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2002 Fiscal Year Final Research Report Summary

Development of new SNP typing technologies for searching disease-associated genes.

Research Project

Project/Area Number 12557237
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory medicine
Research InstitutionThe University of Tokyo

Principal Investigator

TOKUNAGA Katsushi  The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (40163977)

Co-Investigator(Kenkyū-buntansha) YAMANE Akio  Wakunaga Pharmaceutical Co., Ltd. Institution for Medical Research, Associate Director (Researcher), 創薬研究所, 副所長(研究職)
KARAKI Sachiko  Olympus Optical Co., Ltd. Biomedical Research Center, Manager & Senior Researcher (Researcher), 基礎技術研究所, 主任研究員(研究職)
TSUCHIYA Naoyuki  The University of Tokyo, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60231437)
Project Period (FY) 2000 – 2002
Keywordssingle nucleotide polymorphism (SNP) / SNP typing technology / DNA micro array / disease associated gene / rheumatoid arthritis / systemic lupus erythematosus
Research Abstract

In order to establish reliable technologies for typing nucleotide sequence polymorphisms, we first developed a new typing system for the HLA-DRB1 gene, which shows a highest degree of polymorphism and significant associations with many diseases. A glass array with a chamber made the typing procedure similar to that for microtiter plates. Next we developed a micro-capillary array for the typing of disease-associated SNPs. Many different oligonucleotide probes were spotted in each capillary, and each probe provided a specific hybridization signal after washing through a capillary. Thus this system may be suitable for multiplex and automated SNP typing. In addition, we could establish a new SNP typing system in the combination of PCR-SSP with the fluorescence correlation spectroscopy. The system has been introduced to routine SNP typing and proved to be a large-scale and cost-effective system. Furthermore, we have identified several rheumatic disease-associated SNPs including those of FCGR2B, FCGR3A and LIR1 genes.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kawasaki A, et al.: "Independent contribution of HLA-DRB1 and TNFa promoter polymorphisms to the susceptibility to Crohn^ユs disease"Genes Immun.. 1. 351-357 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuchiya N, et al.: "Analysis of the asscciation of HLA-DRB1, TNFa promoter and TNFR2(TNFRSF1B)polymorphisms with SLE using transmission disequilibrium test"Genes Immun.. 2(6). 317-322 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato M, et al.: "Identification of novel single nucleotide substitutions in the NKp30 gene expressed in human natural killer cells"Tissue Antigens. 58(4). 255-258 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kyogoku C, et al.: "Fcg receptor gene polymorphisms in Japanese patients with systemic lupus erythematosus : Contribution of FEGR2B to genetic susceptibility"Arthritis Rheum.. 46(5). 1242-1254 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroki K, et al.: "Polymorphisms of human CD19 gene : Possible association with susceptibility to systemic lupus erythematosus in Japanese"Genes. Immun.. 3. 21-30 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawasaki A, et al.: "Analysis on the association of human BLYS (BAFF, TNFSF13B)polymorphisms with systemic lupus erythematosus and rheumatoid arthritis"Genes. Immun.. 3(7). 424-429 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuchiya N, et al.: "Immunological Reviews 2002, Vol.190"Blackwell Munksgaard. 1-206 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato H, et al.: "Single nucleotide polymorphisms in the coding region of human CXC-chemokine receptors CXCR1, CXCR2 and CXCR3"Genes immun.. 1. 330-337 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawasaki A, et al.: "Independent contribution of HLA-DRB1 and TNF? Promoter polymorphisms to the susceptibility to Crohn's disease"Genes Immun.. 1. 351-357 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchiya N, et al.: "Analysis of the association of HLA-DRB1, TNF? Promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test"Genes Immun.. 2(6). 317-322 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato M, et al.: "Identification of novel single nucleotide substitutions in the NKp30gene expressed in human natural killer cells"Tissue Antigens. 58(4). 255-258 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyogoku C, et al.: "Fc? Receptor gene polymorphisms in Japanese patients with systemic lupus erythematosus : Contribution of FEGR2B to genetic susceptibility"Arthritis Rheum.. 46(5). S21-S30 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawasaki A, et al.: "Analysis on the association of human BLYS (BAFF, TNFSF13B) polymorphisms with systemic lupus erythematosus and rheumatoid arthritis"Genes Immun.. 3(7). 424-429 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchiya N, et al.: "Variations in immune response genes and their associations with multifactorial immune disorders"Immunological Reviews. 190. 169-181 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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