2002 Fiscal Year Final Research Report Summary
Pathological and epidemiologic study on nasal NK/T-cell lymphoma
| Project/Area Number |
12576004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Human pathology
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| Research Institution | Osaka University |
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Principal Investigator |
AOZASA Katsuyuki Osaka University Graduate School of Medicine Professor, 医学系研究科, 教授 (30115985)
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| Co-Investigator(Kenkyū-buntansha) |
HONGYO Tadashi Osaka University Graduate School of Medicine Assistant Professor, 医学系研究科, 助教授 (90271569)
TAKAKUWA Tetsuya Osaka University Graduate School of Medicine Assistant Professor, 医学系研究科, 助教授 (40244933)
TOMITA Yasuhiko Osaka University Graduate School of Medicine Assistant Professor, 医学系研究科, 助教授 (60263266)
KOIYA Shizuo Urasce General Hospital Medical Staff, 医員(臨床研究)
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| Project Period (FY) |
2000 – 2002
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| Keywords | east Asia / nasal lymphoma / NK / T-cell / Ebvirus / prestidde / genemutaticrs / p53 / epldemiclcgy |
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| Research Abstract |
Gene mutation analysis
(1) Mutations of p53 gene(exon5-8) were analyzed in cases with nasal Nk/T-cell l lymphoma(NKTCL)(19from China(Bejin)and 23 from Japan) with use of polymerase chaln reaction(PCR)-slngle strand conformation polyrnorphism followed by direct sequencing. P53 gene mutations were found in 20 cases(47.6%) with missense mutations in 60% of cases. Mutation frequency in Bejiing(63%) washigher than that in Japan(39%)
(2) c-kit gene mutations were examined in 23 cases with nasal NKTCL(14 from Bejiing and a from Japan) by PCR-SSCP analysis. Mutation frequency in China(71.4%) much higher than than that in Japan(22%). Mutated c-kit genes transfected into 293T cell did not generate constitutive activation of c-kit, thus nota gain-of-function mutation
(3) p53(exon4-8), c-kit(exon 11, 17), k-ras(exon1, 2), and β-catenin gene(exon3), mutations were examined in 20 cases with nasal NKTCL from northeast dlstrict of China, P53 mutations were found in 40% of cases with significantly lower involvement of exon4 than Japan Mutations of c-kit gene were found in only 5% of cases. Mutations of k-ras and β-catenin were found 5% and 30% of cases, respectively. These findings showed that mutation frequency and pattern of suppressor gene and oncogenes were various by direct, suggestion that presence of different environment factors for developrnent of nasal NK/T cell lymphoma.
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[Publications] Hoshida Y, Hongyo T, Jia X, He Y, Hasui K, Dong Z, Luo W-J, Ham MF, Nomura T, Aozasa K.: "Analysis of p53, K-ras, c-kit, and β-catenin gene mutations in sincnasal NK/T cell lyrnphonma in ncrtheast district of China"Cancer Dci. in press.
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