• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

Establishment of a solar UV dosimeter based on the photoisomerization of (6-4)photoproducts to the Dewar isomers.

Research Project

Project/Area Number 12558059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 環境影響評価(含放射線生物学)
Research InstitutionKanazawa University

Principal Investigator

NIKAIDO Osamu  Kanazawa University, Faculty of Pharmaceutical Sciences, Professor., 薬学部, 教授 (60019669)

Co-Investigator(Kenkyū-buntansha) ISHIGAKI Yasuhito  Kanazawa University, Faculty of Pharmaceutical Sciences, Research Associate., 薬学部, 助手 (20232275)
MATSUNAGA Tsukasa  Kanazawa University, Faculty of Pharmaceutical Sciences, Associate Professor., 薬学部, 助教授 (60192340)
Project Period (FY) 2000 – 2001
KeywordsDNA damage / cyclobutane pyrimidine dimer (CPD) / (6-4)photoproduct / immuno-dot-blot (IDB) / risk assessment / Dewar photoproduct / monoclonal antibody / photoisomerization
Research Abstract

We established a solar UV dosimeter measuring the UV inteiasiiy of wavelengths ranging from 300 to 340 nm.
The solar UV, especially UVB(290-320nm)induces cyclobutane pyrimidine dimers(CPD)and(6-4)photoproduct(64P) in cellular DNA. Furthemore, the UV wavelengths ranging from 300 to 340 nm in solar light are known to efficiently photoisomerize 64P to its Dewar isomer(DwP). The latter is said to be highly mutagenic. Thus, we need to establish a dosimeter measuring the accumulation of the DwP in DNA for the risk assessment of solar UV. However, none of dosimeters measuring the wavelengths from 300 to 340 nm has been established so far. To establish a new UV dosimeter, we carried out the experiments nmentioned below,
1) We blotted the DNA irradiated with 100 J/m^2 of UVC on a nylon membrane. The membrane was then exposed to various doses of monochromatic UV light from the Okazaki Large Spectrograph. After exposure, the membrane was treated with DEM-1 antibody immnohisitochemically and the color intensity was assayed (immuno-dot-blot : IDB). The results obtained so far revealed the photoisomerization of DwP from 64P efficiently occurs at 320 nm.
2) By using the same method (IDB), we measured the UV doses from 300 to 340 nm emitted from the Mylar-filtered UVB lamp (Toshiba FL-20SE). The color intensity of the blotted DNA on the nylon membrane increased lmearly with increasing dosed of UVB. Thus, we succeeded to produce a new solar UV dosimeter measuring the wavelengths from 300 to 340 nm.

