| Co-Investigator(Kenkyū-buntansha) |
IWAKURA Yoichiro The Institute of Medical Science, The University of Tokyo, Professor, 医科学研究所, 教授 (10089120)
NOROSE Kazumi Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (30156244)
AOSAI Fumie Chiba University, Graduate School of Medicine, Assistant Professor, 大学院・医学研究院, 助教授 (80150316)
SEKI Naohiko Chiba University, Graduate School of Medicine, Assistant Professor, 大学院・医学研究院, 助教授
TSUJIMOTO Atsumi DNA Chip Institute, Senior investigator, 主任研究員
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| Research Abstract |
By using our own made cDNA microarray system composed with around 15,000 clones established by using mRNA from normal mice, Toxoplasma gondii-infection-induced genes were analyzed Five gene groups, beta2-microblobulin, DMR, spot 14, teststeron, and an unknown (tentatively named as T.g.Res.X ) were picked up. T.g.Res.X was shown to express in a resistant strain to T.gondii infection when the mRNA expression was examined by comparing between a wild type resistant strain(BALB/c) and a wild type susceptible strain( C57BL/6), and also comparing between wild type and IFN-_γ KO BALB/c mice.
Effects of interferon-gamma (IFN-_γ) on stage conversion between bradyzoites and tachyzoites of Toxoplasma gondii were investigated by measuring a virulent gene of T.gondii, heat shock protein 70 (T.g.HSP70), a bradyzoite-specifc gene, T.g.HSP30/bag1, and a tachyzoite-specific gene, SAG1 mRNAs with cDNA microarray method in wild type and IFN-γ knockout (KO) mice. In wild type mice, T.g.HSP30/bag1 mRNA expression continued for 3 days post infection(PI), while SAG1 mRNA expressed for 9 days PI. In IKO mice, T.g.HSP30/bag1 mRNA expression decreased drastically 24 hr post PI, and SAG1 mRNA began to express interchangeably. Then T.g.HSP70 mRNA expressed rapidly 7-8 days PI, and the mice died just after T.g.HSP70 mRNA expression. Thus, IFN-γ downregulates SAG1 and T.g.HSP70 mRNA expression.
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