Conservation and Analyzes of Genetic Information of Asian Ethnic Groups

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12575015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: 人類学(含生理人類学)
• Research Institution: The University of Tokyo
• Principal Investigator: ISHIDA Takafumi The University of Tokyo, Graduate School of Science, Associate Professor, 大学院・理学系研究科, 助教授 (20184533)
• Project Period (FY): 2000 – 2002
• Keywords: Asia / Ethnic Group / Genetic Information / Cell bank / DNA bank

Research Abstract

The aim of this study is to conserve genetic information of Asian ethnic groups especially small sized populations in the form of a cell bank and DNA bank. For this, we have conducted field studies in Indonesia, Malaysia and Thailand.
1. Field study: for the population genetic study, specimens were collected from Sumbanese, Timorese, Moken and Mani. A total of 400 specimens were collected and 250 cell lines were established.
2. Study on Genome diversity: Polymorphisms of the following genes, SDF1, MC1R, AIMland p53, were studied among these populations with other Asian and Oceanian populations.
3. Malaria related genes: Prevalence of 27-bp deletion of the 3 protein gene arid G6PD deficiency was studied in Sumbanese in the presence of the parasitemia data.
4. Epstein-Barr virus associated peripheral T-cell proliferative diseaseis: A rapid and sensitive plasma DNA test for this disease entity was established.

Research Products

• [Publications] Kimura, R. et al.: "The SDF1-G801A polymorphism is not associated with SDF1 gene expression in Epstein-Barr virus-transformed lymphoblastoid cells"Genes and Immunity. (in press). (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kimura, M. et al.: "Malaria species and Southeast Asian ovalocytosis defined by a 27-bp deletion in the erythrocyte band 3 gene"Southeast Asian J. Trop Med Public Health. 33. 4-6 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kimura, R. et al.: "Anthropological implication of the SDF1-3'A allele distribution in Southeast Asia and Melanesia"Journal of Human Genetics. 47. 117-121 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakayama, K. et al.: "Distinctive distribution of AIM1 polymorphism among major human populations with different skin color"Journal of Human Genetics. 47. 92-94 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oota, H. et al.: "Residence patterns influence human Mt DNA and Y-chromosome variation"Nature Genetics. 29. 20-21 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shimizu, Y. et al.: "Sero-and molecular-typing of Duffy blood group in Southeast Asians and Oceaniams"Human Biology. 72. 511-518 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kimura, R., Nishioka, T., and Ishida, T.: "The SDF1-G801A polymorphism is not associated with SDF1 gene expression in Epstein-Barrvirus-transformed lymphoblastoid cells"Genes and Immunity. in press.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mori, A., Takao, S., Pradutkanchana, J., Kietthubthew, S., Mitarnun, W. and Ishida, T.: "High tumor necrosis factor-α leves in the patients. With Epstein-Barr virus-associated peripheral T-cell proliferative disease/lymphoma"Leukemia Res.. 27. 493-498 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kantaputra, P.N., Yamasaki, K., Ishida, T., Kishino, T., and Niikawa, N.: "A dominantly inherited malformation syndrome with short stature, upper limb anomaly, minor craniofacial anomalies, and absence of TBX5 mutations : report of a Thai family"American Journal of Medical Genetics. 111. 301-306 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kimura, M., Soemantri, Ag., And Ishida, T.: "Malaria species and Southeast Asian ovalocytosis defined by a 27-bp deletion in the erythrocyte band 3 gene"Southeast Asian Journal of Tropical Medicine and Public Health. 33. 4-6 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kimura, R., Soemantri, Ag., Settheetham-I. W., Ohtsuka, R., Inaoka, T., Pookajorn, S., Tiwawech, D., Duangchan, P., and Ishida, T.: "Anthropological implication of the SDF1-3'A allele distribution in Southeast Asia and Melanesia"Journal of Human Genetics. 47. 117-121 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakayama, K., Fukamachi, S., Kimura, H., Koda, Y., Soemantri, Ag., And Ishida, T.: "Distinctive distribution of AIM1 polymophism among major human populations with different skin color"Journal of Human Genetics. 47. 92-94 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishida, T., Takao, S., Settheetham-I., W., and Tiwawech, D.: "Prevalence of hepatitis B and C virus infection in rural ethnic populations of northern Thailand"Journal of Clinical Virology. 24. 31-35 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oota, H., Settheetham-I., W., Tiwawech, D., Ishida, T., and Stoneking, M.: "Residence patterns influence-human MtDNA and Y-chromosome variation"Nature Genetics. 29. 20-21 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shimizu, Y., Ao, H., Soemantri, Ag., Tiwawech, D., Settheetham-Ishida, W., Kayame, O.W., Nishioka T. and Ishida, T.: "Sero- and molecular-typing of Duffy blood group in Southeast Asians and Oceanians"Human Biology. 72. 511-518 (2000)
  • Description: 「研究成果報告書概要(欧文)」より