Therapy of neuropathic pain by using a gene-based approach of NF-κB decoy

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12557130
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 展開研究
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka University
• Principal Investigator: MASHIMO Takashi Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (60157188)
• Co-Investigator(Kenkyū-buntansha): SHIBATA Masahiko Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (50216016) ; KIYONO Hiroshi Osaka University, Research Institute for Microbial Deseases, Professor, 微生物病研究所, 教授 (10271032)
• Project Period (FY): 2000 – 2002
• Keywords: NF-κB / decoy / heuropathic pain / cytokine / HVJ-liposome / 遺伝子導入

Research Abstract

Pro-inflammatory cytokines have been shown to be involved in the genesis, persistence, and severity of neuropathic pain following nerve injury. The transcription factor, nuclear factor-kappa B (NF-κB), palys a pivotal role in regulating pro-inflammatory cytokine gene expression. To elucidate the role of NF-κB in the pathogenesis of neuropathic pain, using a gene-based approach of NF-κB decoy, we tested whether the activated NF-κB affected pain behavior via the expression of inflammatory mediators. Single endoneurial injections' of, NF-κB decoy, at the site of nerve lesion, significantly alleviated thermal hyperalgesia for up to 2 weeks and suppressed the expression of mRNA of the inflammatory cytokines, iNOS, and adhesion molecules at the site of nerve injury. This finding suggests that a perineural inflammatory cascade, that involves NF-κB, is involved in the pathogenesis of neuropathic pain

Research Products

• [Publications] Sakaue G, Shimaoka M, Fukuoka T, Hiroi T, Inoue T, Hashimoto N, Sakaguchi T, Sawa Y, Morishita R, Kiyono H, Noguchi K, Mashimo T: "NF-κB decoy supresses cytokine expression and thermal hyperalgesia in a rat neuropathic pain model."NeuroReport. 12. 2079-2084 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Dai Y, Obata K, Mashimo T, Noguchi K: "Expression of HGF and cMet in the peripheral nervous system of adults rats following sciatic nerve injury."NeuroReport. 12. 1403-1407 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Obata K, Mashimo T, Noguchi K.: "Expression of hepatocyte growth factor in primary sensory neurons of adult rats."Mol Brain Res. 97. 83-88 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakaue G, Shimaoka M, Fukuoka T, Hiroi T, Inoue T, Hashimoto N, Sakaguchi T, Sawa Y, Moricita R, Kiyono H, Noguchi K, Mashimo T.: "NF-κB decoy supresses cytokine expression and thermal hyperalgesia in a rat neuropatic pain model"NeuroReport. 12. 2079-2084 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Dai Y, Obata K, Mashimo T, Noguchi K.: "Expression of HGF and cMet in the peripheral nervous system of adult rats following sciatic nerve injury"NeuroReport. 12. 1403-1407 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hashimoto N, Yamanaka H, Fukuoka T, Obata K, Mashimo T, Noguchi K.: "Expression of hepatocyte growth factor in primary sensory neurons of adult rats"Mol Brain Res. 97. 83-88 (2001)
  • Description: 「研究成果報告書概要(欧文)」より