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2002 Fiscal Year Final Research Report Summary

New strategy for cancer therapy using Hi-resolution microsatellite instability analysis and Real-time quantitative reverse transcriptase polymerase reaction

Research Project

Project/Area Number 12557101
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MAEHARA Yoshihiko  Kyushu University, Surgery and Science, professor, 大学院・医学研究院, 教授 (80165662)

Co-Investigator(Kenkyū-buntansha) TOKUNAGA Eriko  Kyushu University, Hospital, Instructor, 医学部附属病院, 助手 (50325453)
KAKEJI Yoshihiro  Kyushu University, Hospital, Assistant professor, 医学部附属病院, 講師 (80284488)
Project Period (FY) 2000 – 2002
KeywordsReal-time PCR / micrometastasis / peripheral blood / bone marrow / microsatellite instability / mismatch repair / genetic analysis
Research Abstract

Micrometastasis; Real time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect disseminated cancer cells in bone marrow and peripheral blood. We compared CEA, CK18, CK19 and CK20 expression using 20 of cell lines in order to select the most appropriate. In an analysis of CK 18 mRNA expression in peripheral venous blood in 13 healthy volunteers, we found that CK 18 mRNA was much less expressed than in cancer cell lines. However, the expression in all samples was at a level which was also detected using conventional RT-PCR. It would thus seem that not only qualitative, but also quantitative analysis, of the target mRNA is important to detect micrometastasis. Quantitative RT-PCR methods will make comparisons of the possible differences in expression levels of the target gene. For clinical applications, much further study is needed.
Microsalellite Instability; Using high-resolution fluorescent microsatellite analysis (HFRMA), we studied over 800 patients with sporadic breast, gastric, colon, lung and liver cancer. In this system, several devices were prepared to improve reproducibility of polymerase chain reaction products, migration accuracy of electrophoresis, and characteristics of the detection system. All the microsatellite changes observed in this system can be classified into two types: type A with relatively small changes in microsatellite sequences observed in limited loci and type B with drastic and widely dispersed changes. The former was thought to be connected to abnormal activity in DNA mismatch repair (MMR).

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yoshino I, et al.: "Detection of loss of heterozygosity by high-resolution fluorescent system in non-small cell lung cancer : association of loss of heterozygosity with smoking and tumor progression"Chest. 123(2). 545-550 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maehara Y, et al.: "Diagnosis and treatment for familial gastric cancer"癌と化学療法. 29(4). 523-531 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oda S, et al.: "Microsatellite instability in cancer : what problems remain unanswered?"Surgery. 131(1Suppl). S55-S62 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda Y, et al.: "Features of microsatellite instability in colorectal cancer : comparison between colon and rectum"Oncology. 61(2). 168-174 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maehara Y, et al.: "Tumor growth patterns and biological characteristics of early gastric carcinoma"Oncology. 61(2). 102-112 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maehara Y, et al.: "The instability within : problems in current analyses of microsatellite instability"Mutat Res.. 461(4). 249-263 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maehara Y, et al.: "Time trends of surgical treatment and the prognosis for Japanese patients with gastric cancer"Br J Cancer. 83(8). 986-991 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga E, et al.: "Frequency of microsatellite instability in breast cancer determined by high resolution fluorescent microsatellite analysis"Oncology. 59(1). 44-49 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oki E, et al.: "Detection of disseminated cancer cells in bone marrow of gastric cancer using real time quantitative reverse transcriptase polymerase chain reation"Cancer Lett.. 188(1-2). 191-198 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga E, et al.: "Application of quantitative RT-PCR using "TaqMan" technology to evaluate the expression of CK 18 mRNA in various cell lines"J Exp Cli cancer Res.. 19(3). 375-381 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga E, et al.: "Frequency of microsatellite instability in breast cancer determined by high-resolution fluorescent microsatellite analysis"Oncology. 59(1). 44-49 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga E, et al.: "Diminished expression of ING1 mRNA and the correlation with p53 expression in breast cancers"Cancer Lett.. 152(1). 15-22 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshino I, et al.: "Detection of loss of heterozygosity by high-resolution fluorescent system in non-small cell lung cancer: association of loss of heterozygosity with smoking and tumor progression."Chest. 123(2). 545-550 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maehara Y, et al.: "Diagnosis and treatment for familial gastric cancer."29(4). 523-531 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oda S, et al.: "Microsatellite instability in cancer: what problems remain unanswered?"Surgery. 131(1Suppl). S55-S62 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda Y, et al.: "Features of microsatellite instability in colorectal cancer: comparison between colon and rectum."Oncology. 61(2). 168-174 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maehara Y, et al.: "Tumor growth patterns and biological characteristics of early gastric carcinoma."Oncology. 61(2). 102-112 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maehara Y, et al.: "The instability within: problems in current analyses of microsatellite instability."Mutat Res.. 461(4). 249-263 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maehara Y, et al.: "Time trends of surgical treatment and the prognosis for Japanese patients with gastric cancer."Br J Cancer. 83(8). 986-991 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga E, et al.: "Frequency of microsatellite instability in breast cancer determined by high resolution fluorescent microsatellite analysis."Oncology. 59(1). 44-49 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oki E, et al.: "Detection of disseminated cancer cells in bone marrow of gastric cancer using real time quantitative reverse transcriptase polymerase chain reation."Cancer Lett.. 188(1-2). 191-198 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga E, et al.: "Application of quantitative RT-PCR using "TaqMan" technology to evaluate the expression of CK 18 mRNA in various cell lines."J Exp Clin cancer Res.. 19(3). 375-381 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga E, et al.: "Frequency of microsatellite instability in breast cancer determined by high-resolution fluorescent microsatellite analysis."Oncology. 59(1). 44-49 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga E, et al.: "Diminished expression of ING1 mRNA and the correlation with p53 expression in breast cancers."Cancer Lett.. 152(1). 15-22 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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