2001 Fiscal Year Final Research Report Summary
Induction of differentiation of monkey embryonic stem cells into tissue-specific functional cells
| Project/Area Number |
12557067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Pediatrics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TSUJI Kohichiro The Institute of Medical Science, The University of Tokyo, Associate Professor, 医科学研究所, 助教授 (50179991)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Ryuhei Biology Division, National Cancer Center, Research Institute, Section Head (Researcher), 生物学部, 室長(研究職) (80322072)
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| Project Period (FY) |
2000 – 2001
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| Keywords | monkey embryonic stem cells / AGM region / stromal cells / NOD / SCID mice |
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| Research Abstract |
1. Characterization of rhesus embryonic stem (rES) cells
rES cells were maintained by the coculture with mouse embryonic fibroblasts. The maintained rES cells expressed alkaliphaphatase, SSEA1 and SSEA4. When the rES cells were transplanted into NOD/SCID mice through the tail vain, they produced teratoma in the recipient's lung. These results indicate the immature nature of rES cells.
2. Differentiation of rES cells
When the rES cells were cocultured with stromal cells derived from aorta-gonad-mesonephros (AGM) region in 10.5 days postcoitum (dpc) mouse embryo, they formed cobble stone areas, which generated small round cells after 2 weeks of coculture. Flowcytometric analysis showed that a part of the generated cells expressed human CD34. We are further characterizing the cells expressing human CD34.
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[Publications] Yoshimasu, T, Tanaka R, Suenobu S, Yagasaki H, Yoshino H, Hisakawa H, Ishii T, Mitsui T, Ebihara Y, Manabe A, Nakahata T, Maekawa T, Asano S, Tsuji K: "Prompt and durable hematopoietic reconstitution by an unrelated cord blood transplantation in a child with Fanconi anemia"Bone Marrow Transplant. 27. 767-769 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ma F, Wada M, Ebihara Y, Ishii F, Manabe A, Tanaka R, Maekawa T, Ito M, Mugishima H, Asano S, Nakahata T, Tsuji K: "Development of human lymphohematopoietic stem/progenitor cells defined by CD34 and CD81 expressions"Blood. 97. 3755-3762 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ueda T, Yoshida M, Yoshino H, Kobayashi K, Kawahata, M, Ebihara Y, Ito M, Asano S, Nakahata T, Tsuji K: "Hematopoietic capability of cord blood CD34^+ cells : a comparison with that of adult bone marrow CD34^+ cells"Int J Hematol. 73. 457-462 (2001)
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[Publications] Matsuoka S, Tsuji K. Hisakawa H, Xu M, Ebihara Y, Ishii T, Sugiyama D, Manabe A, Tanaka R, Ikeda Y, Asano S, Nakahata T: "Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleura by aorta-gonad-mesonephros region-derived stromal cells."Blood. 98. 6-12 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Hisakawa H, Sugiyama D, Nishijima I, Hong Wu, Nakao K, Watanabe S, Katsuki M, Asano S, Arai K, Nakahata T, Tsuji K: "Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) stimulates primitive and definitive erythropoiesis in mouse embryo expressing hGM-CSF receptors but not erythropoietin receptors"Blood. 98. 3618-3625 (2001)
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