2000 Fiscal Year Annual Research Report

エキノコックス症免疫診断法(国際標準診断、早期診断、治癒判定法)の実用化

Research Project

Project/Area Number	12557024
Research Institution	Asahikawa Medical College
Principal Investigator	伊藤亮旭川医科大学, 医学部, 教授 (70054020)
Co-Investigator(Kenkyū-buntansha)	中尾稔旭川医科大学, 医学部, 助手 (70155670) 藤本佳範旭川医科大学, 医学部, 助手 (90292127) 高後裕旭川医科大学, 医学部, 教授 (10133183) 中谷和宏旭川医科大学, 医学部, 教務職員 (70109388) 迫康仁旭川医科大学, 医学部, 助手 (40312459)
Keywords	新興・再興感染症 / エキノコックス症 / 多包虫症 / 単包虫症 / 有鉤嚢虫症 / 術前鑑別血清診断法 / 早期診断法 / 治癒判定法
Research Abstract	本研究成果の概要は下記の4項目としてまとめられる。エキノコックス症(多包虫症、単包虫症)は平成11年4月1日から施行されている感染症予防新法によって届け出が義務づけられた第4類感染症に含まれている。この法律の施行下で本州から2症例の多包虫症が届けられているが、第1症例は肝蛭症(秋田)第2症例は単包虫症であり、国内で流行が問題になっている多包虫症ではなかった。このような誤診を防ぐ意味からも旭川医大の研究は緊急を要するテーマである。血清学的に鑑別が必要な寄生虫疾患には、多包虫症、単包虫症、有鉤嚢虫症とがあるが、これら3種類の寄生虫疾患についての血清学的鑑別診断法は旭川医大ですでに確立され、国際的にも最も信頼性が高い検査法であると評価されている。本研究では特に多包虫症についての国際標準診断法、早期診断法、治癒判定法の実用化を目的とする研究を推進している。1.Em18抗原を用いる血清診断、血清疫学への展開:中国、四川省、青海省、甘粛省を中心に、Em18-immunoblot陽性者は多包虫症であり、鑑別確定診断法として重要であると評価された。2.早期診断にEm18immunoblotが利用可能かどうかについては、これまでの国内での検査において陰性であった多包虫症例も検出できていることから、早期診断にも利用可能であると予測している。3.イムノブロット用抗原として粗Em18抗原、精製Em18抗原の2種類を用いている。さらに、特異抗体を用いたAffinity精製Em18抗原を用いている。特にAffinity精製Em18抗原を用いている場合に、感度がこれまでよりもかなり高くなることが判明している。4.治癒判定法としては精製Em18抗原、遺伝子組換え技法によって作製しているprototype recEm18(rEMAG5)を用い、ほぼ類似の経過観察により予後判定が可能であるという成績が得られている。

Research Products

(26 results)

All Other

All Publications (26 results)

[Publications] Ito A et al.: "Introduction to the Symposium on Cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Margono SS et al.: "Cysticercosis in Indonesia : epidemiological aspects"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Ito A et al.: "NOD-scid mouse as an experimental animal model for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Okamoto M et al.: "Molecular variation of Taenia solium in the world"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Sako Y et al.: "Recent advances in serodiagnosis for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Ito A et al.: "Differential serodiagnosis of cystic and alveolar echinococcosis using native and recombinant antigens in Japan"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Ito A et al.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 32(in press).
[Publications] Margono SS et al.: "The problem of taeniasis and cysticercosis in Irian Jaya (Papua), Indonesia"Proceedings of the 6th Asian-Pacific Congress for Parasitic Zoonoses. 55. 64 (2000)
[Publications] Ito A et al.: "Comparison of antigenicity of protoscolex and micro vesicle of Echinococcus multilocularis prepared from rats"Journal of Helminthology. (in press)..
[Publications] Wandra T et al.: "Seroepidemiology of neurocysticercosis in Irian Jaya (West Papua), Indonesia in 1996-1997 and Papua New Guinea in 1997"Acta Tropica. 78(in press).
[Publications] Subahar R et al.: "Taenia solium infection in Irian Jaya (West Papua), Indonesia : A pilot serological survey of human and porcine cysticercosis in Jayawijaya District"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press)..
[Publications] Nakao M et al.: "The complete nucleotide sequence of the cestode Echinococcus multilocularis mitochondrial genome"Genetics. (in press)..
[Publications] Jiang L et al.: "Immunodiagnostic differentiation for alveolar and cystic echinococcosis using ELISA test with 18 kDa antigen extracted from Echinocoecus protoscoleces"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press).
[Publications] 伊藤亮: "エキノコックス症-世界・日本での現状、診断・治療・予防-"北海道プライマリー・ケア研究会会報. 19. 29-39. (2001)
[Publications] 伊藤亮: "旭川医科大学におけるエキノコックス症研究の現状と日本におけるエキノコックス症の問題点."北海道医学雑誌. 76,. 3-8. (2001)
[Publications] Craig PS et al.: "An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China"Acta Tropica. 77. 167-177 (2000)
[Publications] Nakao M et al.: "Mitochondrial genetic code in cestodes"Molecular and Biochemical Parasitology. 111. 415-424 (2000)
[Publications] Sako Y et al.: "Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes"Journal of Clinical Microbiology. 38. 4439-4444 (2000)
[Publications] Wang H et al.: "An epidemiological survey on human hydatidosis in southern Qinghai platau"Chinese Journal of Parasitic Disease Control. 13. 37-41 (2000)
[Publications] Wang H et al.: "Infections of larval and adult Echinococcus multilocularis in human and animals in Qinghai province"Chinese Journal of Parasitic Disease Control. 13. 120-123 (2000)
[Publications] Qiu JM et al.: "Epidemiological study on human hydatidosis in Tibetan region of western Sichuan"Chinese Journal of Zoonoses. 16. 77-80 (2000)
[Publications] Wandra,T et al.: "Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia,1991-95"Transactions of the Royal Society of Tropical Medicine and Hygiene. 94. 46-50 (2000)
[Publications] Ito A et al.: "Mitochondrial DNA of Taenia solium : from basic to applied science"Taenia solium Cysticercosis CAB International (UK) Prabhakar S,Singh G (in press).
[Publications] Ito A et al.: "Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS,Pawlowski ZS (in press).
[Publications] Ito A et al.: "Neurocysticercosis in Asia : serology/seroepidemiology in humans and pigs"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS,Pawlowski ZS (in press).
[Publications] 伊藤亮: "第9編原虫学.シンプル微生物学(改訂第3版)"南江堂. (2000)