  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Otoshi, E.: "Respective role of cyclobutane pyrimidine dimers, (64)photoproducts, and minor photoproducts in ultraviolet mutagenesis of repair deficient xeroderma pigmentosum A cells"Cancer Res.. 60(6). 1729-1735 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ide, F.: "In vivo detection of ultraviolet photoproducts and their repair in Purkinje cells"Lab. Invest.. 80(4). 465-470 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horiki, S.: "Protective effects of sunscreening agents on photocarcinogenesis, photoageing, and DNA damage in XPA gene knockout mice"Arch. Delmatol. Res.. 292(10). 511-518 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Torizawa, T.: "DNA binding mode of the Fab fragment of a monoclonal antibody specific for cyclobutane pyrimidine dimer"Nucleic Acids Res.,. 28(4). 944-951 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Perdiz, D.: "Distribution and repair of bipyrimidine photoproducts in solar UV-irradiated mammmalion cells. Possible role of Dewar photoproduct in solar mutagenesis"J. Biol. Chem.,. 275(35). 26732-26742 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoyama, H.: "Crystal structure of the 64M-2 antibody Fab fragment in complex with a DNA dT(6-4) T photoproduct formed by ultraviolet radiation"J. Mol. Biol.,. 299(3). 711-723 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Cario-Andre, M.: "Studies on epidermis reconstructed with and without melanocytes : Melanocytes prevent sunburn cell formation but not appearance of DNA damaged cells in fair-skinne caucasians"J. Invest. Dermatol.,. 155(2). 193-199 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ray, AJ.: "The spectrum of mitochondrial DNA deletions in a ubiquitous marker of ultraviolet radiation exposure in human skin"J. Invest. Dermatol.,. 155(4). 674-679 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi, S.: "Formation of DNA lesions in cucumber cotyledons exposedto UV radiation"Environ. Sci.. 13. 351-355 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otochi, E.: "Respective role of cyclobutane pyrimidine dimers, (6-4)photopfoducts, and minor photoproducts in ultraviolet mutagenesis of repair deficient xeroderma pigmentosum A cells"Cancer Res.,. 60(6). 1729-1735 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ide, F.: "In vivo detection of ultraviolet photoproducts and their repair in Purkinje cells"Lab. Invest.,. 80(4). 465-470 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horiki, S.: "Protective effects of sunscreening agents on photocarcinogenesis, photoageing, and DNA damage in XPA gene knockout mice"Arch. Delmatol. Res.,. 292(10). 511-518 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Torizawa, T.: "DNA binding mode of the Fab fragment of a monoclonal antibody specific for cyclobutanee phrimidine dimer"Nucleic Acids Res.,. 28(4). 944-951 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Perdiz, D.,: "Distribution and repair of bipyrimidine photoproducts in solar UV-irradiated mammalian cells. Possible role of Dewar photoproducts in solar mutagenesis"J. Biol. Chem.,. 275(35). 26732-26742 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoyama, H.: "Crystal strucfure of the 64M-2 antibody Fab fragment in complex with a DNA dI(6-4) T photoproduct formed by ultravioletradiation"J. Mol. Biol.,. 299(3). 711-723 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cario-Andre, M.: "Studies on epideraiis reconstructed with and without melanocytes : Melanocytes prevent sunburn cell formation but not appearance of DNA damaged cells in fair-skinned caucasians"J. Invest. Dermatol.,. 115(2). 193-199 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ray, AJ.: "The spectrum of mitocheondrial DNA deletions in a ubiquitous marker of ultraviolet radiation exposure in human skin"J. Invest. Dermatol.,. 115(4). 674-679 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi, S.: "Formation of DNA lesions in cucumber cotyledons exposed to UV radiation"Environ. Sci.,. 13. 351-355 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Budiyanto, A.: "Protective effect a topically applied olive oil against photocarcinogenesis following UVB exposure of mice"Carcino-genesis. 21(11). 2085-2090 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satou, K.: "Efficient chemical synthesis of a pynmidine (6-4)pyrimidone photo-product analog and its properties"Tetrahedorn Letters,. 41. 2175-2179 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakamoto, A.: "Immunoxpression of ultraviolet photoproducts and p53 mutation analysis in atypical fibroxanthoma and supferficial malignant fibrous histiocytoma"Modern Patol.,. 14. 581-588 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyosawa, K.: "Amplified UvrA protein can ameliorate the ultraviolet sensitivity of an Eschrichia coli recA mutant"Mutation Res.,. 487. 149-156 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mone, M. J.: "Local UV-induced DNA damage in cell nuclei results in local transcription inhibition"EMBO Rep.,. 2. 1013-1017 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumi, S.: "In situ visualization of ultraviolet-light-induced DNA damage repair in locally irradiated human fibroblasts"J. Invest. Dermatol.,. 117. 1156-1161 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, S.: "The relationship between UVB screening and cytoprotection by microcorpuscular ZnO or ascorbate against DNA photodamage and membrane injuries in keratinocyte by oxidative stress"J. Photochem. Photobiol., B.,. 64. 27-35 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wakasugi, M.: "DDE stimulates the excision of cyclobutane pyrimidine aimers in vitro in concert with XPA and RPA"J. Biol. Chem.,. 276. 15434-15440 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, M.: "Effects of photoreactivation of cyclobutane pyrimidine aimers and pyrimidine (6-4) pyrimidone photopioducts on ultraviolet rhutagenesis in SOS-induced repair-deficient Escherichia coli"Mutagenesis,. 16(1). 1-6 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wakasugi, M.: "DDB accumulates at DNA damage sites immediately after UV-irradiation and directly stimulates nucleotide excision repair"J. Biol. Chem.,. 16(1). 1637-1640 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishigaki, Y.: "Evidence for a pioneer round of mRNA translation : mRNAs subject to nonsense-mediated decay in mammalian Cells are bound by CBP80 and CBP20"Cell,. 106. 607-617 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katayama, S.: "Fission yeast F-box protein Pof3 is required for genomic instability and telomere function"Mol. Biol. Cell,. 13. 211-224 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi