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2002 Fiscal Year Final Research Report Summary

Establishment of Limb Regeneration System in Mouse

Research Project

Project/Area Number 12558086
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

IDE Hiroyuki  Tohoku University, Graduate School of Life Sciences, Prof., 大学院・生命科学研究科, 教授 (70022704)

Co-Investigator(Kenkyū-buntansha) KOYAMA Yoichi  Nippi Co. Institute for Biomatrix Res., Head Researcher, バイオマトリックス研究所, 主任研究員
TAMURA Koji  Tohoku University, Graduate School of Life Sciences, Assoc. Prof., 大学院・生命科学研究科, 助教授 (70261550)
YAMAMOTO Hiroaki  Tohoku University, Graduate School of Life Sciences, Assoc. Prof., 大学院・生命科学研究科, 助教授 (40174809)
Project Period (FY) 2000 – 2002
Keywordslimb / FGF / BMP / Mouse / regeneration
Research Abstract

Induction of limb regeneration in mouse was studied to establish limb regeneration system in higher vertebrates.
Forelimbs of new-born mouse were amputated at various level, and growth factor proteins were locally applied to the amputation sites after two days.
The regeneration potency changed depending on amputation level. The limbs amputated at forearm and hand level induced the hypertrophy of stump bone tissues. The limbs amputated at wrist level induced the formation of cartilage tissues at the distal margin of stump tissues.
FGF-2 and FGF-4 protein applied to the amputation sites promoted the hypertrophy of bone tissues. No promotion was observed by the local application of FGF-8 and FGF-10. BMP-2 and BMP-4 induced the formation of distal structures at the amputation site.
Genes of FGF receptors were applied to the amputation sites by a gene-gun. Expression of GFP injected simultaneously was observed, but no changes in cartilage pattern occurred.
These results suggest the local proliferation and differentiation was induced in some FGFs and BMPs, although no stimulation was observed by FGF-10, which is known to induce limb regeneration in Xenopus laevis.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Yokoyama, H.: "Anteroposterior axis formation in Xenopus limb bud recombinants : A model of pattern formation during limb regeneration"Developmental Dynamcs. 225. 277-288 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito, D.: "Specification and detemination of limb identity : Evidence for inhibitory regulation of Tbx gene expression"Development. 129. 211-220 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoyama, H.: "FGF-10 stimulates limb regeneration ability in Xenopus laevis"Developmental Biology. 233. 72-79 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuda, H.: "An epidermal signal regulates Lmx-1 expression and dorso-ventral pattern during Xenopus limb regeneration"Developmental Biology. 229. 351-362 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoyama, H.: "Anteroposterior axis formation in Xenopus limb bud recombinants: A model of pattern formation during limb regeneration"Developmental Dynamics. 225. 277-288 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, D.: "Specification and determination of limb identity: Evidence for inhibitory regulation of Tbx gene expression"Development. 129. 211-220 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoyama, H.: "FGF-10 stimulates limb regeneration ability in Xenopus laevis"Developmental Biology. 233. 72-79 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda, H.: "An epidermal signal regulates Lmx-1 expression and dorso-ventral pattern during Xenopus limb regeneration"Developmental Biology. 229. 351-362 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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