2000 Fiscal Year Annual Research Report

エキノコックス症免疫診断法(国際標準診断、早期診断、治癒判定法)の実用化

Principal Investigator

伊藤 亮 旭川医科大学, 医学部, 教授 (70054020)

Research Products

[Publications] Ito A et al.: "Introduction to the Symposium on Cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Margono SS et al.: "Cysticercosis in Indonesia : epidemiological aspects"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Ito A et al.: "NOD-scid mouse as an experimental animal model for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Okamoto M et al.: "Molecular variation of Taenia solium in the world"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Sako Y et al.: "Recent advances in serodiagnosis for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Ito A et al.: "Differential serodiagnosis of cystic and alveolar echinococcosis using native and recombinant antigens in Japan"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Ito A et al.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 32(in press).

[Publications] Margono SS et al.: "The problem of taeniasis and cysticercosis in Irian Jaya (Papua), Indonesia"Proceedings of the 6th Asian-Pacific Congress for Parasitic Zoonoses. 55. 64 (2000)

[Publications] Ito A et al.: "Comparison of antigenicity of protoscolex and micro vesicle of Echinococcus multilocularis prepared from rats"Journal of Helminthology. (in press)..

[Publications] Wandra T et al.: "Seroepidemiology of neurocysticercosis in Irian Jaya (West Papua), Indonesia in 1996-1997 and Papua New Guinea in 1997"Acta Tropica. 78(in press).

[Publications] Subahar R et al.: "Taenia solium infection in Irian Jaya (West Papua), Indonesia : A pilot serological survey of human and porcine cysticercosis in Jayawijaya District"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press)..

[Publications] Nakao M et al.: "The complete nucleotide sequence of the cestode Echinococcus multilocularis mitochondrial genome"Genetics. (in press)..

[Publications] Jiang L et al.: "Immunodiagnostic differentiation for alveolar and cystic echinococcosis using ELISA test with 18 kDa antigen extracted from Echinocoecus protoscoleces"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press).

[Publications] 伊藤亮: "エキノコックス症-世界・日本での現状、診断・治療・予防-"北海道プライマリー・ケア研究会会報. 19. 29-39. (2001)

[Publications] 伊藤亮: "旭川医科大学におけるエキノコックス症研究の現状と日本におけるエキノコックス症の問題点."北海道医学雑誌. 76,. 3-8. (2001)

[Publications] Craig PS et al.: "An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China"Acta Tropica. 77. 167-177 (2000)

[Publications] Nakao M et al.: "Mitochondrial genetic code in cestodes"Molecular and Biochemical Parasitology. 111. 415-424 (2000)

[Publications] Sako Y et al.: "Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes"Journal of Clinical Microbiology. 38. 4439-4444 (2000)

[Publications] Wang H et al.: "An epidemiological survey on human hydatidosis in southern Qinghai platau"Chinese Journal of Parasitic Disease Control. 13. 37-41 (2000)

[Publications] Wang H et al.: "Infections of larval and adult Echinococcus multilocularis in human and animals in Qinghai province"Chinese Journal of Parasitic Disease Control. 13. 120-123 (2000)

[Publications] Qiu JM et al.: "Epidemiological study on human hydatidosis in Tibetan region of western Sichuan"Chinese Journal of Zoonoses. 16. 77-80 (2000)

[Publications] Wandra,T et al.: "Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia,1991-95"Transactions of the Royal Society of Tropical Medicine and Hygiene. 94. 46-50 (2000)

[Publications] Ito A et al.: "Mitochondrial DNA of Taenia solium : from basic to applied science"Taenia solium Cysticercosis CAB International (UK) Prabhakar S,Singh G (in press).

[Publications] Ito A et al.: "Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS,Pawlowski ZS (in press).

[Publications] Ito A et al.: "Neurocysticercosis in Asia : serology/seroepidemiology in humans and pigs"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS,Pawlowski ZS (in press).

[Publications] 伊藤亮: "第9編 原虫学.シンプル微生物学(改訂第3版)"南江堂. (2000)

伊藤亮旭川医科大学, 医学部, 教授 (70054020)

[Publications] 伊藤亮: "第9編原虫学.シンプル微生物学(改訂第3版)"南江堂. (2000)