• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

Development of automated high-resolutoin HLA-DNA typing system using DNA tips

Research Project

Project/Area Number 12559008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 広領域
Research InstitutionTokai University

Principal Investigator

INOKO Hidetoshi  Tokai University School of medicine, Professor, 医学部, 教授 (10101932)

Co-Investigator(Kenkyū-buntansha) SUZUKI Osamu  Nisshinbo Industries, INC. Tokyo Research Center, Senior Researcher, 東京研究センター, 主任研究員
ANDO Asako  Tokai University School of medicine, Assistant Professor, 医学部, 講師 (40101935)
Project Period (FY) 2000 – 2001
KeywordsHLA / DNA typing / DNA tip / genotype automated decision / 自動判定
Research Abstract

In order to establish the automated high-resolution HLA typing system, we have developed the PCR-SSOP (polymerase chain reaction - sequence specific oligonucleotide probe) method using microarray technique.
In this study, a total of 365 allelic polymorphisms of HLA-DRB genes were searched. A total of 20 sets of primer pairs were used for specific PCR amplification specific for HLA-DRB1, -DRB3, -DRB4 and -DRB5 genes, then hybridized with 124 oligonucleotide probes which were designed to be complimentary to the target olymorphic region of each primer pair. Each 5' primer employed in this study were labeled with fluorescence dye, which can be detected by the scanner of microarray system in the case of successful hybridization with the probe spotted on a glass tip.
As a result, 14 sets of primer pairs and 103 of probes adopted to these focus on a Japanese population, were successfully applied to genotyping of 70 genomic DNAs extracted from 20 Japanese homozygous cells and 50 individual. Identity between genotypes of 20 homozygous DNAs typed by this method and those obtained by PCR-RFLP (restriction fragment length polymorphism) were 80%, likewise, 69% for 50 individuals. It will be necessary to modify probe design to increase a accuracy, however, it will become suitable for HLA allele typing if low-background can be attained to prevent false signals of heterozygous.

