2002 Fiscal Year Final Research Report Summary
Development of synchrotron-radiation vacuum-ultraviolet circular dichroism spectrophotometer and its application for structural analysis of biomaterials
| Project/Area Number |
12559007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
GEKKO Kunihiko Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10023467)
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| Co-Investigator(Kenkyū-buntansha) |
OHMAE Eiji Hiroshima Univ., Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (30284152)
KATAYANAGI Katsuo Hiroshima Univ., Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (20291479)
TANIGUCHI Masaki Hiroshima Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10126120)
FUKAZAWA Tomoyuki JASCO Corp. Spectroscopic Products Division, Manager, 第一技術部, 技術担当マネージャー
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| Project Period (FY) |
2000 – 2002
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| Keywords | synchrotron radiation / vacuum-ultraviolet / circular dichroism / spectrophotometer / structural analysis / biomaterials |
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| Research Abstract |
In order to analyze the structure of biomaterials based on the higher energy transition of chromophores, we constructed the vacuum-ultraviolet circular dichroism (VUVCD) spectrophotometer which is capable of measuring the CD spectra in the wavelength region of 310-140 nm under high vacuum in the solution, using the synchrotron radiation source (HiSOR) at Hiroshima University Synchrotron Radiation Center (HSRC). The signal-to-noise ratio of the spectra was improved by exchanging the grating mirror in the monochromator. The performance of the constructed system was confirmed to be satisfactory by monitoring the CD spectra of ammonium d-camphor-10-sulfonate. The VUVCD spectra of eight amino acids were published in Chemistry Letters, 2002, 826. Further we constructed the temperature control unit of the sample cell with a Peltier thermoelectric element to keep the sample in the temperature range from -30 to 70℃. This system was applied for studying the cold-denaturation of myoglobin and the performance of the system were published in Analytical Sciences 19, 129 (2003). Usefulness of the constructed spectrophotometer was further confirmed from the measurements and theoretical assignment of VUVCD spectra of various sugars and proteins. This VUVCD spectrophotometer will be opened for common utilization from April of 2003.
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