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2001 Fiscal Year Final Research Report Summary

Antinucleic aid antibody gene therapy for rheumatoid Arthritis and adult T cell Leukemia

Research Project

Project/Area Number 12557044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 内科学一般
Research InstitutionTohoku University

Principal Investigator

SASAKI Takeshi  Tohoku Univ., Medicine, Professor, 大学院・医学系研究科, 教授 (50110656)

Co-Investigator(Kenkyū-buntansha) KAMEOKA Junichi  Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (30261621)
HARIGAE Hideo  Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (50302146)
KITAGAWA Yoshichika  Akita Univ., Ins Biotech., Professor, 生物工学研究所, 教授 (10006752)
MUNAKATA Yasuhiko  Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (20271950)
Project Period (FY) 2000 – 2001
KeywordsGene therapy / antinucleic acid antibody / retrovirus / adult T cell leukemia virus
Research Abstract

1.Expression of antibody against DNARNA hybrid in the cells.
We first obtained hybridoma producing monoclonal anti-DMA RNA hybrid antibody and prepared the fragments of VH and VL gene encoding the antibody. Both fragments were bound for the expression of ScFv antibodies and introduced into vectors for the expression in the cells.
2. Efficacy of gene therapy using anti-ScFv expression system for prevention of tabacco mosaic virus (TMV) infection.
Tabacco which was introduced with anti-DNARNA hybrid ScFv genes, expressed ScFv mRNA and showed marked resistance to TMV infection.
3. Blocking experiment of human adult T cell leukemia virus (HTLV-1) infection by gene therapy using anti-ScFv expression system.
We are establishing for evaluation of efficacy of ScFv strategy in order to block HTLV-1 infection in T cells.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Suzuki, T.Funato, T.Sasaki: "Chemically modified Ribozyme Targeting TNF-αMRNA regulates TNF-α and IL-6 Synthesis in Synovial Fibroblasts of Patients with Rheumatoid Arthritis"J. Clin. Immunol. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Fu, T.Sasaki et al.: "Regulation of TNF-α promoter by human parvovirus B19 NS1 through activation Of AP-1 and AP-2"J. Virology.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Sasaki, T.Sasaki, ほか: "Structural alteration of retinoblastoma protein-interacting zinc finger gene, RIZ, in human keukaemia"Tohoku. J. Exp. Med.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Harigae, T.Sasaki ほか: "Primary marginal zone lymphoma of the thymus accompanied by chromosomal anomaly, 46X, dup(x)(p11p22)"Cancer Genetics cytogenetics.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Kameoka, T.Sasaki ほか: "Autoimmune neutropenia in pregnant women causing neonatural neutropenia"Br J Haematol.. 114. 198-200 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Takahashi, T.Sasaki ほか: "Genetic structure and regulation of a novel human gene, Klo1"Biochem, Bioph, Acta.. 91600. 1-5 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Kameoka, T. Funato. T.Miura, H.Harigae, J.Saito, H.Yokoyama, S.Takahashi, M.Yamada, O.Sasaki, M.Imaizumi, N.Takata, K.Meguro, T.Sasaki: "Autoimmune neutropenia in pregnant women causing neonatural neutropenia"Br J Haematol.. 114. 198-200 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Takahashi, H.Harigae, H.Yokoyama M.Kaku, T.Sasaki: "Genetic structure and regulation of a novel human gene, Klp1^1"Biochem. Bioph. Acta.. 91600. 1-5 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Yi, K.K.Ishii, Y.Munakata, T.Saitoh, M.Kaku, T.Sasaki: "Regulation of TNF-α promoter by human parvovirus B19 NS1 through activation of AP-1 and AP-2"J.Virology. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Takahashi, T.Funato, Y.Suzuki, H.Fujii, K.Kumuraishi, M.Kaku, T.Sasaki: "Chemically modified Ribozyme Targeting TNF-α mRNA rugulates TNF-α and IL-6 Synthesis in Synovial Fibroblasts of Patients with Rheumatoid Arthritis"J. Clin. Immunol.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Harigae, R.Ichinohasama, I.Miura, J.Kameoka, K.Meguro, K.Miyamura, O.Sasaki, I.Ishikawa, S.Takahashi, M.Kaku, T.Sasaki: "Primary marginal zone lymphoma of the thymus accompanied by chromosomal anomaly, 46X,dup(X)(P11 P22)"Cancer Genetics cytogenetics.. (In press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O.Sasaki, K.Meguro, Y.Tohmiya, T.Funato, S.Shibahara, T.Sasaki: "Structural alteration of retionblastoma protein-interacting zinc finger gene, RIZ, in human leukemia"Tohoku.J.Exp.Med.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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