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Shiina T., et al.: "The beta 1, 3-galactosyltransferase-4 (B3GALT4) gene is located in the centromeric segment of the human MHC class II region"Immunogenetics. 51. 75-78 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Teraoka Y., et al.: "Genetic polymorphisms in the cell growth regulated gene, SC1 telomeric of the HLA-C gene and lack of association with psoriasis vulgaris"Tissue Antigens. 55. 206-211 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamura K., et al.: "Induction of autoimmune encephailtis by proteolipd protein 95-116-specilic T cells from HLA-DR2 (DRB1^*1502) transgenic mice"J Clinical Investigation. 105. 977-984 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi T., et al.: "Significance of transporter associated with antigen processing 2 (TPP2) gene polymorphism in living-related renal transplantation"Hum Immunol. 61. 670-674 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naruse TK., et al.: "HLA-DQ1^*0601 is primarily associated with the susceptibility to cardiac sarcoidosis"Tissue Antigens. 56. 52-57 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ota M., et al.: "On the MICA deleted-MICB null, HLA-B4801 haplotype"Tissue Antigens. 56. 268-273 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuwana M., et al.: "HLA class II alleles in Japanese patients with immune thrombocytopenic purpura Associations with anti-platelet glycoprotein autoantibodies and responses to splenectomy"Tissue Antigens. 56. 337-342 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuzaka Y., et al.: "New polymorphic microsatellite markers in the human MHC class II region"Tissue Antigens. 56. 492-500 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito S., et al.: "Allele frequencies and haplotypic associations defined by allefic DNA typing at HLA class I and class II loci in the Japanese population"Tissue Antigens. 56. 522-529 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 成瀬妙子, 猪子英俊: "質量分析法(mass spectrometry)による遺伝子多型タイピング"実験医学. No12増刊号. 1602-1607 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安西達也, 猪子英俊: "MHC class II"臨床免疫. 34. 176-183 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 椎名隆, 猪子英俊: "HLA領域のゲノムシークエンス"今日の移植. 13. 311-317 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 成瀬妙子, 猪子英俊: "やさしい遺伝子情報解説-ヒトゲノムから遺伝子治療まで"青鴎. 365. 66-71 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikewaki I., et al.: "A unique monoclonal antibody mNI-11 rapidly enhances spread formation in human umbilical vein endothelial cells"J Clinicai Immunology. 20. 317-324 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ota M., et al.: "A second susceptibility gene for developing rheumatoid arthritis in the human MHC is localized within a 70-kb interval telometric of the TNF genes in the HLA class III region"Genomics. 71. 263-270 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kulski JK., et al.: "The association between HLA-A alleles and an Alu dimorphism near HLA-G"J Mol Evol. 53. 114-123 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Romphruk AV., et al.: "Diversity of MICA (PERB11.1) and HLA hapltotypes in Northeastern Thais"Tissue Antigens. 58. 83-89 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiina T., et al.: "Genomic anatomy of a premiar Major Histocompatibility Complex paralogous region onchrormosome 1q21-22"Genome Research. 11. 789-802 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中島舞子, 成瀬妙子, 猪子英俊: "HLAクラスII抗原提示におけるHLA-DOの役割"臨床免疫. 35. 661-666 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sano K., et al.: "The absebce of disease-specific polymorphisms within the HLA-B51 gene that is the susceptible locus for Behcet's disease"Tissue Antigens. 58. 77-82 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanaya K et al.: "Major Histocompatibility Complex 2000"Springer. 561 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yabuki K et al.: "HLA testing in patients with uveitis"Springer. 367 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 成瀬妙子, 猪子英俊: "新移植免疫学"中外医学社. 20 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松坂恭成, 猪子英俊: "医療機器センター15周年記念誌"医療機器センター. 4 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiina T et al: "The beta 1, 3-galactosyltransferase-4 (B3GALT4) gene is located in the centromeric segment of the human MHC class 2 region"Immunogenetics. 51. 75-78 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Teralka Y et al: "Genetic polymorphisms in the cell growth regulated gene, SCI telomeric of the HLA-C gene and lack of association with psoriasis vulgaris"Tissue Antigens. 55. 206-211 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamura K et al: "Induction of autoiimmune encephalitis by proteolipid protein 95-116-specifie T cells from HLA-DR2 (DRB1*1502) transgenic mice"J Clinical Investigation. 105. 977-984 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi T et al.: "Singnificance of transporter associated with antigen processing 2(TAP2) gene polymorphism in living-related renal transplantation"Hum Immunol. 61. 670-674 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naruse TK et al.: "HLA-DQ1*0601 is primarily associated with the susceptibility to cardiac sarcoidosis"Tissue Antigens. 56. 52-57 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ota M et al.: "On the MICA deleted-MICB null, HLA-B4801 haplotype"Tissue Antigens. 56. 268-273 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuwana M et al.: "HLA class 2 alleles in Japanese patients with immune thrombocytopenic purpura. Associations with anti-platelet glycoprotein autoantibodies and responses to splenectomy."Tissue Antigens. 56. 337-343 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuzaka Y, et al: "New polymorphic microsatellite markeres in the human MHC"Tissue Antigens. 56. 492-500 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito S et al.: "Allele frequencies and haplotypic associations defiend by allelic DNA typing at HLA class 1 and 2 loci in the Japanese population."Tissue Antigens. 56. 522-529 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naruse TK et al.: "A novel method of detecting genetic polymorphisms using matrix-assisted laser desorption ionization time of flight mass spectromety."Experimental Medicine. 18. 1602-1607 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anzai T et al.: "MHC class 2"Clinical Immunology. 34. 176-183 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiina T et al: "Genome Soquence of HLA region."Transplantation Now. 13. 311-317 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naruse TK et al.: "How to analyze genetic informations from human genome to gene therapy"Seioh. 365. 61-71 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikewaki et al.: "A unique monoclonal antibody mNI-11 rapidly enhances spread formation in human umbilical vein endothlial cells."J Clinical Immunology. 20. 314-324 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ota M et al.: "A second susceptibility gene for developing rheumatoid arthritis in the human MHC is localized within a 70-kb interval telomeric of the TNF genes in the HLA class 3 region."Genomics. 71. 263-270 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kulski J K et al.: "The association between HLA-A alleles and an Alu dimorphism near HLA-G."J Mol Evol. 53. 114-123 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Romphruk AV et al.: "Diversity of MICA(PERB11.1) and HLA hapltotypes in Northeastern Thais."Tissue Antigens. 58. 83-89 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiina T et al: "Genomic anatomy of a premiar Mafor Histocompatibility Complex paralogous region on chrormosome 1021-22."Genome Research. 11. 789-802 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sano K et al.: "The absebce of disease-specific polymorphisms within the HLA-B51 gene that is the susceptible locus for Behcet's disease"Tissue Antigens. 58. 77-82 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakashima M et al.: "HLA-DO: a specialized role as a co-chaperone with HLA-DM in the 2LA Class 2 antigen processing pathway"Clinical Immunology. 35. 661-666 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yabuki K et al.: "HLA testing in patients with uveitis"International Ophthalmology Clinics. 367 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanaya K et al.: "Disribution of polypurine/polypyrimidine tract sequences in the human MHC region and their possible functions"Major Histcompatibility Complex. 561 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naruse TK et al.: "HLA DNA typing : it's clinical role on Transplantation"New Transplantation Immunology. 140-159 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuzaka Y, et al: "New technologies for DNA analysis and research of functional genome"Japan Association for the Advancement of Medical Equipment. 82-85 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi