2003 Fiscal Year Final Research Report Summary
HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE -STIMULATING HORMONE SUBUNITE GENE
| Project/Area Number |
12557087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Endocrinology
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| Research Institution | SCHOOL OF AGRICULTURE |
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Principal Investigator |
KATO Yukio MEIJI UNIVERSITY, SCHOOL OF AGRICULTURE, PROFESSOR, 農学部, 教授 (30114177)
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| Project Period (FY) |
2000 – 2003
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| Keywords | PITUITARY / RECOMBINANT DNA / GONADOTROPIN / CLONING / TRANSCRIPTION FACTOR / GENE / HOMEOBOX / YEST ONE HYBRID |
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| Research Abstract |
(1)FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.
(2)THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.
(3)PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSHβ GENE
(4)EXPRESSION. VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHI3 GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.
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[Publications] Moriyama, R., Tsukamura, H., Kinoshita, M., Okazaki.H., Kato, Y., Maeda, K.-I.: "In vitro Increase In Intracellular Calcium Concentrations Induced by Low or High Extracellular Glucose Levels in Ependymocytes and Serotonergic Neurons of the Rat Lower Brain"Endocrinology. (印刷中).
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[Publications] Chen L, Maruyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y, Inoue K: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neural nitric oxide synthetase expression and nitric oxide secretrion."Endocrinology. 141. 3603-3310 (2000)
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[Publications] Tomizawa K, Ogawa S, Kato T, Watanabe M, Sato I, Sakai T, Hattor M-A, Inoue K, Ueda S, Kikuchi M, Mori J, Kato Y: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev. 48. 131-136 (2002)
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