2001 Fiscal Year Final Research Report Summary
Analysis of novel tumor suppressor gene AML2 in gastric cancer
| Project/Area Number |
12557107
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto prefectural University of Medicine |
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Principal Investigator |
SAKAKURA Chouhei Kyoto Prefectural University of Medicine, Dept. of Digestive Surgery, Stuff, 医学部, 助手 (10285257)
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA Tsukasa Kyoto Prefectural University of Medicine, Dept. of Hygiene, Stuff, 医学部, 講師 (30291587)
ITO Yoshiaki Kyoto University, Virus Institute, Professor, ウイルス研究所, 教授 (80004612)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Gastric cancer / AML2 (RUNX3) |
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| Research Abstract |
The human runt-related gene AML2 (RUNX3), located on chromosome 1p36, is a major mediator of signals elicited by members of the transforming growth factor-β (TGF-β) super family. Here we show that 45 - 60 % of gastric cancer cell lines and surgically resected specimens do not significantly express AML2 (RUNX3) due to a combination of hemizygous deletion and hypermethylation of the AML2 (RUNX3) promoter region. Tumorigenicity of gastric cancer cell lines in nude mice was inversely related to their level of AML2 (RUNX3) expression, and one gastric tumor associated mutation (R122C), occurring within the conserved Runt domain completely abolished the tumor suppressive effect of AML2 (RUNX3). The results suggest that a lack of AML2 (RUNX3) function is causally related to the genesis and progression of human gastric cancer.
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[Publications] Sakakura C., Hagiwara A, Shirasu M., Yasuoka R., Fujita Y., Nakanishi M., Aragane H., Masuda K., Shimomura K., Abe T., Yamagishi H. Related Articles: "Polymerase chain reaction for detection of carcinoembryonic antigen-expressing tumor cells on milky spots of the greater omentum in gastric cancer patients : a pilot study"Int J Cancer. 95 (5). 286-9 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Sakakura C., Hagiwara A., Yasuoka R., Fujita Y., Nakanishi M., Masuda K., Shimomura K., Nakamura Y., Inazawa j., Abe T., Yamagishi H. Related Articles: "Tumour-amplified kinase BTAK is amplified and overexpressed in gastric cancers with possible involvement in aneuploid formation"Br J Cancer. 84 (6). 824-31 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sakakura C., Hagiwara A., Yasuoka R., Fujita Y., Nakanishi M., Masuda K., Kimura A., Nakamura Y., Inazawa J., Abe T., Yamagishi H.: "Amplification and over-expression of the AIB1 nuclear receptor co-activator gene in primary gastric cancers"Int J Cancer. 89 (3). 217-23 (2000)
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「研究成果報告書概要(欧文)」より
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