2003 Fiscal Year Final Research Report Summary
Development of artificial periodontal membranes for periodontal disease using polyphosphate
| Project/Area Number |
12558099
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Matsumoto Dental University (2002-2003)
Hokkaido University (2000-2001) |
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Principal Investigator |
YAMAOKA Minoru Matsumoto Dental University, School of Dentistry, Professor, 歯学部, 教授 (50064671)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBA Toshikazu Fujirebio Inc., Research and Development, Research Director, 先端研究部, リサーチディレクター (60241303)
TANAKA Hitoshi Matsumoto Dental University, School of Dentistry, Assistant Professor, 歯学部, 講師 (50267788)
UEMATSU Takashi Matsumoto Dental University, Graduate School of Oral Medicine, Associate Professor, 大学院・歯学独立研究科, 助教授 (40203476)
ERATA Tomoki Hokkaido University, Faculty and Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (30213581)
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| Project Period (FY) |
2000 – 2003
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| Keywords | polyphosphate / artificial periodontal membrane / tissue regeneration / periodontal tissue / osteoblast |
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| Research Abstract |
Inorganic polyphosphates [Poly(P)] are chains of phosphate molecules and are often distributed in osteoblasts. The purpose of this study was to clarify the effects of Poly(P) on periodontal regeneration. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly(P), and its gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain reaction and von Kossa staining. Alkaline phosphatase activity was also determined. The potential role of Poly(P) in bone formation was assessed using a rat alveolar bone regeneration model. Poly(P) exhibited antibacterial activity against Prophyromonas gingivalis and Streptococcus mutans, induced expression of osteopontin and osteocalcine and increased alkaline phosphatase activity in MC 3T3-E1 cells. Promotion of alveolar bone regeneration was observed locally in Poly(P)-treated rats. These findings suggest that Poly(P) plays roles of osteoblastic differentiation, activation and bone mineralization and possesses antibacterial activity. Thus, local delivery of poly(P) may have adjunctive therapeutic benefits in periodontal disease.
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