| Project/Area Number |
12557154
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Functional basic dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
TAKIGAWA Masaharu Okayama University, Guraduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (20112063)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Takashi Okayama University, Guraduate School of Medicine and Dentistry, Instructor, 大学院・医歯学総合研究科, 助手 (30322233)
KUBOTA Satoshi Okayama University, Guraduate School of Medicine and Dentistry, Instructor, 大学院・医歯学総合研究科, 助手 (90221936)
NAKANISHI Tohru Okayama University, Guraduate School of Medicine and Dentistry, Associate Professor, 大学院・医歯学総合研究科, 助教授 (30243463)
MUKUDAI Yoshiki Okayama University, Dental School , Research Technician, 歯学部, 教務員 (50325099)
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| Project Period (FY) |
2000 – 2001
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| Keywords | CTGF / chondrocytes / osteoblasts / CCN family / ELISA / Cell differentiation / angiogenesis(diseases) / hypoxia |
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| Research Abstract |
Expression vectors for IGF binding protein-like(IGFBP), von Willebrand type C repeat(VWC), thrombospondin type I repeat(TSP1) and C-terminal(CT) domains of CTGF/ecogenin were constructed and their recombinant proteins were produced.
Plasmids of CTGF lacking 1 or 2 domains were constructed and introduced into HeLa cells. Consequently, HeLa cell lines producing these proteins were established.
Using the recombinant proteins, epitopes of six monoclonal antibodies and two polyclonal antibodies were determined. Three types of ELISA systems were developed.
After production, CTGF was processed before it was secreted out of cells.
CTGF was found to be a hypoxia-induced angiogenic factor, tumor angiogenesis factor and mechanical stress-induced factor. The ELISA systems described above could be used for diseases related with these phenomina.
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