchida, K.: "Genetic and functional analysis of PARP, a DNA strand break-binding enzyme."Mutat.Res.. 447. 89-96 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uchida, M., Hanai, S., Uematsu, N., Sawamoto, K., Okano, H., Miwa, M., Uchida, K.: "Genetic and functional analysis of PARP, a DNA strand break-binding enzyme."Mutat.Res.. (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, M., Sun, S., Fang, J., Nitta, T., Yoshida, T., Kohtoh, S., Kikukawa, H., Hanai, S., K, Miwa, M.: "HTLV-1 infection to mice: proliferation of cell clones with integrated HTLV-1 provirus in lymphoid organs."J.Virol.. 75(9). 4420-4423 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Kudoh, K., Takano, M., Koshikawa, T., Yoshida, S., Hirai, M., Kikuchi, Y., Nagata, I., Miwa, M., Uchida, K.: "Comparative genomic hybridization for analysis of chromosomal changes in cisplatin-resistant ovarian cancer."Human Cell. 13. 109-116 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Ghosh, M., Kawamoto, T., Koike, N., Fukao, K., Yoshida, S., Kashiwagi, H., Kapoor, V.K., Agarwal, S., Krishnani, N., K, Miwa, M., Todoroki, T.: "Cyclooxygenase expression in the gallbladder."Int.J.Mol.Med.. 6. 527-532 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Miwa, M., Kanai, M., Sun, B., Hirai, M., Xm, X., Sitoh, S., Hanai, S., Kaneko, M., Nakagawara, A., Uchida, K.: "DNA methylation in neuroblastoma, DNA Methylation and Cancer, (S.Hirohashi, et al., ed.)"Princess Takamatsu Cancer Research Fund, Tokyo. (2001)
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「研究成果報告書概要(欧文)」より
