2001 Fiscal Year Final Research Report Summary
Antinucleic aid antibody gene therapy for rheumatoid Arthritis and adult T cell Leukemia
| Project/Area Number |
12557044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
内科学一般
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| Research Institution | Tohoku University |
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Principal Investigator |
SASAKI Takeshi Tohoku Univ., Medicine, Professor, 大学院・医学系研究科, 教授 (50110656)
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| Co-Investigator(Kenkyū-buntansha) |
KAMEOKA Junichi Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (30261621)
HARIGAE Hideo Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (50302146)
KITAGAWA Yoshichika Akita Univ., Ins Biotech., Professor, 生物工学研究所, 教授 (10006752)
MUNAKATA Yasuhiko Tohoku Univ. Hospital, Research Associate, 医学部・付属病院, 助手 (20271950)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Gene therapy / antinucleic acid antibody / retrovirus / adult T cell leukemia virus |
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| Research Abstract |
1.Expression of antibody against DNARNA hybrid in the cells.
We first obtained hybridoma producing monoclonal anti-DMA RNA hybrid antibody and prepared the fragments of VH and VL gene encoding the antibody. Both fragments were bound for the expression of ScFv antibodies and introduced into vectors for the expression in the cells.
2. Efficacy of gene therapy using anti-ScFv expression system for prevention of tabacco mosaic virus (TMV) infection.
Tabacco which was introduced with anti-DNARNA hybrid ScFv genes, expressed ScFv mRNA and showed marked resistance to TMV infection.
3. Blocking experiment of human adult T cell leukemia virus (HTLV-1) infection by gene therapy using anti-ScFv expression system.
We are establishing for evaluation of efficacy of ScFv strategy in order to block HTLV-1 infection in T cells.
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[Publications] J.Kameoka, T. Funato. T.Miura, H.Harigae, J.Saito, H.Yokoyama, S.Takahashi, M.Yamada, O.Sasaki, M.Imaizumi, N.Takata, K.Meguro, T.Sasaki: "Autoimmune neutropenia in pregnant women causing neonatural neutropenia"Br J Haematol.. 114. 198-200 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] H.Harigae, R.Ichinohasama, I.Miura, J.Kameoka, K.Meguro, K.Miyamura, O.Sasaki, I.Ishikawa, S.Takahashi, M.Kaku, T.Sasaki: "Primary marginal zone lymphoma of the thymus accompanied by chromosomal anomaly, 46X,dup(X)(P11 P22)"Cancer Genetics cytogenetics.. (In press). (2002)
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「研究成果報告書概要(欧文)」より
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