2001 Fiscal Year Final Research Report Summary
Mechanism of zinc-insertion into porphyrin ring in acidophilic photosynthetic bacteria
| Project/Area Number |
12640629
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TAKAMIYA Ken-ichiro Tokyo Institute of Technology, Grad. School Biosci. Biotech., Professor, 大学院・生命理工学研究科, 教授 (80037259)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Hiroshi Tokyo Institute of Technology, Grad. School Biosci. Biotech., Assistant Professor, 大学院・生命理工学研究科, 助手 (80301175)
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| Project Period (FY) |
2000 – 2001
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| Keywords | zinc-bacteriochlorophyll / bacteriochlorophyll / acidophilic photosynthetic bacteria / magnesium-chelatase / farrochelatase / chlorophyll biosynthesis / Acidiphilium rubrum |
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| Research Abstract |
The aim :
Final aim of this research is to elucidate the mechanism of biosynthesis of zinc-bacteriochlorophyll in acidophilic photosynthetic bacteria.
Results and scope :
1. In Acidiphilium rubrum, there was a single copy of magnesium chelatase subunit genes and these were functional in this bacterium.
2. Only magnesium-protoporphyrin IX and its monomethyl ester accumulated but not zinc-protoporphyrin IX or its monomethylester in the bacterium. Protoporphyrin IX monomethyl ester was also detected as an intermediate. These suggested magnesium was exchanged by zinc after magnesium insertion to form zinc-bacteriochlorophyll.
3. Whether the exchange takes place in enzymatically or non-enzymatically could not be determined, but it was possible that in the low pH environment, where zinc-bacteriochlorophyll might be formed, the exchange take places very easily and non-enzymatically.
4. We tried to detect intermediates containing zinc-tetrapyrroles in this bacterium to identify the exchange reaction but so far we could not.
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[Publications] Suzuki, T., Masuda, T., Singh, D.P., Tan, F-C., Tsuchiya, T., Shimada, H., Ohta, H., Smith, A.G., Takamiya, K.: "Two types of ferrochelatase in photosynthetic and non-photosynthetic tissues of cucumber ; their difference in phylogeny, gene expression and localization"J. Biol. Chem.. 277(7) (in press). (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takamiya, K., Masuda, T., Inoue, K., Masuda, M., Nagura, A., Nagayama, M., Tamaki, A., Ohta, H., Shimada, H. and Takamiya, K.: "2000 Biosynthesis of zinc-containing bacteriochlorophyll a"Porphyrins. 9(3-4). 227-233 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, T., Masuda, T., Masuda, T., Inokuchi, H., Shimada, H., Ohta, H. & Takamiya, K.: "Overexpression, enzymatic properties and tissue localization of a ferrochelatase of cucumber"Plant Cell Physiol.. 41. 192-199 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Masuda, T., Inoue, K., Masuda, M., Nagayama, M., Tamaki, A., Ohta, H., Shimada, H., & Takamiya, K.: "Magnesium insertion by magnesium chelatase in the biosynthsis of zinc-bacteriochlorophyll a in an aerobic acidophilic bacterium Acidiphilium rubrum"J. Biol. Chem.. 274. 33594-33600 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nishimura, K., Shimada, H., Shinmen, T., Obayashi, T., Masuda, T., Ohta, H. and Takayama, K.: "Photosynthetic regulatory gene clyuster in an aerobic photosynthetic bacterium, roseobacter denitrificans"J. Gen. Appl. Microbiol.. 45. 129-134 (1999)
Description
「研究成果報告書概要(欧文)」より
