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2001 Fiscal Year Final Research Report Summary

Grapefruit juice-drug interaction and the prediction

Research Project

Project/Area Number 12557225
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用薬理学・医療系薬学
Research InstitutionTohoku University

Principal Investigator

YAMAZOE Yasushi  Tohoku Univ., Grad. Sch. of Pharm. Sci., Prof., 大学院・薬学研究科, 教授 (00112699)

Co-Investigator(Kenkyū-buntansha) OHASHI Kyoichi  Hamamatsu Med. Univ., Prof., 教授 (20137714)
FUKUDA Katsuyuki  Tanabe Pharm. Co., Res., 研究院
NAGATA Kiyoshi  Tohoku Univ., Grad. Sch. of Pharm. Sci., Ass. Prof., 大学院・薬学研究科, 助教授 (80189133)
Project Period (FY) 2000 – 2001
Keywordsgrapefruit juice / drug interaction / drug metabolism / enzyme inhibition
Research Abstract

Grapefruit-drug interaction was investigated to understand the mechanism and influence on drug therapy. We first identified the causative chemicals in grapefruit juice as furanocoumarin-geranylderivatives including GF-I-1 and GF-I-4. These chemicals were found to be strong inhibitors of a human cytochrome P450, CYP3A4. We showed that less than 50 ml intake of the juice altered the kinetic profile of midazolam, which was metabolized by CYP3A4. Selective influence of the juice on intestinal CYP3A4 was shown with volunteers taking omeprazole. Furanocoumarins were shown to decrease intestinal CYP3A4 by both competitive and inactivation, possibly through intermediate complex formation. In addition, we found that grapefruit intake suppress DNA lesion induced by dietary carcinogen PhIP. We are currently investigating the chemoprevention mechanism. To assess the influence of the juice component on CYP3A4 induction, we are developing the in vitro assay system of CYP3A4 gene activation. We have a plan to test the furanocoumarins for the inducibility of CYP3A4.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] S.Matsumoto: "Involvement of multiple human cytochrome P450 in the liver microsomal metabolism of astemizole and a comparison with terfenadine"Br. J. Clin. Pharmacol.. 51. 133-142 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] L.-Q.Guo: "inhibitory potential of herbal medicines on human cytochrome P450-mediated oxidation : Properties of Umbelliferous or Citrus crude drugs and their relative prescriptions"Jpn. J. Pharmacol.. 85. 399-408 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Furukawa: "Adenovirus vector-mediated reporter system for in vivo analyses of human CYP3A4 gene activation"J. Biochem. 131. 71-78 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ogino: "Selective suppressions of human CYP3A form CYP3A5 and CYP3A7,by troglitazane in HepG2 cells"Drog Metabol. Pharmacokin.. 17. 42-46 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Miyata: "Suppression of 2-amino-1-methyl-6-phenylimedazo[4,5-b]pyridine-induced DNA damage in rat colon after grapefruit juice intake"Cancer Lette.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Matsumoto: "Involvement of multiple human cytochrome P450 in the liver microsomal metabolism of astemizole and a comparison with terfenadine"Br. J. Clin. Pharmacol.. 51. 133-142 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] L.-Q. Guo: "Inhibitory potential of herbal medicines on human cytochrome P450-mediated oxidation : Properties of Umbelliferous or Citrus crude drugs and their relative prescriptions"Br. J. Clin. Pharmacol.. 85. 399-408 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Furukawa: "Adenovirus vector-mediated reporter system for in vivo analyses of human CYP3A4 gene activation"J. Biochem.. 131. 71-78 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Ogino: "Selective suppressions of human CYP3A form, CYP3A5 and CYP3A7, by troglitazane in HepG2 cells"Drug Metabol. Pharmacokin.. 17. 42-46 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Miyata: "Suppression of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induce d DNA damage in rat colon after grapefruit juice intake"Cancer Lette.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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